Please wait a minute...
Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2009, Vol. 3 Issue (1) : 104-108     DOI: 10.1007/s11703-008-0080-9
Research Article |
Prophylactic and therapeutic effects of egg yolk immunoglobulin against porcine transmissible gastroenteritis virus in piglets
Yuzhu ZUO1, Jinghui FAN1(), Huixia FAN2, Tanqing LI1, Xiaobo ZHANG1
1. College of Animal Science and Veterinary Medicine, Agricultural University of Hebei; 2. College of Traditional Chinese Veterinary Medicine, Agricultural University of Hebei
Download: PDF(101 KB)   HTML
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks
Abstract  

Porcine transmissible gastroenteritis virus (TGEV) is the causative agent of acute diarrhea of newborn piglets that provokes high mortality rates in affected farms. In this study, specific immunoglobulin from egg yolk against TGEV was produced by immunization of White leghorn hens. Enzyme-linked immunosorbent assay (ELISA) and virus neutralization (VN) test revealed that the specific antibody titer started to increase on the tenth day post-immunization, reached its peak on the eighth week, and remained at a high level until the last week that we tested. The prophylactic and therapeutic effects of egg yolk immunoglobulin (IgY) was investigated in piglets. IgY was found effective to increase piglets survival rate significantly after challenge exposures in prophylactic efficacy analysis. The therapeutic effects test revealed that the mortality was dramatically reduced by orally administered IgY. All these results in our study indicated that IgY specific to TGEV could be an alternative prophylactic method like colostral antibodies against TGEV in piglets.

Keywords egg yolk immunoglobulin      immunoprophylactic effect      porcine transmissible gastroenteritis virus     
Corresponding Authors: FAN Jinghui,Email:jinghui76@163.com   
Issue Date: 05 March 2009
URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-008-0080-9     OR     http://academic.hep.com.cn/fag/EN/Y2009/V3/I1/104
Fig0  Recovery of immunoreactive IgY
Note: A: Egg yolk pooled; B: Crude extract of IgY by water dilution; C: Purified IgY by 40% ammonium sulphate.
treatmentno. of pigletsdays after challenge exposure (no. of survival/head of piglet)
456781014
control77/75/74/73/73/73/73/7
treatment88/88/87/87/87/87/87/8
Tab0  Prophylactic effect of IgY in piglets after challenge exposure
1 Akita E M, Nakai S (1993). Comparison of four purification methods for the production of immunoglobulins from eggs laid by hens immunized with an enterotoxigenic E. coli strain.J Immunol Methods , 160(2): 207–214
doi: 10.1016/0022-1759(93)90179-B
2 Carlander D, Kollberg H, Wejaker P E, Larsson A (2000). Peroral immunotherapy with yolk antibodies for the prevention and treatment of enteric infections. Immunol Res , 21: 1–6
doi: 10.1385/IR:21:1:1
3 Cox E, Pensaert M B, Callebaut P, van D K (1990). Intestinal replication of a porcine respiratory coronavirus closely related antigenically to the enteric transmissible gastroenteritis virus. Veterinary Microbiology , 23: 237–243
doi: 10.1016/0378-1135(90)90154-N
4 Ding Y H, Ding A Y, Wang Q J, Xu J S (2008). Development egg-yolk antibodies in passive protection of piglet diarrhea caused by enterotoxicgenic E. coli.Chinese Journal of Veterinary Medicine , 44(1): 26–28 (in Chinese)
5 Fan J H, Zuo Y Z, Zhao Y L, Li T Q, Zhang X B (2007). Cloning and expression of nucleocapsid protein gene of TGEV HB06 strain. Front Agric China , 1(3): 357–360
doi: 10.1007/s11703-007-0060-5
6 Hennig-Pauka I, Stelljes I, Waldmann K H (2003). Studies on the effect of specific egg antibodies against Escherichia coli infections in piglets. Dtsch Tierarztl Wochenschr , 110(2): 49–54
7 Kariyawasam S, Wilkie B N, Gyles C L (2004). Resistance of broiler chickens to Escherichia coli respiratory tract infection induced by passively transferred egg-yolk antibodies. Vet Microbiol , 98 (3–4):273–284
doi: 10.1016/j.vetmic.2003.10.022
8 Ko K Y, Ahn D U (2007). Preparation of immunoglobulin Y from egg yolk using ammonium sulfate precipitation and ion exchange chromatography. Poult Sci , 86(2): 400–407
