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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2010, Vol. 4 Issue (1) : 18-23     DOI: 10.1007/s11703-009-0080-4
Research articles |
Identification of Lr24 with targeted region amplified polymorphism (TRAP) analysis in wheat
Na ZHANG1,Wenxiang YANG1,Daqun LIU1,Shengliang YUAN2,
1.College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China; 2.Agricultural University of Hebei, Baoding 071001, China;
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Abstract  This research is aimed at developing TRAP markers, as a probe for library screening, closely linked to or co-segregated with Lr24. Ninety TRAP primer pairs were used to test the resistant and susceptible parents, as well as the resistant bulk and the susceptible bulk in our study. The polymorphic TRAP primers of TcLr24 were employed to genotype the F2 population from TcLr24×Thatcher subsequently. Ten of 90 TRAP primer pairs displayed polymorphism between TcLr24 and Thatcher, accounting for 11.11%. A further study found that primer ARBI1/RGA-2F generated a 161bp fragment presented only in the resistance plants of F2 population. Forty-five other wheat leaf rust resistant NILs and 30 diploid materials of wheat were also tested to detect the specificity of the primer. This specific band was amplified in TcLr19, TcLr29, TcLr38, TcLr42 and TcLr44, but absented in all the 30 diploid materials. It was concluded that this marker ARBI1/RGA-2F was closely linked to Lr24, which could be used to detect Lr24 in the F2 population of TcLr24×Thatcher, and be further used as a probe for cDNA and BAC library screening of TcLr24.
Keywords resistance genes      TRAP      TcLr24      wheat leaf rust      
Issue Date: 05 March 2010
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