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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2007, Vol. 1 Issue (1) : 90-95     DOI: 10.1007/s11703-007-0017-8
Research article |
Adjuvant effect of an extract from Cochinchina momordica seeds on the immune responses to ovalbumin in mice
Chenwen XIAO,Songhua HU(),Zahid Iqbal Rajput
Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China
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Abstract

The seed of the Momordica cochinchinensis (Lour.) Spreng as a traditional Chinese medicine has been utilized in China for more than 1 200 years. It is traditionally used for the treatment of inflammatory swelling, scrofula, tinea, diarrhea as well as suppurative skin infections such as sore, carbuncles, furuncles and boils in both humans and animals. In this study, an extract from Cochinchina momordica seeds (ECMS) is evaluated for its adjuvant effect and safety. The results suggest that when co-administered with ECMS in Balb/c mice, ovalbumin (OVA, 10 μg) may induce significantly higher specific antibody production than OVA used alone (P<0.05). Analysis of antibody isotypes indicates that the ECMS can promote the production of both IgG1 and IgG2a, but favor the IgG2a. Splenocyte proliferative responses to concanavalin A, lipopolysaccharides or OVA are significantly higher in mice immunized with OVA mixed with ECMS than immunized with OVA alone or mixed with aluminum hydroxide (P<0.05). No local reactions and negative effects on the body weight gain occurred after the injection of OVA mixed with various amounts of ECMS in mice. Therefore, the ECMS is safe for injection and can be used as a potential vaccine adjuvant biasing the production of IgG2a in mice.

Keywords extract of Cochinchina momordica seed      adjuvant      Momordica cochinchinensis (Lour.) Spreng     
Issue Date: 22 February 2016
 Cite this article:   
Chenwen XIAO,Songhua HU,Zahid Iqbal Rajput. Adjuvant effect of an extract from Cochinchina momordica seeds on the immune responses to ovalbumin in mice[J]. Front. Agric. China, 2007, 1(1): 90-95.
 URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-007-0017-8
http://academic.hep.com.cn/fag/EN/Y2007/V1/I1/90
Group No. of mice OVA /μg Adjuvant /μg
1 6 10 -
2 6 10 10 ECMS
3 6 10 50 ECMS
4 6 10 100 ECMS
5 6 10 50 Al-gel
Table 1  Immunization protocol of mice with various OVA-ECMS formulations
Fig. 1  Hemolytic activity of ECMS on RBC from swine, sheep and cattle

The RBC suspension was incubated with saline solution containing ECMS or Quil A (QA) at concentrations ranging from 1 μg to 500 μg/mL at 37°C for 1 hour. After that, samples were centrifuged and the OD value of the supernatant was measured at 405 nm.

Group Before immunization* Before booster* 2 weeks after booster*
1 19.7P1.07 21.6P0.96 22.3P1.15
2 20.6P1.55 21.2P1.53 22.0P1.92
3 20.4P0.27 22.2P0.64 23.0P0.56
4 20.5P.83 22.3P0.31 22.8P0.26
5 20.5P0.94 21.7P1.37 22.5P1.37
Table 2  Effects of subcutaneous injection of 10 μL of OVA mixed with 0 (Group 1), 10 μg (Group 2), 50 μg (Group 3), 100 μg (Group 4) of ECMS or 50 μg of Al-gel (Group 5) on the mean body weight (mean ± SD gram) of mice (n =6)
Fig. 2  Adjuvant effect of ECMS and Al-gel on IgG responses to OVA in mice
Fig. 3  Adjuvant effects of ECMS and Al-gel on the subclass IgG responses in mice
Fig. 4  Effects of ECMS and Al-gel on splenocyte proliferative responses to Con A, LPS and OVA in mice
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