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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2009, Vol. 3 Issue (1) : 95-99     DOI: 10.1007/s11703-009-0015-0
RESEARCH ARTICLE |
Protective effects of selenium and vitamin E on rats consuming maize naturally contaminated with mycotoxins
Jie YU, Daiwen CHEN, Bing YU()
Institute of Animal Nutrition, Sichuan Agricultural University, Yaan 625014, China
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Abstract  

Protective effects of antioxidant additives of selenium and vitamin E on rats that consumed maize naturally contaminated with mycotoxins were explored in this paper. Thirty-two Wistar female rats were randomly divided into four groups. The control group was given the basic diet with normal maize. The contaminated maize group was given the diet in which normal maize was replaced by mycotoxin-contaminated maize. The selenium group and vitamin E group were respectively fed mycotoxin-contaminated diet supplemented with 0.4 mg?middot;kg-1 selenium from yeast or 100 mg?middot;kg-1 vitamin E. The trial lasted for 4 weeks. Compared with the control group, antioxidative status was decreased significantly in the contaminated maize group. However, the status in the selenium group and vitamin E group was increased significantly compared with the contaminated maize group. The activities of enzymes related with liver function were significantly higher in the contaminated maize group than those in the control group, whereas they were significantly lower in the selenium group and/or the vitamin E group compared to the contaminated maize group. It is concluded that selenium and vitamin E were able to alleviate oxidative stress and liver function damage due to the consumption of maize naturally contaminated with mycotoxins.

Keywords selenium      vitamin E      mycotoxin-contaminated maize      rats     
Corresponding Authors: YU Bing,Email:ybingtian@yahoo.com.cn   
Issue Date: 05 March 2009
URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-009-0015-0     OR     http://academic.hep.com.cn/fag/EN/Y2009/V3/I1/95
itemsdiet/%
control (T1)contaminated(T2)selenium(T3)vitamin E (T4)
ingredient/%
maize65.20
contaminated maizea65.2065.2065.20
soybean meal27.8627.8627.8427.82
soybean oil2.202.202.202.20
calcium-phosphorus1.701.701.701.70
limestone1.401.401.401.40
L-lysine0.550.550.550.55
DL-methionine0.230.230.230.23
iodized salt0.200.200.200.20
choline chloride0.100.100.100.10
flavour additive0.020.020.020.02
vitamin mixtureb0.040.040.040.04
mineral mixturec0.500.500.500.50
vitamin E0.02
selenium enriched yeast0.04
nutrient composition
digestive energy/MJ?middot;kg-114.1214.1214.1214.11
crude protein /%18.0018.1318.1318.11
crude fibre / %2.492.492.492.49
calcium /%1.001.001.001.00
phosphorus /%0.650.650.650.65
lysine /%1.321.321.321.32
methionine+cystine /%0.790.790.790.79
Tab.1  Composition of experimental diets (as fed-basis)
itemsbasic diet/mg?middot;kg-1mycotoxin-contaminated diet/mg?middot;kg-1
aflatoxinNDND
deoxynivalenol1.101.02
nearalenoneNDND
fumonisin3.503.44
T-2 toxin0.280.45
ochratoxin1.006.10
Tab.2  Mycotoxin content of experimental diets (as fed-basis)
growth measurementsT1/gT2/gT3/gT4/gSEM/g
Body weight gain20.8520.5421.7021.450.54
average daily feed intake21.1020.7821.9821.640.55
Tab.3  Growth measurement of rats
antioxidant statusT1T2T3T4SEM
MDA/nmol?middot;mL-13.173.462.853.500.26
SOD/U?middot;mL-1263.51182.53a267.59b218.14c2.10
GPX/U1573.68542.11a1205.26c1271.05c46.79
T-AOC/U?middot;mL-15.784.506.278.67c0.66
NO/μmol·mL-18.9650.00a7.51b39.31c1.04
Tab.4  The antioxidant status in serum of rats
biochemical measurementsT1T2T3T4SEM
GPT/U?middot;mL-15.7522.61a6.23b7.46b1.95
GOT/U?middot;mL-130.4553.05a24.35b39.24c2.83
LDH/U?middot;L-13110.34821.19a2403.97c3823.40c107.21
ALP/U?middot;100mL-16.087.39a2.15c5.98b0.35
Tab.5  Biochemical measurements of liver functions in serum of rats
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[1] Fei HAN, Daiwen CHEN, Bing YU, Wenli LUO, Daiwen CHEN, Bing YU. Effects of different selenium sources and levels on serum biochemical parameters and tissue selenium retention in rats[J]. Front Agric Chin, 2009, 3(2): 221-225.
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