9 Kweon C H, Kwon B J, Woo S R, Kim J M, Woo G H, Son D H, Won H, Lee Y S (2000). Immunoprophylactic effect of chicken egg yolk immunoglobulin (IgY) agaist Porcine Epidemic Diarrhea Virus (PEDV) in piglets. J Vet Med Sci , 62(9): 961–964
doi: 10.1292/jvms.62.961
10 Laude H, Chapsal J M, Gelfi J, Lablau S, Grosclaude J (1986). Antigen structure of transmissible gastroenteritis virus. I. Properties of monoclonal antibodies directed against virion proteins.Journal of General Virology , 67: 119–130
doi: 10.1099/0022-1317-67-1-119
11 Li J T (2003). Egg yolk immunoglobulin (IgY) and its immunoprophylactic effect in piglets. Swine Production , 4: 9–11 (in Chinese)
12 Li X L, Shuai J B, Fang W H (2006). Protection of Carassius auratus Gibelio against infection by Aeromonas hydrophila using specific immunoglobulins from hen egg yolk. J Zhejiang Univ Sci , 7(11): 922–928
doi: 10.1631/jzus.2006.B0922
13 Liu Y, Bai Y D, Lu N, Dong L N, Hu G X (2007). Advances on vaccines of swine transmissible gastroenteritis and porcine epidemic diarrhea. Chinese Journal of Veterinary Drug , 41(2): 40–45
14 Lu Y J, Chi S H, Ma C Q (2007). Advance in drug treatment for transmissible gastroenteritis of swine. Progress in Veterinary Medicine , 28(8): 80–83 (in Chinese)
15 Saif L J, Wesley R D (1999). Transmissible gastroenteritis and porcine respiratory coronavirus. Disease of Swine. 8th ed.Ames: Iowa State University Press, 295–325
16 Sarker S A, Casswall T H, Juneja L R, Hoq E, Hossain I, Fuchs G J, HammarstromL (2001). Randomized, placebo-controlled, clinical trial of hyperimmunized chicken egg yolk immunoglobulin in children with rotavirus diarrhea. J Pediatr Gastroenterol Nutr , 32: 19–25
doi: 10.1097/00005176-200101000-00009
17 Shin J H, Yang M, Nam S W, Kim J T, Myung N H, Bang W G, Roe I H (2002). Use of egg yolk-derived immunoglobulin as an alternative to antibiotic treatment for control of Helicobacter pylori infection. Clinical and Diagnostic Laboratory Immunology , 9(5): 1061–1066
doi: 10.1128/CDLI.9.5.1061-1066.2002
18 Siddell S G, Anderson R, Cavanagh D (1983). Coronaviridae. Intervirology , 20: 181–189
doi: 10.1159/000149390
19 Song W P, Xu F Z, Wang J L, Yang B, Lai P A, Meng Y N (2003). Effect of IgY treatment on porcine epidemic diarrhea virus(PEDV) in piglets. Acta Agriculturae Boreali-Sinica , 218(1): 114–11 (in Chinese)
20 Tuboly T, Nagy E (2001). Construction and characterization of recombinant porcine adenovirus serotype 5 expressing the transmissible gastroenteritis virus spike gene. Journal of General Virology , 82: 183–190
21 Verdolva A, Basile G, Fassina G (2000). Affinity purification of immunoglobulins from chicken egg yolk using a new synthetic ligand. J? Chromatogr? B , 749: 233–242
doi: 10.1016/S0378-4347(00)00426-6
22 Wang X Y, Bao Y M, An L J (2004). Progress in study of egg yolk immunoglobulin. Immunological Journal , 20(Suppl 1): S112–S114 (in Chinese)
23 Yan Q G, Ou Y, Guo W Z, Feng T, Fan W Q, Lai W L, Cao H Z, Li B (2007). Isolation of SC-1strain of transmissible gastroenteritis virus of swine and characteristic analysis of gene7. Chin J Vet Sci , 27(5): 613–616 (in Chinese)
24 Yang F, Zhang L F (2007). The seperation and purification of IgY from egg yolk. Chinese Journal of Comparative Medicine , 17(11): 6–7 (in Chinese)
25 Yang L L, Guo F S, Sun S F, Wu B, Chen H C (2005). Comparsion of antigenicity between expressed proteins of the fragment including S gene whole antigenic sites and the deleted fragment in porcine respiratory coronavirus of transmissible gastroeritis virus. Chinese Journal of Virolog , 21(5): 384–388 (in Chinese)
26 Yokoyama H, Peralta R C, Sendo S, Ikemori Y, Kodama Y (1993). Detection of passage and absorption of chicken egg yolk immunoglobulins in the gastrointestinal tract of pigs by use of enzyme-linked immunosorbent assay and fluorescent antibody testing. Am J Vet Res , 54: 867–872
[1] Jinghui FAN, Yuzhu ZUO, Tanqing LI, Xiaobo ZHANG, . Preparation and physicochemical property of chicken yolk immunoglobulin (IgY) against porcine transmissible gastroenteritis virus (TGEV)[J]. Front. Agric. China, 2009, 3(4): 466-470.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed