Study on polymorphisms of microsatellite DNA of six Chinese indigenous sheep and goat breeds

doi:10.1007/s11703-007-0078-8

Front. Agric. China

2007, Vol. 1

Issue (4) : 472-477 https://doi.org/10.1007/s11703-007-0078-8

Study on polymorphisms of microsatellite DNA of six Chinese indigenous sheep and goat breeds

QU Dongyan¹, YANG Zhangping¹, GUO Xiaoya¹, MAO Yongjiang¹, SUN Wei¹, GEN Rongqing¹, REN Xianglian¹, CHANG Guobing¹, HUANG Danli¹, CHANG Hong¹, MA Yuehui²

1.Animal Science and Technology College, Yangzhou University, Yangzhou 225009, China; 2.Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China;

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Abstract The genomes of six populations were screened using microsatellites as molecular markers, including Ujmuqin sheep, small-tailed Han sheep, Tan sheep, Hu sheep, Tong sheep and Yangtse River Delta (YRD) white goat. A total of seven microsatellite markers were used and genetic diversity and genetic distance were also determined. The results showed that there were 224 alleles in six populations, all seven loci showed polymorphism in all populations. The average heterozygosity of all populations was 0.949 9, and the mean polymorphism information content (PIC) of all six populations was 0.842 5–0.929 4. The six sheep (goat) populations were lowly differentiated with all loci, and the coefficient of phaenotype differentiation (Fst) was 2.6%, which was consistent with the coefficient of gene differentiation (Gst). The global heterozygote deficit across of all populations (Fit) amounted to 0.5%. The overall significant deficit of heterozygotes because of inbreeding within breeds (Fis) amounted to -2.2%. Two Unweighted Pair-group Method using Arithmetic Averages (UPGMA) dendrograms were con structed on the basis of Nei s standard genetic distance (DS) and Nei s genetic distance (DA) respectively. Hu sheep and Tong sheep were grouped at first, Ujmuqin sheep and small-tailed Han sheep clustered and then clustered with Tan sheep. Finally, Yangtse River Delta white goat joined in with all above. From this study, Ujmuqin sheep belongs to Mongolia sheep group, which corresponds with the historical records exactly. Ujmuqin sheep and small-tailed Han sheep, Tan sheep, Hu sheep and Tong sheep all vest in the Mongolia sheep group.

Issue Date: 05 December 2007

Cite this article:

YANG Zhangping,QU Dongyan,GUO Xiaoya, et al. Study on polymorphisms of microsatellite DNA of six Chinese indigenous sheep and goat breeds[J]. Front. Agric. China, 2007, 1(4): 472-477.

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https://academic.hep.com.cn/fag/EN/10.1007/s11703-007-0078-8
https://academic.hep.com.cn/fag/EN/Y2007/V1/I4/472

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