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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2008, Vol. 2 Issue (2) : 229-236     DOI: 10.1007/s11703-008-0029-z
Effects of pentoxifylline, platelet activating factor and prostaglandin F 2-alpha on Giant Panda () post-thawed sperm fertilizing capability
HOU Rong1, LAI Songjia2, ZHU Qing2, ZHANG Ming2, XIAN Hong3
1.The Key Laboratory of Reproduction and Conservation Genetic of Endangered Wildlife of Sichuan Province, The Research Base of Giant Panda Breeding; 2.College of Animal Science & Technology, Sichuan Agricultural University; 3.The Department of Human Reproduction and Sterility, Maternity Hospital;
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Abstract Is it possible that Giant Panda (Ailuropoda melanoleuca) post-thawed sperm fertilization is improved with pentoxifylline (PF), platelet activated factor (PAF) and prostaglandin F 2-alpha (PGF2?)? In our study Giant Panda post-thawed sperm was incubated in Ham’s F-10 medium with different concentration of PF, PAF and PGF2? under 37°C. The effects of PF, PAF and PGF2? on Giant Panda post-thawed sperm fertility were evaluated through sperm motility, survival time, sperm membrane integrity, acrosome state and heterospecific egg penetration. The results were that PF, PAF and PGF2? all can affect Giant Panda post-thawed sperm in vitro fertilizing capability. In the experiment: 1 mg·mL-1 PF was most suitable for improving Giant Panda post-thawed sperm in vitro fertilizing capability. In the 1 mg·mL-1 PF group, sperm survival time was (15.33 ± 4.73) h, the heterospecific egg penetration was 51.44% after incubating for 4 hours, the heterospecific egg penetration was 7.49% after incubating for 6 hours. The results of the 1 mg·mL-1 PF group were significantly higher than those of the control group (P < 0.01). The results were also higher than those of the other treatment groups; Treated Giant Panda post-thawed sperm with 50 ng·mL-1 PAF had a better effect than 100 ng·mL-1 PAF, but the sperm fertilizing capability was damaged when incubation time exceeded 2 hours; 50 ng·mL-1 PGF2? had no significant effect on Giant Panda post-thawed sperm, but when the PGF2? treated concentration was increased, sperm in vitro fertilizing capability decreased because of the damaged motility and declined acrosomal reaction rate. The conclusions suggest that it is possible to improve post-thawed Giant Panda sperm fertility with 1 mg·mL-1 PF.
Issue Date: 05 June 2008
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HOU Rong,LAI Songjia,ZHU Qing, et al. Effects of pentoxifylline, platelet activating factor and prostaglandin F 2-alpha on Giant Panda () post-thawed sperm fertilizing capability[J]. Front. Agric. China, 2008, 2(2): 229-236.
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