Please wait a minute...
Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2009, Vol. 3 Issue (2) : 171-177     DOI: 10.1007/s11703-009-0043-9
Mechanism of herbicidal action of the component I from Pythium aphanidermatum
Jiao XU1, Wenchao XU1, Lihui ZHANG1, <NativeName DisplayOrder="Western" Language="chs"><GivenName>Chuan</GivenName><FamilyName>LI</FamilyName></NativeName>1()
1. College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China; 2. Journal Editorial Department of Agricultural University of Hebei, Baoding 071001, China
Download: PDF(111 KB)   HTML
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks

The herbicidal mechanism of the components extracted from Pythium aphanidermatum was examined in this study. Component I was isolated using the HPD500 macroporous adsorption resin and HPLC. Its impact on seed germination and plant growth of weeds was determined and the contents of MDA, superoxide anion radical, and the activities of hills and roots were examined. The root length of weed plants was inhibited under illumination while the stem height was inhibited evidently under darkness. The relative electric conductivity of Digitaria sanguinalis and Amaranthus retroflexus under illumination was 94.55 μS·cm-1 and 58.75 μS?cm-1, respectively, whereas that under darkness was 85.25 μS?cm-1 and 36.25 μS?cm-1, respectively. The MDA contents of Digitaria sanguinalis and Chlorella pyrenoidosa were 0.08385 μmol·L-1 and 0.1742 μmol?L-1 under illumination, respectively, while those were 0.0129 μmol?L-1 and 0.01935 μmol?L-1 under darkness, respectively. Simultaneously, superoxide anion radical content was higher under illumination than under darkness. These results showed the photosynthesis was affected by component I extracted from Pythium aphanidermatum.

Keywords Pythium aphanidermatum      herbicidal component      herbicidal mechanism     
Corresponding Authors: null,   
Issue Date: 05 June 2009
URL:     OR
Fig.1  The HPLC chart of 10% ethanol elution HPD500
item3700 lxdarkness
component Itreatmentwatertreatmentinhibition rate/%component Itreatmentwater treatmentinhibition rate/%
D. sanguinalisroot length/mm44.2649.079.8035.1535.030
stem length/mm13.5214.506.7830.5042.7028.57
A. retroflexusroot length/mm2.5014.8883.222.519.6373.86
stem length/mm8.648.64010.7918.3741.27
Tab.1  Effect of component I on the inhibition of seed germination and plant growth
itemcomponent I treatmentwater treatmentcomponent I contrastwatercontrastrelative electric conductivity
the electric conductivity of D. sanguinalis/(μS?cm-1)natural light (50300-103500 lx)248.0051.20112.009.7594.55
the electric conductivity of A. retroflexus /(μS?cm-1)natural light (50300-103500 lx)189.0028.00112.009.7558.75
Tab.2  The effect of component I on the electric conductivity of and under different light intensities
itemcomponent I treatmentwater treatmentrelative loss/%
the chlorophyll content of C. pyrenoidosa/(μg?mL-1)natural light (59000-89500 lx)0.636.6790.59
Tab.3  Effects of component I on the chlorophyll content of under different light intensities
itemcomponent I treatment/(μmolO2?mg-1chl?h-1)water treatment/(μmolO2?mg-1chl?h-1)relative loss/%
Hill activity ofC. pyrenoidosanatural light(59000-89500 lx)7.5459.8388.19
Hill activity ofD. sanguinalisnatural light(50300-103500 lx)262.03614.1857.33
Tab.4  Effects of component I on Hill activity under different light intensities
itemcomponent I treatmentcontrast
the MDA content ofC. pyrenoidosa/(μmol?L-1)natural light(59000-89500 lx)0.08380.0451
the MDA content ofD. sanguinalis/(μmol?L-1)natural light(50300-103500 lx)0.17420
Tab.5  Effects of component I on the lipid peroxidation under different light intensities
itemcomponent I treatmentcontrast
the superoxide anion radical content of C. pyrenoidosa/(g?min-1)natural light(59000-89500 lx)0.150.04
