Please wait a minute...
Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2008, Vol. 2 Issue (4) : 474-479     DOI: 10.1007/s11703-008-0067-6
|
Association of polymorphisms in adipocyte fatty acid binding protein gene with fat-related traits in chicken
YE Manhong1, CAO Honghe2, LI Hongbin2, CHEN Jilan2, ZHAO Guiping2, ZHENG Maiqing2
1.College of Bioscience and Biotechnology, Yangzhou University; 2.National Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences;
 Download: PDF(165 KB)   HTML
 Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks
Abstract PCR-SSCP analysis was used to detect polymorphic sites in chicken adipocyte fatty acid binding protein (A-FABP) gene. Six Chinese local breeds, Beijing-You chicken, Dwarf chicken, Taihe silky chicken, Chongrenma chicken, Xiayan chicken, Luyuan chicken and an introduced foreign breed, Arbor Acre broiler, were used as test populations. Three PCR-SSCP loci were detected. Statistical results showed that frequencies of genotypes and alleles were significantly different in the test populations. Sequence analysis revealed that C → T, G → A, and C → T transitions were responsible for the polymorphisms. Some fat-related traits such as body weight, content of intramuscular fat (IMF) and percentage of abdominal fat (AFP) were measured in Dwarf chickens and male Beijing-You chickens. We found out that chicken quality was significantly related to different genotypes in these two populations.
Issue Date: 05 December 2008
 Cite this article:   
CAO Honghe,YE Manhong,LI Hongbin, et al. Association of polymorphisms in adipocyte fatty acid binding protein gene with fat-related traits in chicken[J]. Front. Agric. China, 2008, 2(4): 474-479.
 URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-008-0067-6
http://academic.hep.com.cn/fag/EN/Y2008/V2/I4/474
1 Gerbens F, de Koning D J, Harders F L, Meuwissen T H, Janss L L, Groenen M A, Veerkamp J H, van Arendonk J A, te Pas M F (2000). The effect of adipocyte and heartfatty acid-binding protein genes on intramuscular fat and backfatcontent in Meishan crossbred pigs. J AnimSci, 78: 552–559
2 Gerbens F, Jansen A, van Erp A J, Harders F, Meuwissen T H, Rettenberger G, Veerkamp J H, te Pas M F (1998). The adipocytefatty acid-binding protein locus: characterization and associationwith intramuscular fat content in pigs. Mamm Genome, 9: 1022–1026
3 Gerbens F, Rettenberger G, Lenstra J A, Veerkamp J H, te Pas M F W (1997). Characterization,chromosomal localization and genetic variation of the porcine heartfatty acid-binding protein gene. Mamm Genome, 8(5): 328–332.
doi:10.1007/s003359900433
4 Gerbens F, van Erp A J, Harders F L, Verburg F J, Meuwissen T H, Veerkamp J H, te Pas M F (1999). Effect of genetic variants of the heart fatty acid-binding proteingene on intramuscular fat and performance traits in pigs. J Anim Sci, 77: 846–852
5 Gerbens F, Verburg F J, van Moerkerk H T, Engel B, Buist W, Veerkamp J H, te Pas M F (2001). Associationof heart and adipocyte fatty acid-binding protein gene expressionwith intramuscular fat content in pigs. J Anim Sci, 79: 347–354
6 Meng A M, Qi S Z, Gong G F (1993). DNA footprints of poultry developedby four probes. Advancements in Biochemistryand Biophysics, 20(2): 139–142 (in Chinese)
7 Veerkamp J H, Maatman R G (1995). Cytoplasmicfatty acid binding proteins: their structure and genes. Prog Lipid Res, 34: 17–52.
doi:10.1016/0163-7827(94)00005-7
8 Veerkamp J H, Peeters R A, Maatman R G (1991). Structural and functional featuresof different types of cytoplasmic fatty acid-binding proteins. Biochim Biophys Acta, 1081: 1–24
9 Ye M H (2003). Clone of chicken fatty acid binding protein genes andtheir relationship with intramuscular fat content. Dissertation forthe Doctoral Degree. Beijing: Chinese Academy of Agricultural Sciences (in Chinese)
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed