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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2008, Vol. 2 Issue (4) : 456-462     DOI: 10.1007/s11703-008-0047-x
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Cloning and sequence analysis of a mutation-type cinnamate 4-hydroxylase gene from L. var. DC.
CHEN Anhe1, LI Jiana2, CHAI Yourong2, WANG Rui2, LU Jun2
1.Chongqing Rapeseed Engineering Research Center, Key Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture, College of Agronomy and Biotechnology, Southwest University;College of Bio-information, Chongqing University of Posts and Telecommunications; 2.Chongqing Rapeseed Engineering Research Center, Key Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture, College of Agronomy and Biotechnology, Southwest University;
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Abstract A 2431-bp full-length cinnamate 4-hydroxylase gene, BoC4H, was cloned from Brassica oleracea L. var. acephala DC.. It contains 2 introns. Its mRNA is 1715 bp, encoding a deduced 481-amino-acid polypeptide with wide homologies to C4Hs from other plants. It possesses cytochrome P450 conserved domains and motifs such as the haem-iron binding motif, the E-R-R triad, the T-containing binding pocket motif and the hinge motif necessary for optimal orientation of the enzyme. It also has most of the canonical C4H/CYP73A5-featured substrate-recognition sites (SRSs) and active site residues. However, owing to a single-base deletion at C2242 and subsequent frame shift within the 3′ coding region as compared with C4H genes from Arabidopsis thaliana and other plants, BoC4H shows a 36-aa deletion/variation at its C-terminus and the SRS6 motif together with active site residues therein are absent. Thus BoC4H may be of no function or low activity. BoC4H is a membrane protein and is probably associated with the endoplasmic reticulum. Its secondary structure is dominated by alpha helices and random coils. The Swiss-Model could not predict its tertiary structure. B. oleracea contains a C4H gene family with at least 5 members.
Issue Date: 05 December 2008
 Cite this article:   
LI Jiana,CHEN Anhe,CHAI Yourong, et al. Cloning and sequence analysis of a mutation-type cinnamate 4-hydroxylase gene from L. var. DC.[J]. Front. Agric. China, 2008, 2(4): 456-462.
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http://academic.hep.com.cn/fag/EN/10.1007/s11703-008-0047-x
http://academic.hep.com.cn/fag/EN/Y2008/V2/I4/456
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