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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2009, Vol. 3 Issue (2) : 178-185     DOI: 10.1007/s11703-009-0031-0
RESEARCH ARTICLE |
Effects of aqueous leaf extracts of P. at different ages on the growth and photosynthetic characteristics
Meiqiu ZHU, Ying WANG, Bingxiang LIU, Lili ZHANG, Hui WANG, Yuxin YUAN, Kejiu DU()
College of Forestry, Agricultural University of Hebei, Baoding 071000, China
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Abstract  

Through the outdoor potted plant trials, the allelopathic potential of Populus tomentosa was tested against its species in the growth, chlorophyll content, and photosynthetic and chlorophyll fluorescence characteristics with aqueous extracts (0.01, 0.02, 0.05, and 0.1 g?mL-1) obtained from leaves at different individual ages (1, 20, and 45 years old). The results showed that seedling height, basal diameter, fresh and dry weights, quantity of chlorophyll, the ratio of chlorophyll a/b, net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), efficiency of primary conversion of light energy of PSII (Fv/Fm), potential activity of PSII (Fv/F0), and photochemical quenching (qP) of the seedlings gradually decreased with the increase of extract concentration of all three ages when compared with the controls. The older the P. tomentosa used for extract preparation, the greater the percentage declined in the aforementioned parameters. Moreover, at the four concentrations used, there was a significant difference between treatments with the extracts from 1- and 45-year-old plants (except for qP), but occasionally, the effects were not obvious between the 1- and the 20-year-old plants, or the 20- and 45-year-old plants. The intercellular CO2 concentration (Ci) treated with the extracts from the 1-year-old decreased at the lowest concentration, whereas it increased at higher concentrations. The Ci treated with aqueous leaf extracts from the 20-year-old decreased at the lower concentrations and increased to similar levels to that of the control at the higher concentrations. Ci was always close to control levels in 45-year-old extract treatments. All the aqueous leaf extracts of P. tomentosa at all ages caused an increase of the initial fluorescence (F0). The older P. tomentosa used for the preparation of aqueous leaf extracts caused a greater percentage decline in F0. The nonphotochemical quenching (qN) increased significantly at lower concentrations of all P. tomentosa extracts, whereas it decreased significantly at higher concentrations. It seemed that aqueous leaf extracts from P. tomentosa were harmful to the photosynthetic structure of its own seedlings, inhibited seedling growth, and led to an eventual decrease of biomass. Extracts from older P. tomentosa leaves had more negative effects on the seedling growth of poplar. The effects on photosynthesis are the more important mechanism of the allelopathy of poplar.

Keywords Populus tomentosa      aqueous leaf extracts      photosynthesis      chlorophyll fluorescence     
Corresponding Authors: DU Kejiu,Email:dukejiu@yahoo.com.cn   
Issue Date: 05 June 2009
URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-009-0031-0     OR     http://academic.hep.com.cn/fag/EN/Y2009/V3/I2/178
Fig.1  Effects of aqueous leaf extracts from on the basal diameter (a) and height (b) of seedlings
Fig.2  Effects of aqueous leaf extracts from on the dry weight (a) and fresh weight (b) of the seedlings of
Note: Different letters above the square column indicate significant difference at the same concentration among different ages (including control) (<0.05).
Fig.3  Effects of aqueous leaf extracts from on the chlorophyll content (a) and the ratio of chlorophyll a/b (b) of
Note: Different letters above the square column indicate significant difference at the same concentration among different ages (including control) (<0.05).
Fig.4  Effects of aqueous leaf extracts of on the photosynthetic characteristics of seedlings
Note: (a), (b), (c), and (d) represent net photosynthetic rate, stomatal conductance, intercellular CO concentration, and transpiration rate, respectively. Different letters above the square column indicate significant difference at the same concentration of different ages (including control) (<0.05).
Fig.5  Effects of aqueous leaf extracts of on the chlorophyll fluorescence parameters of its seedlings
Note: (a), (b), (c), (d) and (e) represent initial fluorescence, efficiency of primary conversion of light energy of PSII, potential activity of PSII, photochemical quenching, and nonphotochemical quenching, respectively. Different letters above the square column indicate significant difference at the same concentration among different ages (including control) (<0.05).
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