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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2010, Vol. 4 Issue (1) : 10-17     DOI: 10.1007/s11703-010-0094-y
Research articles |
Expression of special genes inhibited by powdery mildew ( Blumeria graminis f. sp. tritici ) in wheat germplasm N9436
Jinhua WU1,Zhiying MA2,Yingang HU3,Hong ZHANG3,Changyou WANG3,Qiuying WANG3,Wanquan JI3,
1.College of Agronomy, Agricultural University of Hebei, Baoding 071001, China;College of Agronomy, Northwest A&F University, Yangling 712100, China; 2.College of Agronomy, Agricultural University of Hebei, Baoding 071001, China; 3.College of Agronomy, Northwest A&F University, Yangling 712100, China;
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Abstract  Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most important fungal diseases in common wheat (Triticum aestivum L.) worldwide. Wheat germplasm N9436 is resistant to powdery mildew. In the present study, a backward subtracted cDNA library was constructed with cDNA from N9436 leaves inoculated by Blumeria graminis as the driver and cDNA from uninoculated N9436 leaves as the tester. A total of 120 positive clones were randomly chosen from the SSH-cDNA library and were amplified with sp6 and t7 primers to examine the insert size. After screening the repeated and redundant sequences, 59 expressed sequence tags (EST) were acquired. Nucleic acid and protein homology search were performed using the basic local alignment search tool (BLAST) program with the default settings at NCBI website (http://www.ncbi.nlm.nih.gov). BlastX results in nr-protein database revealed that 23 ESTs were highly homologous with known proteins involved in primary metabolism, energy metabolism, transport, signal transduction, and disease resistance and defenses. BlastNr results showed that 47 and 10 ESTs had high identities with known Unigene and function-unknown ESTs, respectively, and two ESTs matched none in the nr-database. Twenty-one ESTs were both in the nucleic acid and protein databases, including seven ESTs associated with powdery mildew resistance. Among them, one was responsible for signal transduction and six for systemic acquired resistance (SAR) system.
Keywords wheat      powdery mildew      suppression subtraction hybridization (SSH)      expressed sequence tags (EST)      
Issue Date: 05 March 2010
URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-010-0094-y     OR     http://academic.hep.com.cn/fag/EN/Y2010/V4/I1/10
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