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Synthesis of artificial antigens of diethylstilbestrol
and preparation of its antibody |
| Qiujia FAN,Xianghong WANG,Qunbiao ZHOU,Li WANG,Yuan ZHAO, |
| College of Food Science
and Technology, Agricultural University of Hebei, Baoding 071001,
China; |
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Abstract Diethylstilbestrol-hemi-succinate (DES-HS) and diethylstilbestrol-monocarboxylic-propyl-ether (DES-MCPE) were synthesized in different ways. The two kinds of haptens were conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to form the immuno-antigens and the coating-antigen, respectively. The antibody with high titer was obtained after immunising rabbits. The results showed that the antibody had a high affinity to diethylstilbestrol tested by enzyme-linked immunosorbent assay (ELISA) and Western blot. This study lays a basis for further research on the immune test kit for the DES remnant.
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| Keywords
diethylstilbestrol
hapten
artificial antigen
antibody
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Issue Date: 05 June 2010
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