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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2007, Vol. 1 Issue (1) : 62-66     DOI: 10.1007/s11703-007-0011-1
Research article |
Fertilization in Brassica campestris ssp. pekinensis and its duration of each stage
Jie PENG,Jiaheng SHEN()
Department of Biology, College of Life and Environment Science, Harbin Normal University, Harbin 150080, China
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Abstract

This paper reports the process of fertilization in Brassica campestris ssp. pekinensis and the duration of each stage. The results are as follows: (1) Pollen germinates on stigma 2-3 h after pollination. (2) 4-8 h after pollination, pollen tube grows in the style. (3) 8-14 h after pollination, pollen tube grows in the ovary and gets into the ovule via the micropyle. (4) 16 h after pollination, one sperm nucleus moves to the egg and enters it. (5) The sperm nucleus adheres to the nuclear membrane of the egg 18 h after pollination. (6) 20 h after pollination, it enters the egg nucleus and male chromatin gradually disperses and 24 h after pollination, a male nucleolus appears. A large female nucleolus and a small male nucleolus occur in the nucleus of the fertilized egg, and zygote formed. The dispersing of sperm chromatin in the egg nucleus takes about 4 h. (7) 32-34 h after pollination, the division of zygote begins. The dormancy stage of the zygote lasts for about 8-10 h. (8) The pair polar nuclei lie in the chalazal end of the egg before fertilization, which may fuse into a secondary nucleus or not. (9) 16-18 h after pollination, the sperm nucleus moves to the polar nuclei or the secondary nucleus. 18 h after pollination, the sperm nucleus adheres to the nuclear membrane of the polar nuclei or that of the secondary nucleus. (10) 20 h after pollination, the sperm nucleus enters one of the polar nuclei or the secondary nucleus and a triple fusion takes place. The process of fusion is similar to the karyogamy but faster. The dispersing of the sperm chromatin in the polar nucleus or secondary nucleus takes about 2 h. (11) 22 h after pollination, the primary endosperm nucleus formed. The female and male nucleoli cannot fuse with each other before mitotic division of the primary endosperm nucleus. (12) 24 h after pollination, the division of the primary endosperm nucleus actually takes place.

Keywords Brassica campestris ssp. pekinensis      fertilization     
Issue Date: 22 February 2016
 Cite this article:   
Jie PENG,Jiaheng SHEN. Fertilization in Brassica campestris ssp. pekinensis and its duration of each stage[J]. Front. Agric. China, 2007, 1(1): 62-66.
 URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-007-0011-1
http://academic.hep.com.cn/fag/EN/Y2007/V1/I1/62
Plate I  Fertilization process of Brassica campestris ssp. pekinensis

1: Three-celled mature pollen; 2: Papillose cells; 3: Transmitting tissue; 4: The pollen tube grows in the intercellular space of papillosem cells; 5: Pollen tube grows on the surface of pseudoseptum; 6: Pollen tube protrudes from the pseudoseptum and grows along the surface of pseudoseptum towards ovule; 7: A pair of ellipsoid sperms in pollen tube; 8: Two synergids; 9: Egg; 10: A pair of polar nuclei underneath an egg; 11: A pair of sperms released into the space between the egg and polar nuclei; 12: Lens-shaped sperm nucleus on the surface of the egg nucleus; 13: Karyogamy; 14: Male chromatin disperses in the egg nucleus; 15: Male nucleolus occurs in the egg nucleus; 16: Zygote; 17: Metaphase of zygote mitosis; 18: Two-celled pro-embryo; 19: Two polar nuclei leant each other, sperm nuclei adhere to the nuclear membrane of one of polar nuclei; 20: Male nucleolus occurs in the polar nucleus just now; 21: Male nucleolus increases volume gradually; 22: After triple fusion of sperm and polar nuclei, three nucleoli occur; 23: Primary endosperm nucleus, a big nucleolus in it resulted from the fusion of the three nucleoli; 24: Metaphase of primary endosperm nucleus mitosis; 25: Anaphase of primary endosperm nucleus mitosis; 26: Telophase of primary endosperm nucleusmitosis; 1.x1 200; 2-5.x125; 6.x800; 7-26.x1 000

Process of fertilization Duration/h From pollination to the first division Time /h
Pollen grain germinates 4 Zygote division 32-34
Pollen tube grows in the style 4 Primary endosperm nucleus division 24
Pollen tube grows in the ovary and enters ovule 6
Pollen tube releases sperms in synergid 2
Two sperms move to egg and polar nuclei 2
Sperm-egg fusion 6
Triple fusion of sperm and polar nuclei 4
Table 1  Duration of each stage of fertilization in Brassica campestris ssp. pekinensis
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