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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2008, Vol. 2 Issue (3) : 253-261     DOI: 10.1007/s11703-008-0052-0
Identification, characterization and expression analysis of transcription factor () genes in rice ( L.)
CAO Yunfei, WANG Jiaojiao, GUO Li, XIAO Kai
College of Agronomy, Agricultural University of Hebei;
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Abstract The acclimation of plants to cold, salt and dehydration is involved in the action of the transcription factor (CBF) cold-response pathway. In this paper, nineteen rice CBF genes, including seven previously released and twelve unpublished novels, were identified and characterized. The multi-members of rice CBFs (OsCBF1 to OsCBF12) were divergent at the nucleotide and amino acid level. Expression analysis shows that five novel rice CBF genes (OsCBF1, OsCBF2, OsCBF3, OsCBF8, and OsCBF9) responded to short-term (1 h or 3 h) stresses of low temperature, salt stress and dehydration. The transcripts of OsCBF2, OsCBF8 and OsCBF9 in the roots were rapidly elevated when the plants were exposed to low temperatures, suggesting that they were possibly involved in low temperature responses in rice plants. Meanwhile, the expression level of OsCBF2 in leaves was enhanced when exposed to salt stress of 1–3 h, implying that OsCBF2 functioned as a transduction component in the salt stress signal cascade. Various expression patterns in OsCBF1, OsCBF2, OsCBF3, OsCBF8, and OsCBF9 under low temperature, salt and drought conditions, together with the different expression patterns between roots and leaves for each of these indicated that every rice CBF gene has unique and non-redundant functions in the response to the abiotic stresses.
Issue Date: 05 September 2008
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CAO Yunfei,WANG Jiaojiao,GUO Li, et al. Identification, characterization and expression analysis of transcription factor () genes in rice ( L.)[J]. Front. Agric. China, 2008, 2(3): 253-261.
1 Artus N N, Uemura M, Steponkus P L, Gilmour S J, Lin C, Thomashow M F (1996). Constitutive expression of the cold-regulated Arabidopsis thaliana COR15a gene affectsboth chloroplast and protoplast freezing tolerance. Proc Natl Acad Sci USA, 93: 13404–13409.
2 Baker S S, Wilhelm K S, Thomashow M F (1994). The 5′ region of Arabidopsis thaliana COR15a has cis-actingelements that confer cold-, drought- and ABA-regulated gene expression. Plant Mol Biol, 24: 701–713.
3 Dorn A, Bollekens J, Staub A, Benoist C, Mathis D (1987). A multiplicityof CCAAT box-binding proteins. Cell, 50: 863–872.
4 Drame K N, Clavel D, Repellin A, Passaquet C, Zuily-Fodil Y (2007). Waterdeficit induces variation in expression of stress-responsive genesin two peanut (Arachis hypogaea L.) cultivars with different tolerance to drought.Plant Physiol Biochem, 45: 236–243.
5 Dubouzet J G, Sakuma Y, Ito Y, Kasuga M, Dubouzet E G, Miura S, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2003). OsDREB genes in rice, Oryzasativa L., encode transcription activators that functionin drought-, high-salt- and cold-responsive gene expression. Plant J, 33(4): 751–763.
6 Gelinas R, Endlich B, Pfeiffer C, Yagi M, Stamatoyannopoulos G (1985). G-substitutionto A-substitution in the distal CCAAT box of the gamma-globin genein Greek hereditary persistence of fetal hemoglobin. Nature, 313: 323–325.
7 Gilmour S J, Sebolt A M, Salazar M P, Everard J D, Thomashow M F (2000). Overexpressionof the Arabidopsis CBF3 transcriptionalactivator mimics multiple biochemical changes associated with coldacclimation. Plant Physiol, 124: 1854–1865.
8 Gilmour S J, Zarka D G, Stockinger E J, Salazar M P, Houghton J M, Thomashow M F (1998). Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptionalactivators as an early step in cold-induced COR gene expression. PlantJ, 16: 433–442.
9 Guarente L, Lalonde B, Gifford P, Alani E (1984). Distinctly regulated tandem upstream activation sites mediate cataboliterepression of the CYC1 gene of S. cerevisiae.Cell, 36: 503–511.
10 Guy C L (1990). Cold acclimation and freezing stress tolerance: roleof protein metabolism. Annu Rev Plant PhysiolPlant Mol Biol, 41: 187–223
11 Haake V, Cook D, Riechmann J L, Pineda O, Thomashow M F, Zhang J Z (2002). Transcription factor CBF4 is a regulatorof drought adaptation in Arabidopsis. Plant Physiol, 130: 639–648.
12 Hahn S, Pinkham J, Wei R, Miller R, Guarente L (1988). The HAP3 regulatorylocus of Saccharomyces cerevisiae encodes divergent overlapping transcripts. Mol Cell Biol, 8: 655–663
13 Jaglo-Ottosen K R, Gilmour S J, Zarka D G, Schabenberger O, Thomashow M F (1998). Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance. Science, 280: 104–106.
14 Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki K (1998). Two transcriptionfactors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separatetwo cellular signal transduction pathways in drought- and low-temperature-responsivegene expression, respectively, in Arabidopsis. Plant Cell, 10: 1391–1406.
