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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2009, Vol. 3 Issue (1) : 24-28     DOI: 10.1007/s11703-009-0002-5
RESEARCH ARTICLE |
Cytological observation of the microspore development of Chinese kale and false pakchoi
Zhijun LI(), Yanrong ZHANG, Chunyan LI, Weiping LONG, Wengjia LU, Fuguang HAN
Seed and Seedling Centre, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
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Abstract  

The development process and morphology of the microspores in Chinese kale and false pakchoi were observed by using the whole stain-clearing technique. The results showed that the morphological characteristics of microspores were not only extremely similar, but were also in their development processes. The microspores at tetrad stage showed an arrangement of tetrahedral type, and after being released and passing through two mitoses, they developed gradually to form rotundly-shaped mature pollen grains containing three nuclei, one bigger alimentation nucleus and two smaller similar-sized generative nuclei. Determination of bud sizes at four typical microspore developmental stages revealed that the bud size had a stable heredity at each development stage of the microspores. The ratio of the bud length at the late uninucleate stage to the largest bud length differed little between Chinese kale and false pakchoi, ranging from 0.37 to 0.45 with an average of 0.41, though there was significant difference among their cultivars. It was concluded that the length of buds at late uninucleate stage can be estimated for undetermined cultivars of these two Brassica crops by multiplying the largest bud length with the following coefficient or regression equation: Y = 0.3898X+0.1503, where X is the length of the largest bud.

Keywords Chinese kale      false pakchoi      microspore      late uninucleate stage     
Corresponding Authors: LI Zhijun,Email:lizhijun04@yahoo.com.cn   
Issue Date: 05 March 2009
URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-009-0002-5     OR     http://academic.hep.com.cn/fag/EN/Y2009/V3/I1/24
Fig.1  Microspore developments of Chinese kale and false pakchoi
Note: A-I stand for Cuibao, a Chinese kale cultivar, and J-Q for Sijiyouqing, a false pakchoi cultivar; A, J and K are tetrads; B, C and L are at mid uninucleate stage; D and M are at late uninucleate stage; E and N are in uninucleate mitosis; F and O are at binucleate stage; G and P are in generative nucleus division; H and Q are trinucleate mature pollens; I is the abortive microspore; Bar= 5 μm.
speciescultivarblooming bud numberbud length/mm
the largest bud (trinucleate)binucleatelate uninucleatemid uninucleatetetrad
Chinese kaleCuibao19.30 ± 0.246.58 ± 0.103.43 ± 0.432.70 ± 0.181.94 ± 0.11
59.35 ± 0.246.50 ± 0.163.50 ± 0.412.73 ± 0.171.93 ± 0.10
159.38 ± 0.256.65 ± 0.173.50 ± 0.412.78 ± 0.211.90 ± 0.08
Lubao17.63 ± 0.485.33 ± 0.253.38 ± 0.352.30 ± 0.141.43 ± 0.10
57.55 ± 0.355.38 ± 0.173.28 ± 0.152.38 ± 0.131.48 ± 0.13
157.50 ± 0.255.35 ± 0.213.28 ± 0.382.33 ± 0.241.50 ± 0.08
false pakchoiSijiyouqing14.60 ± 0.242.98 ± 0.331.98 ± 0.131.35 ± 0.130.68 ± 0.10
54.43 ± 0.302.90 ± 0.271.98 ± 0.171.38 ± 0.260.73 ± 0.17
154.73 ± 0.222.95 ± 0.132.03 ± 0.131.40 ± 0.180.70 ± 0.18
Guangfuqing14.05 ± 0.212.38 ± 0.261.58 ± 0.221.00 ± 0.160.50 ± 0.08
54.05 ± 0.102.58 ± 0.101.50 ± 0.080.95 ± 0.130.50 ± 0.08
154.08 ± 0.102.60 ± 0.081.58 ± 0.100.98 ± 0.130.53 ± 0.10
Tab.1  Size of the largest buds and buds at four typical microspore development stages
Fig.2  Correlation between bud length at late uninucleate stage and the largest bud length
Note: ** means significantly correlated at ≤0.01.
Fig.3  Difference in ratios of bud length at late uninucleate stage to the largest bud length among Chinese kale and false pakchoi cultivars
Note: Different letters show significant differences at ≤0.05.
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