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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2007, Vol. 1 Issue (3) : 265-270     DOI: 10.1007/s11703-007-0045-4
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Improving organic phosphate utilization in transgenic white clover by overexpression of Aspergillus niger PhyA gene
HAN Shengfang1, GU Juntao1, XIAO Kai2
1.College of Life Science, Agricultural University of Hebei, Baoding 071001, China; College of Agronomy, Agricultural University of Hebei, Baoding 071001, China; 2.College of Agronomy, Agricultural University of Hebei, Baoding 071001, China;
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Abstract Using the cotyledon of white clover as explants, the transgenic white clover lines ectopic expression of the PhyA gene were established based on Agrobacterium tumefaciens-mediated transformation method. It was found that the tested transgenic lines were all Polymerase Chain Reaction (PCR) positive. The transgenic lines 1 to 4 were used for further Southern blot and Northern blot analysis. The lines 1 and 3 with higher level of PhyA expression were used to assay the phytase activities in root and its intercellular space. When the phytate was the sole phosphorus source, the phytase activities in root in lines 1 and 3 were 31.43% and 44.76% higher than those in control (CK), respectively. Meanwhile, the phytase activities in the root intercellular space in lines 1 and 3 were 3.3-fold and 5.12-fold higher than those in CK, respectively. The phosphorus concentration of plants, the accumulative P amount per plant, plant fresh weight, and plant dry weight were all much higher in lines 1 and 3 than in CK. Thus, it is clearly shown that ectopic expression of Aspergillus niger PhyA gene could significantly increase the ability for white clover to utilize organic phosphate under inorganic phosphate (Pi)-deficient condition.
Issue Date: 05 September 2007
 Cite this article:   
HAN Shengfang,GU Juntao,XIAO Kai. Improving organic phosphate utilization in transgenic white clover by overexpression of Aspergillus niger PhyA gene[J]. Front. Agric. China, 2007, 1(3): 265-270.
 URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-007-0045-4
http://academic.hep.com.cn/fag/EN/Y2007/V1/I3/265
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