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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2008, Vol. 2 Issue (4) : 508-511     DOI: 10.1007/s11703-008-0049-8
Effects of EGF or bFGF on the development of porcine parthenogenetic embryos
LIU Ji1, FENG Shutang2, PAN Dengke2, ZHANG Li2, MU Yulian2, GONG Liguo3, WANG Zirong4
1.Institute of Animal Science, Chinese Academy of Agricultural Sciences;Jinghua Hospital, Shenyang Eastern Medical Group;College of Animal Sciences, Xinjiang Agricultural University; 2.Institute of Animal Science, Chinese Academy of Agricultural Sciences; 3.Jinghua Hospital, Shenyang Eastern Medical Group; 4.College of Animal Sciences, Xinjiang Agricultural University;
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Abstract Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) were added into the culture medium in different culturing stages. The effects of EGF or bFGF on the development of porcine parthenogenetic embryos were studied in vitro. The results were as follows: The addition of EGF significantly enhanced the cleavage rate of porcine parthenogenetic embryos (P < 0.05). The addition of EGF or bFGF also significantly enhanced the rate of blastocysts formation of 2–4-cell porcine parthenogenetic embryos (P < 0.05). Additionally, the group of bFGF had more numbers of blastocysts and higher rates of blastocysts formation than the groups of EGF and the control. In conclusion, EGF and bFGF were found propitious to the development of porcine parthenogenetic embryos in vitro, and bFGF increased the quality of blastocysts by increasing the total cell number in porcine parthenogenetic embryos.
Issue Date: 05 December 2008
 Cite this article:   
FENG Shutang,LIU Ji,PAN Dengke, et al. Effects of EGF or bFGF on the development of porcine parthenogenetic embryos [J]. Front. Agric. China, 2008, 2(4): 508-511.
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