Biological characteristics of <italic>Bacillus thuringiensis</italic> strain Bt11 and identification of its <italic>cry</italic>-type genes

doi:10.1007/s11703-009-0040-z

Front Agric Chin

2009, Vol. 3

Issue (2) : 159-163 https://doi.org/10.1007/s11703-009-0040-z

RESEARCH ARTICLE

Biological characteristics of Bacillus thuringiensis strain Bt11 and identification of its cry-type genes

Tinghui LIU¹, Wei GUO^1,2(

), Weiming SUN², Yongxiang SUN²

1. College of Plant Protection, Agricultural University of Hebei, Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, Baoding 071001, China; 2. College of Life Sciences, Agricultural University of Hebei, Baoding 071001, China

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Abstract

A novel strain of Bacillus thuringiensis Bt11, isolated from soil samples in China, was classified and characterized in terms of its crystal proteins, cry genes content. The Bt11 strain showed high toxicity against Spodoptera exigua and Helicoverpa armigera neonates. Bt11 strain shares morphological and biochemical characteristics with the previously described Bacillus thuringiensis subsp. kurstaki. SDS-polyacrylamide gel electrophoresis revealed that crystals were composed of several polypeptides ranging from 20 to 130 kDa, of which the 35, 80, and 130 kDa proteins were the major components. PCR-RFLP with total DNA from strain Bt11 and specific primers for cry1, cry2, cry3, cry4/10, cry7, cry8, cry9, and cry11 genes revealed that cry1Aa, cry1Ab, cry1Ia, and cry9Ea genes were present.

Keywords Bacillus thuringiensis insecticidal activity biochemical reaction PCR-RFLP

Corresponding Author(s): GUO Wei,Email:guowei@hebau.edu.cn

Issue Date: 05 June 2009

Cite this article:

Wei GUO,Weiming SUN,Yongxiang SUN, et al. Biological characteristics of Bacillus thuringiensis strain Bt11 and identification of its cry-type genes[J]. Front Agric Chin, 2009, 3(2): 159-163.

URL:

https://academic.hep.com.cn/fag/EN/10.1007/s11703-009-0040-z
https://academic.hep.com.cn/fag/EN/Y2009/V3/I2/159

Tab.1 Bioassay results of Bt11 strain

Fig.1 The spore and crystal of Bt11 strain

Tab.2 Physiological and biochemical characteristics of Bt11 strain

Fig.2 The growth curve of Bt11 strain and standard strain HD-73

Fig.3 SDS-PAGE analysis of isolate Bt11
Note: 1 represents protein molecular weight marker (High) (212, 116, 97, 66, 44 kDa); 2 represents Bt11 strain.

Tab.3 -type gene contents of the isolate Bt11

Fig.4 Garose gel eletrophoresis of PCR-RFLP
Note: (a) represents the result of Bt11 and gene PCR-RFLP, with M represents ΦX174- III digest DNA Maker; 1, 2, 3, 4, 5 and 6 representative of Bt11-K2PCR/ I & I, Bt11-K2PCR, Bt11-K3PCR /R I & I, Bt11-K3PCR, Bt11-S2PCR/c II & I and Bt11-S2PCR, respectively. (b) represents the result of Bt11gene PCR-RFLP with M, 1 and 2 representing 100 bp DNA marker, Bt11-S1iPCR/119 I & I and Bt11-S1Ipcr, respectively. (c) represents the result of Bt11 gene PCR-RFLP with M, 1 and 2 representing DNA marker DL2000, Bt11-S9PCR and Bt11-S9PCR/ I & I, respectively.

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