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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2007, Vol. 1 Issue (3) : 357-360     DOI: 10.1007/s11703-007-0060-5
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Cloning and expression of nucleocapsid protein gene of TGEV HB06 strain
FAN Jinghui1, ZUO Yuzhu1, LI Tanqing1, ZHANG Xiaobo1, ZHAO Yuelan2
1.College of Animal Science and Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China; 2.College of Traditional Chinese Veterinary Medicine, Agricultural University of Hebei, Dingzhou 073000, China
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Abstract The nucleocapsid protein gene of transmissible gastroenteritis virus, 1 149 bp in length, was amplified by RT-PCR from isolated strain HB06 and cloned into pMD18-T. Sequence comparison with other transmissible gastroenteritis virus (TGEV) strains selected from the Gene Bank revealed that the homology of N gene complete sequence shares more than 97% in nucleotide. N gene was cloned into BamHI and EcoRI multiple cloning sites of the prokaryotic expression vector pET 20 b, and named pETN. After being induced by isopropyl-β-D-thiogalactopyranoside (IPTG), the recombinant nucleocapsid protein was expressed. The result of SDS-PAGE and Western-blot showed that the recombinant nucleocapsid protein was 47 kDa and had strong positive reactions with TGEV-specific antibody.
Issue Date: 05 September 2007
 Cite this article:   
FAN Jinghui,ZUO Yuzhu,LI Tanqing, et al. Cloning and expression of nucleocapsid protein gene of TGEV HB06 strain[J]. Front. Agric. China, 2007, 1(3): 357-360.
 URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-007-0060-5
http://academic.hep.com.cn/fag/EN/Y2007/V1/I3/357
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