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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2009, Vol. 3 Issue (4) : 446-451     DOI: 10.1007/s11703-009-0068-0
Research articles |
Comparative study on antibiogram of Vibrio spp. isolated from diseased postlarval and marketable-sized white leg shrimp ( Litopenaeus vannamei )
Seong Wei LEE,Musa NAJIAH,Wee WENDY,Musa NADIRAH,
Department of Fisheries Science and Aquaculture, Faculty of Agrotechnology and Food Science, Universiti Malaysia Terengganu, Kuala Terengganu 21030, Malaysia;
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Abstract  This paper compared antibiotic sensitivity between Vibrio spp. isolated from diseased postlarval and marketable-sized white leg shrimp (Litopenaeus vannamei). Recently, white leg shrimp become target species of shrimp culture among shrimp farmers in Malaysia to replace tiger shrimp (Penaeus monodon) culture. However, the baseline information on antibiogram of pathogenic bacteria especially Vibrio spp., the causative agent of vibriosis in white leg shrimp culture is not well established. Therefore, this study was conducted to reveal the antibiogram of Vibrio spp. isolated from diseased postlarval and marketable-sized white leg shrimp. The information gained from this study is useful for shrimp farmers in selecting appropriate antibiotic during disease outbreak. Antibiogram of present bacterial isolates was determined through disk diffusion method against 21 antibiotics (oxolinic acid 2µg, ampicillin 10µg, erythromycin 15µg, furazolidone 15µg, lincomycin 15µg, oleandomycin 15µg, amoxicillin 25µg, colistin sulphate 25µg, sulphamethoxazole 25µg, chloramphenicol 30µg, doxycycline 30µg, florfenicol 30µg, flumequine 30µg, kanamycin 30µg, nalidixic acid 30µg, novobiocin 30µg, oxytetracycline 30µg, tetracycline 30µg, nitrofurantoin 50µg, fosfomycin 50µg, and spiramycin 100µg). A total of 47 Vibrio spp. isolates (Vibrio parahaemolyticus, 24, and V. alginolyticus, 23) from postlarval white leg shrimp and 49 Vibrio spp. isolates (Vibrio parahaemolyticus, 13, Vibrio
Keywords antibiogram      Vibrio spp      white leg shrimp (Litopenaeus vannamei)      postlarval      marketable size      
Issue Date: 05 December 2009
URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-009-0068-0     OR     http://academic.hep.com.cn/fag/EN/Y2009/V3/I4/446
Aguirre-Guzman G, Vazquez-Juarez R, Ascencio F (2001). Differencesin the susceptibility of American white shrimp larval substages (Litopenaeus vannamei) to four Vibrio species. Journal of Invertebrate Pathology, 78: 215―219

doi: 10.1006/jipa.2001.5073
Alcaide E, Gil-Sanz C, Sanjuan E, Esteve D, Amaro C, Silveira L (2001). Vibrio harveyicauses disease in seahorse, Hippocampus sp. Journal of Fish Diseases, 24: 311―313

doi: 10.1046/j.1365-2761.2001.00297.x
Bauer A W, Kirby W M M, Sherris J C, Turck M (1966). Antibiotic susceptibility testingby a standardized single disk method. AmericanJournal of Clinical Pathology, 45: 493―496
Clinical and LaboratoryStandards Institute (CLSI) (2006). Performance standards for antimicrobial susceptibility testing; thesixteenth informational supplement, M100-S16. CLSI, Wayne, USA
Diggles B K, Moss G A, Carson J, Anderson C D (2000). Luminous vibrios in rock lobster Jasus verreauxi (Decapoda: Palinuridae) phyllosoma larvaeassociated with infection by Vibrio harveyi. Diseases of Aquatic Organisms, 43: 127―137

doi: 10.3354/dao043127
Holt J G, Krieg N R, Sneath P H A, Staley J T, Williams S T (1994). Bergey’s Manual of Determinative Bacteriology, Version 9, William & Wilkins, Maryland, USA
Karunasagar I, Pai R, Malathi G R, Karunasagar I (1994). Mass mortality of Penaeus monodon larvae due to antibiotic-resistant Vibrio harveyi infection. Aquaculture, 128: 203―209

doi: 10.1016/0044-8486(94)90309-3
Lavilla-Pitogo C R, Baticados M C L, Cruz-Lacierda ER, de la Pena L D (1990). Occurrenceof luminous bacterial disease of Penaeus monodon larvae in the Philippines. Aquaculture, 91: 1―13

doi: 10.1016/0044-8486(90)90173-K
Lee K K, Liu P C, Chuang W H (2002). Pathogenesisof gastroenteritis caused by Vibrio carchariae in cultured marine fish. Marine Biotechnology, 4: 267―277

doi: 10.1007/s10126-002-0018-9
Liu P C, Chuang W H, Lee K K (2003). Infectiousgastroenteritis caused by Vibrio harveyi (V. carchariae) in cultured reddrum, Sciaenops ocellatus. Journal of Applied Ichthyology, 19: 59―61

