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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2009, Vol. 3 Issue (1) : 104-108     DOI: 10.1007/s11703-008-0080-9
Research Article |
Prophylactic and therapeutic effects of egg yolk immunoglobulin against porcine transmissible gastroenteritis virus in piglets
Yuzhu ZUO1, Jinghui FAN1(), Huixia FAN2, Tanqing LI1, Xiaobo ZHANG1
1. College of Animal Science and Veterinary Medicine, Agricultural University of Hebei; 2. College of Traditional Chinese Veterinary Medicine, Agricultural University of Hebei
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Porcine transmissible gastroenteritis virus (TGEV) is the causative agent of acute diarrhea of newborn piglets that provokes high mortality rates in affected farms. In this study, specific immunoglobulin from egg yolk against TGEV was produced by immunization of White leghorn hens. Enzyme-linked immunosorbent assay (ELISA) and virus neutralization (VN) test revealed that the specific antibody titer started to increase on the tenth day post-immunization, reached its peak on the eighth week, and remained at a high level until the last week that we tested. The prophylactic and therapeutic effects of egg yolk immunoglobulin (IgY) was investigated in piglets. IgY was found effective to increase piglets survival rate significantly after challenge exposures in prophylactic efficacy analysis. The therapeutic effects test revealed that the mortality was dramatically reduced by orally administered IgY. All these results in our study indicated that IgY specific to TGEV could be an alternative prophylactic method like colostral antibodies against TGEV in piglets.

Keywords egg yolk immunoglobulin      immunoprophylactic effect      porcine transmissible gastroenteritis virus     
Corresponding Authors: FAN Jinghui,   
Issue Date: 05 March 2009
URL:     OR
Fig0  Recovery of immunoreactive IgY
Note: A: Egg yolk pooled; B: Crude extract of IgY by water dilution; C: Purified IgY by 40% ammonium sulphate.
treatmentno. of pigletsdays after challenge exposure (no. of survival/head of piglet)
Tab0  Prophylactic effect of IgY in piglets after challenge exposure
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[1] Jinghui FAN, Yuzhu ZUO, Tanqing LI, Xiaobo ZHANG, . Preparation and physicochemical property of chicken yolk immunoglobulin (IgY) against porcine transmissible gastroenteritis virus (TGEV)[J]. Front. Agric. China, 2009, 3(4): 466-470.
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