|
|
|
Salicylic acid induces the accumulation of defense-related
enzymes in Whangkeumbae pear and protects from pear black spot |
| Yongbo WANG,Yuxing ZHANG, |
| College of Horticulture,
Agricultural University of Hebei, Baoding 071001, China; |
|
|
|
|
Abstract The ability of salicylic acid (SA) to induce disease resistance was studied with Whangkeumbae pears affected by Alternaria kikuchiana Tanaka. SA (0.02, 0.2 and 2 mmol·L−1 active ingredient) protected Whangkeumbae pear leaves from artificial infection when applied before inoculation. When the concentration of SA reached 0.2 mmol·L−1, the disease-infected index was the lowest, and the rate of induced resistance reached 59.0%. The protection of Whangkeumbae pear leaves was associated with the activation of three defense-related enzymes, SOD, POD and PAL. Accumulation of three enzymes was induced locally in treated leaves and systemically. These results suggested that SA could induce systemic resistance in Whangkeumbae pear leaves by increasing defense-related compounds.
|
| Keywords
Whangkeumbae pear
Alternaria kikuchiana Tanaka
salicylic acid
induced resistance
|
|
Issue Date: 05 June 2010
|
|
|
Brisset M N, Cesbron S, Thomson S V, Paulin J P (2000). Acibenzolar-S-methyl induces the accumulation of defense-related enzymes in apple and protects fromfire blight. Eur J Plant Pathol, 106: 529–536
doi: 10.1023/A:1008728119087
|
|
Fang Z D (1998). Research Method of Plant Disease. 3rd ed. Beijing: China Agriculture Press, 336–368 (in Chinese)
|
|
Gu Y X, Wang D J (2003). Effect of external salicylic acid on Curvularia lunate Boed on Festuca arundinacea Schreb. Grassland of China, 25(4): 56–60 (in Chinese)
|
|
Hou H, Zhu J L, Zhou H P, Xu H X (2006). Studies on the resistance induced by BTH and SA against Erysiphe cucurbitacearum in muskmelon. Journal of Fruit Science, 23(5): 736–739 (in Chinese)
|
|
Kessmann H, Staub T, Hofmann C, Maetzke T, Herzog J, Ward E, Uknes S, Ryals J (1994). Induction of systemic acquired disease resistance in plants by chemicals. Annu Rev Phytopathol, 32: 439–459
doi: 10.1146/annurev.py.32.090194.002255
|
|
Li Y H, Cheng Z H, Chen P, Chen X G (2006). Studies on induction resistance to Psedoperonospora cubensis by BTH in cucumber seedlings. Acta Horticulturae Sinica, 33(2): 278–282 (in Chinese)
|
|
Liang J G, Zhang B X, Chen Z Y, Yu J Q (2006). Studies on the resistance to cucumber damping-off inducedby plant growth-promoting Rhizobacteria CH1. Acta Horticulture Sinica, 33(2): 283–288 (in Chinese)
|
|
Liu F Q, Wang J S (2000). Preliminary study on resistance of rice seeding to leaf blight inducedby salicylic acid. Acta PhytophylacicaSinica, 27(1): 47–52 (in Chinese)
|
|
Lucas J A (1999). Plant immunisation: from myth toSAR. Pestic Sci, 55: 193–196
doi: 10.1002/(SICI)1096-9063(199902)55:2<193::AID-PS883>3.0.CO;2-5
|
|
Mao A J, Wang Y J, Feng L X, Geng S S, Xu Y (2004). Study on the resistance induced by salicylic acid against Phytophthora capsici in pepper. Scientia Agricultura Sinica, 37(10): 1481–1486 (in Chinese)
|
|
Moerschbacher B M, Noll U M, Flott B E, Reisener H J (1998). Lignin biosynthetic enzymes in stem rust infected, resistance and susceptible nearisogenic wheat lines. Physiological and Molecular Plant Pathology, 33(1): 33–36
doi: 10.1016/0885-5765(88)90041-0
|
|
Mozzetti C, Ferraris L, Tamietti G, Matta A (1995). Variation in enzyme activities in leaves and cell suspension as markers of incompatibility in differentphytophthora-pepper interaction. Physiological and Molecular Plant Pathology, 46: 95–107
doi: 10.1006/pmpp.1995.1008
|
|
Okuno T, Nakyama M, Okajima N, Fursawa I (1991). Systemic resistance to downy mildew and appearance of acid soluble proteins in cucumber leaves treatedwith biotic and abiotic inducers. Annals of the Phytopathological Society of Japan, 57: 203–211
|
|
Olivieri F P, Lobato M C, González Altamiranda E, Daleo G R, Huarte M, Guevara M G, Andreu A B (2009). BABA effects on the behaviour of potato cultivars infected by Phytophthora infestans and Fusarium solani. Eur J Plant Pathol, 123: 47–56
doi: 10.1007/s10658-008-9340-z
|
|
Park S W, Kaimoyo E, Kumar D, Mosher S, Klessig D F (2007). Methyl salicylate is a critical mobile signal for plantsystemic acquired resistance. Science, 318: 112–116
doi: 10.1126/science.1147113
|
|
Rao M V, Paliyath G, Ormrod D P (1996). Ultraviolet-Band ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. Plant Physiology, 110: 125–136
doi: 10.1104/pp.110.1.125
|
|
Rieko H, Kaoru I, Yoshitsugu H, Takayoshi T, Aiko T, Mikihiro Y, Kazuya A, Takashi T (2002). A conditional dispensable chromosome controls host-specific pathogenicity in thefungal plant pathogen Alternaria alternate. Genetics, 161: 59–70
|
|
Shi Q H, Zhu Z J, Xu M, Qian Q Q (2004). Effects of exogenous salicylic acid on activities of some enzymesand antioxidants in cucumber leaves. Acta Horticulture Sinica, 31(5): 666–667 (in Chinese)
|
|
Sparla F, Rotino L, Valgimigli M C, Pupillo P, Trost P (2004). Systemic resistance induced by benzothiadiazole in pear inoculatedwith the agent of fire blight. Scientia Horticulturae, 101: 269–279
doi: 10.1016/j.scienta.2003.11.009
|
|
Wang H, Chang Y H, Chen Z Y (2006). Study on biological characteristics of Alternaria alternata caused occurrence of pear black spot. Journal of Fruit Science, 23(2): 247–251 (in Chinese)
|
|
Wang T, Wang S P, Guo S R, Gao H B (2005). Change of polyamines metabolism in roots of cucumberseedlings under root-zone hypoxia stress. Acta Horticulture Sinica, 32(3): 443–437 (in Chinese)
|
|
Willits M G, Ryals J A (1998). Determining the relationship between salicylic acid levels and systemicacquired resistance induction in tobacco. Molecular Plant-Microbe Interactions, 11: 795–800
doi: 10.1094/MPMI.1998.11.8.795
|
|
Yu D Q, Cen C, Yang M L, Li B J (1999). Tissue variance of salicylic acid-sensitive catalaseand enhancement of disease resistance with exogenous salicylic acidin maize. Acta Botanica Sinica, 41(12): 1293–1298 (in Chinese)
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
