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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2010, Vol. 4 Issue (1) : 31-36     DOI: 10.1007/s11703-010-0099-6
Research articles |
Expression analysis of RUS1 and construction of RUS1 plant expressing vector
Qiaoyun WENG1,Jihong XING1,Jingao DONG1,Zhiyong LI2,Zhiping DONG2,
1.Molecular Plant Pathology Lab., College of Life Science, Agricultural University of Hebei, Baoding 071001, China; 2.National Millet Improvement Center, Institute of Millet Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050000, China;
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Abstract  RUS1 was one of the disease resistance gene analogs obtained from Setaria italica Beauv. Semi-quantitative RT-PCR analysis result showed that RUS1 gene could be induced by Uromyces setariae-italicae and had relation to the resistance response of Setaria italica Beauv. against Uromyces setariae-italicae infection. Promoter sequence of RUS1 was obtained by the method of Genome Walking, and its length was 675 bp. RUS1 promoter and pCAMBIA1300 vector were fused to construct RUS1∷GUS vector. GUS histochemical staining result showed that promoter could activate gene expression. RUS1 gene (including the promoter sequence) was obtained through PCR amplification and then fused with pCAMBIA1300 vector to construct pCAMBIA1300∶RUS1 plant expressing vector. The research laid a foundation for gene functional identification of RUS1.
Keywords Setaria italica      RUS1      semi-quantitative RT-PCR      GUS histochemical staining      plant expressing vector      
Issue Date: 05 March 2010
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