the superoxide anion radical content of D. sanguinalis/(g?min-1)natural light(50300-103500 lx)0.710
Tab.6  Effects of component I on the superoxide anion radical content under different light intensities
1 Arnon D I (1949). Copper enzymes in isolated chloroplast polyphenoloxidase in Beta vulgaris. Plant Physiology , 24(1): 1-15
doi: 10.1104/pp.24.1.1
2 Chen W, Xue Z X (1995). Research and Development of New Pesticides. Beijing: Chemical Industry Press, 16-65 (in Chinese)
3 Crossmann K, Berghaus R, Petzlaff G (1992). Heterotrophic plant cell suspension cultures for monitoring biological activity in agrochemical research. Pesticide Science , 35(3): 283-289
doi: 10.1002/ps.2780350314
4 Delorenzo M E, Scott G I, Ross P E (2001). Toxicity of pesticides to aquatic microorganisms. Environmental Toxicology and Chemistry , 20(1): 84-98
doi: 10.1897/1551-5028(2001)020&lt;0084:TOPTAM&gt;2.0.CO;2
5 Dong J G, Fan M Z, Han J M, Liu S H, Cao L H (1999). The effect of AB toxin from Alternaria brassicae on membrane permeability and the activities of SOD and POD in cell of Chinese cabbage leaves. Acta Phytopathologica Sinica , 29(2): 138-141 (in Chinese)
6 Huang K (2007). Study on methods and mechanism for removal nitrogen and phosphorus in wastewater by algae. Dissertation for the Masteral Degree . Nanchang: Nanchang University, 17-19 (in Chinese)
7 Jiang S J, Qiang S, Zhu Y Z (2006). Isolation, purification, identification, and bioassay of helminthosporin with herbicidal activity from Curvularia eragrostidis. Journal of Plant Protection , 33(3): 313-318 (in Chinese)
8 Mallory S C A, Retzinger E J (2003). Revised classification of herbicides by site of action for weed resistance management strategies. Weed Technology , 17(3): 605-619
doi: 10.1614/0890-037X(2003)017[0605:RCOHBS]2.0.CO;2
9 Peng H, He H W (2003). The advances in research of inhibitor of carotenoid biosynthesis. Chinese Journal of Pesticide Science , 5(4): 1-8 (in Chinese)
10 Shen G X, Yan G A, Peng J L, Yan X (1999). Study on ecotoxicology for pesticides to algae II: toxic mechanism and accumulation, degradation. Techniques and Equipment for Enviromental Pollution Control , 7(6): 131-139 (in Chinese)
11 Su S Q (1998). Classification of herbicides by target of action and herbicides use. Pesticides , 37(11): 1-7 (in Chinese)
12 Tang Z C, Wei J M, Chen Y (2004). Modern Guide to Plant Physiology Experiments. Beijing: Science Press, 107-109 (in Chinese)
13 Wakabayashi K, Boger P (2002). Target sites for herbicides: entering the 21st century. Pest Management Science , 58(11): 1149-1154
doi: 10.1002/ps.560
14 Wang A G, Luo G H (1990). Quantitative relation between the reaction of hydroxylamine and superoxidee anion radicals in plants. Plant Physiology Communications , 26(6): 55-57 (in Chinese)
15 Wu Y C, Hu F Z, Yang H Z (2001). Research development on the inhibitors of 4-hydroxyphenylpyruvate dioxygenase. Chinese Journal of Pesticide Science , (3): 1-10 (in Chinese)
16 Zhang J L (2005). Structural identification of herbicidal toxin fractions produced and DDRT analysis of mutant isolates of Pythium aphanidermatum. Dissertation for the Doctoral Degree . Baoding: Agricultural University of Hebei, 18 (in Chinese)
17 Zhang J L, Xu K, Li C, Ma J, Dong J G (2005). The bioactivity of mutant isolates from Botrytis cinerea. Scientia Agricultura Sinica , 38(6): 1174-1181 (in Chinese)
18 Zhang R S (1999). Recent herbicide targets and new challenges. World Pesticide , 21(1): 60-61 (in Chinese)
19 Zhao S J (1999). Modern Guide to Plant Physiology Experiments. Beijing: Science Press, 305-306 (in Chinese)
20 Zhou Y H, Miao W R, Cheng L B (2002). Progress on protoporphyrinogen oxidase-inhibiting herbicides. Chinese Journal of Pesticide Science , 4(1): 1-8 (in Chinese)
No related articles found!
Full text