15 Ludlow M M, Muchow R C (1990). A criticalevaluation of traits for improving crop yields in water-limited environments. Adv Agron, 43: 107–153.
16 McNabb D S, Xing Y Y, Guarente L (1995). Cloning of yeast HAP5: a novel subunitof a heterotrimeric complex required for CCAAT binding. Genes Dev, 9: 47–58.
17 Medina J, Bargues M, Terol J, Perez-Alonso M, Salinas J (1999). The Arabidopsis CBF gene family is composed ofthree genes encoding AP2 domain-containing proteins whose expressionis regulated by low temperature but not by abscisic acid or dehydration. Plant Physiol, 119: 463–470.
18 Muro A F, Bernath V A, Kornblihtt A R (1992). Interaction of the -170-cyclic AMPresponse element with the adjacent CCAAT box in the human fibronectingene promoter. J Biol Chem, 267: 12767–12774
19 Nakashima K, Tran L S, Van Nguyen D, Fujita M, Maruyama K, Todaka D, Ito Y, Hayashi N, Shinozaki K, Yamaguchi-Shinozaki K (2007). Functional analysis of a NAC-type transcription factor OsNAC6 involvedin abiotic and biotic stress-responsive gene expression in rice. Plant J, 51: 617–630.
20 Nanjo T, Kobayashi M, Yoshiba Y, Kakubari Y, Yamaguchi-Shinozaki K, Shinozaki K (1999). Antisense suppressionof proline degradation improves tolerance to freezing and salinityin Arabidopsis thaliana. FEBS Lett, 461: 205–210.
21 Pinkham J L, Guarente L (1985). Cloningand molecular analysis of the HAP2 locus: a global regulator of respiratorygenes in Saccharomyces cerevisiae. Mol Cell Biol, 5: 3410–3416
22 Pinkham J L, Olesen J T, Guarente L P (1987). Sequence and nuclear localizationof the Saccharomyces cerevisiae HAP2 protein, a transcriptional activator. Mol Cell Biol, 7: 578–585
23 Raymondjean M, Cereghini S, Yaniv M (1988). Several distinct CCAAT box binding-proteinscoexist in eukaryotic cells. Proc NatlAcad Sci USA, 85: 757–761.
24 Redondo-Gomez S, Mateos-Naranjo E, Davy A J, Fernandez-Muno F, Castellanos E M, Luque T, Figueroa M E (2007). Growth and photosynthetic responses to salinity of thesalt-marsh shrub Atriplex portulacoides. Ann Bot, 100: 555–563.
25 Riechmann J L, Meyerowitz E M (1998). The AP2/EREBPfamily of plant transcription factors. Biol Chem, 379: 633–646
26 Rieping M, Schöffl F (1992). Synergisticeffect of upstream sequences, CCAAT box elements, and HSE sequencesfor enhanced expression of chimeric heat-shock genes in transgenictobacco. Mol Gen Genet, 231: 226–232
27 Santoro C, Mermod N, Andrews P C, Tjian R (1988). A family of human CCAAT-box-binding proteins active in transcriptionand DNA replication: cloning and expression of multiple cDNAs. Nature, 334: 218–224.
28 Skinner J S, von Zitzewitz J, Szucs P, Marquez-Cedillo L, Filichkin T, Amundsen K, Stockinger E J, Thomashow M F, Chen T H, Hayes P M (2005). Structural, functional, and phylogenetic characterization of a large CBF gene family in barley. Plant Mol Biol, 59(4): 533–551.
29 Steponkus P L, Uemura M, Joseph R A, Gilmour S J, Thomashow M F (1998). Modeof action of the COR15a gene onthe freezing tolerance of Arabidopsis thaliana. Proc Natl Acad Sci USA, 95: 14570–14575.
30 Stockinger E J, Gilmour S J, Thomashow M F (1997). Arabidopsisthaliana CBF1 encodes an AP2 domain-containing transcriptionalactivator that binds to the C-repeat/DRE, a cis-acting DNA regulatoryelement that stimulates transcription in response to low temperatureand water deficit. Proc Natl Acad Sci USA, 94: 1035–1040.
31 Surjus A, Durand M (1996). Lipidchanges in soybean root membranes in response to salt treatment. J Exp Bot, 47: 17–23.
32 Taji T, Ohsumi C, Iuchi S, Seki M, Kasuga M, Kobayashi M, Yamaguchi-Shinozaki K, Shinozaki K (2002). Important roles ofdrought- and cold-inducible genes for galactinol synthase in stresstolerance in Arabidopsis thaliana. Plant J, 29: 417–426.
33 Thomashow M F (1999). Plant cold acclimation: Freezing tolerance genes andregulatory mechanisms. Annu Rev Plant PhysiolPlant Mol Biol, 50: 571–599.
34 Thomashow M F (2001). So what's new in the field of plant cold acclimation?Lots! Plant Physiol, 125: 89–93.
35 Xiong L, Schumaker K S, Zhu J K (2002). Cell signaling during cold, drought,and salt stress. Plant Cell, 14(Suppl): 165–183
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