Detection of indigenous endophytic bacteria in Eucalyptus urophylla in vitro conditions

doi:10.1007/s11703-009-0090-2

Front. Agric. China

2010, Vol. 4

Issue (1) : 37-41 https://doi.org/10.1007/s11703-009-0090-2

Research articles

Detection of indigenous endophytic bacteria in Eucalyptus urophylla in vitro conditions

Hongmiao SHEN¹,Dangyue HAN¹,Fenghuan YANG¹,Qiuxian HUANG¹,Longxian RAN¹,Zhengnan LI²,

1.Section Forest Pathology, College of Forestry, Agricultural University of Hebei, Baoding 071001, China; 2.Mountain Area Research Institute of Hebei Province, Agricultural University of Hebei, Baoding 071001, China;

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Abstract The presence of indigenous endophytic bacteria in aseptically grown seedlings of Eucalyptus urophylla germinated from surface sterilized seeds was investigated using dilution plating, microscopy, and PCR detection. No culturable endophytic bacteria could be detected in suspensions of ground plant tissue incubated on solid or in liquid cultivation media. However, a large number of endophytic bacterial cells, mostly rod-shaped and measured 2–3μm×0.5–0.8μm, were observed in in vitro cultured seedlings of E. urophylla using both light and electron microscopy. Using the universal bacterial 16S rDNA primers, a predicted 190-bp fragment was amplified from total DNA isolated from the seedlings of E. urophylla. We concluded that the endophytic bacteria originated from the seed were present in seedlings of E. urophylla. However, the bacterial cells observed appeared to be nonculturable.

Keywords electron microscopy endophytic bacteria Eucalyptus urophylla PCR

Issue Date: 05 March 2010

Cite this article:

Hongmiao SHEN,Dangyue HAN,Fenghuan YANG, et al. Detection of indigenous endophytic bacteria in Eucalyptus urophylla in vitro conditions[J]. Front. Agric. China, 2010, 4(1): 37-41.

URL:

https://academic.hep.com.cn/fag/EN/10.1007/s11703-009-0090-2
https://academic.hep.com.cn/fag/EN/Y2010/V4/I1/37

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