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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2007, Vol. 1 Issue (1) : 105-110     DOI: 10.1007/s11703-007-0020-0
Research article |
Cellular immune responses of BALB/c mice induced by intramuscular injection of PRRSV-ORF5 DNA vaccine with different doses
Anchun CHENG1,2,Mingshu WANG1,2,Xiwen CHEN1,2,3(),Nigen XINI2,Wenbo DOU2,Xuemei LI2,Wumei LIU2,Gang WANG2,Pingying ZHANG2
1 Key Laboratory of Animal Disease and Human Health of Sichuan Province, Ya’an 625014, China
2College of Animal Science and Veterinary Medicine, Sichuan Agricultural University, Yaan, Sichuan 625014, China
3Institute of Applied Animal Technology, Mianyang Normal College, Mianyang 621000, China, Key Laboratory for Molecular Biology and Biopharmaceuticals, Mianyang Normal College, Mianyang, Sichuan 621000, China , The first three authors contribute equally to this work.
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Abstract

BALB/c mice were immunized with 50 µg, 100 µg, 200 µg of pcDNA-PRRSV-ORF5 DNA vaccine respectively by intramuscular injection, with PBS and pcDNA3.1(+) as controls. Fluorescence activated cell Sorter (FACS) was used to detect the number of CD+4 and CD+8 T-lymphocytes. T-lymphocyte proliferation test was used to detect proliferation of the T-lymphocyte cells in peripheral blood lymphocytes of mice vaccinated with pcDNA-PRRSV-ORF5 DNA vaccine. The results showed that the difference in ConA response to T-lymphocytes in blood was highly significant between all experimental groups and the control group (P<0.01). The number of CD4+ T-lymphocytes in experimental groups was significantly higher than that of the control group 7 d after vaccination. The number of CD8+ T-lymphocytes in the experimental groups was higher than that of the control group 28 d after vaccination. Mice immunized with a higher dose (200 µg) of DNA vaccine demonstrated higher cellular immune response than those immunized with a lower dose (100 µg, 50 µg) of DNA vaccine. The results demonstrated that pcDNA-PRRSV-ORF5 DNA vaccine could induce a good cellular immune response which may be dose-dependent.

Keywords PRRSV      ORF5 DNA vaccine      cellular immune response      mice     
Issue Date: 22 February 2016
 Cite this article:   
Anchun CHENG,Mingshu WANG,Xiwen CHEN, et al. Cellular immune responses of BALB/c mice induced by intramuscular injection of PRRSV-ORF5 DNA vaccine with different doses[J]. Front. Agric. China, 2007, 1(1): 105-110.
 URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-007-0020-0
http://academic.hep.com.cn/fag/EN/Y2007/V1/I1/105
Fig. 1  Changes of T-lymphocyte proliferation test OD490 value after inoculation at different doses in BALB/c mice
Fig. 2  Changes of CD 4+ in the peripheral blood of BALB/c mice after inoculation at different doses
Fig. 3  Changes of CD8+ in the peripheral blood of BALB/c mice after inoculation at different doses
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