doi: 10.1046/j.1439-0426.2003.00356.x
Liu P C, Lee K K, Chen S N (1996). Isolation of Vibrio harveyi from diseased kuruma prawns Penaeus japonicus. Current Microbiology, 33: 129―132

doi: 10.1007/s002849900087
Liu P C, Lee K K, Chen S N (1997). Susceptibility ofdifferent isolates of Vibrio harveyi to antibiotics. Microbios, 91: 175―180
Liu P C, Lin J Y, Chuang W H, Lee K K (2004). Isolation and characterization of pathogenic Vibrio harveyi (Vibriocarchariae) from the farmed marine cobia fish Rachycentron canadum L. with gastroenteritissyndrome. World Journal of Microbiologyand Biotechnology, 20: 495―499

doi: 10.1023/B:WIBI.0000040402.44340.0e
Molina-Aja A, Garcia-Gasca A, Abreu-Grobois A, Bolan-Mejia C, Rogue A, Gomez-Gil B(2002). Plasmidprofiling and antibiotic resistance of Vibrio strains isolated from cultured penaeid shrimp. FEMS Microbiology Letters, 213: 7―12

doi: 10.1111/j.1574-6968.2002.tb11278.x
Najiah M, Lee S W (2008). Outbreak of vibrios in mantis shrimp (Squilla sp.). World Journal of Agricultural Sciences, 4(2): 137―139
Najiah M, Lee S W, Wendy W (2008). Phenotypic and genotypiccharacteristics of Vibrio harveyi isolated from black tiger shrimp (Penaeusmonodon). World Applied SciencesJournal, 3(6): 885―902
Nakayama T, Ito E, Nomura N, Nomura N, Matsumura M (2006). Comparisonof Vibrio harveyi strains isolatedfrom shrimp farms and from culture collection in terms of toxicityand antibiotic resistance. FEMS MicrobiologyLetters, 258: 194―199

doi: 10.1111/j.1574-6968.2006.00225.x
Nicolas J L, Basuyaux O, Mazurie J, Thebault A (2002). Vibrio carcharia, a pathogenof the abalone Haliotis tuberculata. Diseases of Aquatic Organisms, 50: 35―43

doi: 10.3354/dao050035
Nishimori E, Hasegawa O, Numata T, Wakabayashi H (1998). Vibrio carcharia causes massmortality in Japanese abalone, Sulculus diversicolorsupratexta. Fish Pathology (Tokyo), 33: 495―502
Pass D A, Dybdahl R, Mannion M M (1987). Investigationsinto the causes of mortality in the pearl oyster, Pinctata maxima (Jamson), in Western Australia. Aquaculture, 65: 149―169

doi: 10.1016/0044-8486(87)90259-6
Sarter S, Nguyen H N K, Hung L T, Lazard J, Montet D (2007). Antibiotic resistance in Gram-negative bacteria isolatedfrom farmed catfish. Food Control, 18: 1391―1396

doi: 10.1016/j.foodcont.2006.10.003
Soffientino B, Gwaltney T, Nelson D R, Specker J L, Mauel M, Gómez-Chiarri M (1999). Infectiousnecrotizing enteritis and mortality caused by Vibrio carchariae in summer flounder Paralichthys dentatus during intensive culture. Diseases of Aquatic Organisms, 38: 201―210

doi: 10.3354/dao038201
Soto-Rodriguez S A, Roque A, Lizarraga-Partida M L, Guerra-Flores A L, Gomez-Gill B (2003). Virulence of luminous vibrios to Artemia franciscana nauplii. Diseases of Aquatic Organisms, 53: 231―240

doi: 10.3354/dao053231
Tendencia E, de la Pena L D (2001). Antibiotic resistance of bacteria from shrimp ponds. Aquaculture, 195: 193―204

doi: 10.1016/S0044-8486(00)00570-6
Teo J W P, Suwanto A, Poh C L (2000). Novelbeta lactamase genes from two environmental isolates of Vibrio harveyi. Antimicrobial Agents Chemotherapy, 44: 1309―1314

doi: 10.1128/AAC.44.5.1309-1314.2000
Teo J W P, Tan T M C, Poh C L (2002). Genetic determinantsof tetracycline resistance in Vibrio harveyi. Antimicrobial Agents Chemotherapy, 46: 1038―1045

doi: 10.1128/AAC.46.4.1038-1045.2002
Wurmann C, Madrid, R M, Brugger A M (2004). Shrimpfarming in Latin America: currents status, opportunities, challengesand strategies for sustainable development. Aquaculture Economic and Management, 8: 117―141

doi: 10.1080/13657300409380358
Zhang X H, Austin B (2000). Pathogenicity of Vibrio harveyi to salmonids. Journal of Fish Diseases, 23: 93―102

doi: 10.1046/j.1365-2761.2000.00214.x
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