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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2009, Vol. 3 Issue (2) : 221-225     DOI: 10.1007/s11703-009-0030-1
RESEARCH ARTICLE |
Effects of different selenium sources and levels on serum biochemical parameters and tissue selenium retention in rats
Fei HAN1, Daiwen CHEN1(), Bing YU1, Wenli LUO1, Daiwen CHEN2, Bing YU2
1. Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an 625014, China; 2. Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education of the People’s Republic of China,Ya’an 625014, China
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Abstract  

A total of 54 female Wistar rats were allotted to nine treatments by weight and fed basal diet or diets containing Se of 0.05, 0.10, 0.15, or 0.20 mg?kg-1 diet provided with either Se yeast or sodium selenite for 10 days. The results showed the following: (1) Selenium yeast had better effects compared with sodium selenite on increasing serum superoxide dismutase activities (P<0.05). Addition of Se yeast or sodium selenite increased the activities of serum gluthathione peroxidase (P<0.01); (2) According to slope ratio assay, the bioavailability of Se from Se yeast was 132.1%, 205.7%, 140.0%, and 107.2% of that from sodium selenite when glutathione peroxidase activities and Se contents in serum, kidney, and liver were used as indicators. It is concluded that Se from Se yeast has higher bioavailability than Se from sodium selenite.

Keywords Wistar rats      selenium yeast      serum biochemical parameters      tissue selenium      bioavailability     
Corresponding Authors: CHEN Daiwen,Email:chendwz@sicau.edu.cn   
Issue Date: 05 June 2009
URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-009-0030-1     OR     http://academic.hep.com.cn/fag/EN/Y2009/V3/I2/221
ingredientcontentnutrient levelcontent
corn starch48.30DE /MJ?kg-116.10
casein21.00CP18.70
soybean oil2.00DLys1.54
cellulose1.00DMet0.57
cane sugar23.60Ca0.83
CaCO31.40AP0.31
NaCl0.30
CaHPO40.80
premix*1.50
choline0.10
total100.00
Tab.1  Composition and nutrient level of basal diet (as feed basis, %)
Se source and level/(mg?kg-1)SOD/(U?mL-1)MDA/(nmol?mL-1)GPX/(U)
sodium selenite (SS)
0301.675.85873.68
0.05303.875.19976.60
0.10302.255.561000.79
0.15303.775.80923.32
0.20306.075.001049.80
selenium yeast (SY)
0301.675.85873.68
0.05312.565.251026.88
0.10311.515.61984.19
0.15320.975.121034.78
0.20326.995.011067.19
pooled SE10.510.4740.51
———————probability———————
ANOVA
Se sources (S)0.045*0.6890.215
Se concentration (C)0.5010.3640.002**
S×C0.7760.8940.554
Tab.2  Serum biochemical parameters of Wistar rats fed with different forms of selenium
Se source and added Se/(mg?kg-1)serum/(μg?mL-1)kidney/(mg?kg-1)liver/(mg?kg-1)muscle/(mg?kg-1)
sodium selenite (SS)
00.1890.6380.4460.222
0.050.1970.6560.4940.223
0.100.1970.6760.5100.225
0.150.2100.6780.5090.224
0.200.2220.6820.5150.225
selenium yeast (SY)
00.1890.6380.4460.222
0.050.1970.6650.5050.223
0.100.2250.6800.5110.226
0.150.2220.6810.5110.225
0.200.2580.7070.5250.231
pooled SE0.0030.0140.0240.021
————————probability———————
ANOVA
Se sources (S)0.001**0.3290.7620.909
Se concentration (C)0.001**0.005**0.029*0.999
S×C0.001**0.8860.9991.000
Tab.3  Serum, kidney, liver, and muscle Se concentrations of Wistar rats fed with diets with different dietary concentrations and sources of Se
dependentssodium selenite (SS) Se yeast (SY)RBV(b)/%
interceptSS slopeP-valueinterceptSY slopeP-value
GPX/(U)905.0597.90.035918.4789.80.003132.1
serum Se/(mg?kg-1)0.1870.1570.0010.1860.3230.001205.7
kidney Se/(mg?kg-1)0.6440.2200.0200.6430.3080.009140.0
liver Se/(mg?kg-1)0.4640.3060.0420.4670.3280.046107.2
Tab.4  The relative bioavailability values (RBV) for Se yeast relative to sodium selenite
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