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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2010, Vol. 4 Issue (1) : 121-127     DOI: 10.1007/s11703-010-0101-3
Research articles |
Unigene derived SSR analysis for the Fugu rubripes and insights into the characteristics of EST-SSR distribution in tissues/organs
Jiaqing WANG1,Lin HOU2,
1.College of Ocean, Agricultural University of Hebei, Qinhuangdao 066003, China; 2.College of Life Sciences, Liaoning Normal University, Dalian 116029, China;
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Abstract  A total of 11348 Unigene derived ESTs from five cDNA libraries corresponding to five different tissues/organs were screened for EST-SSR, and 1097 EST–SSRs (9.67%) were detected. There were considerable variations in the amount of EST-SSRs among the five tissues/organs. In all the tissues/organs, trinucleotide repeats (67.74%) were found to be most abundant. The trinucleotide repeats GCG were the most abundant in three tissues/organs that ranged from 80.2% in skin, 83.3% in gut, to 90.2% in fin. Dinucleotide was the second abundant repeat type (13.58%), followed by tetra-(12.38%), hexa-(5.34%), and penta-(0.96%) nucleotide repeats. Furthermore, the results of the present study also indicated that the EST-SSR motifs were not randomly distributed in tissues/organs of fugu, such as trinucleotide repeats were found to be strikingly abundant in fin, followed by skin and gut, tetra-nucleotide repeats were especially rich in muscle, while pentanucleotide repeats were less common in any tissue or organ. Most of the EST-SSRs in five tissues/organs that were assigned functions during the present study represent housekeeping genes.
Keywords Unigene      Fugu rubripes      EST-SSRs      tissues/organs      
Issue Date: 05 March 2010
URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-010-0101-3     OR     http://academic.hep.com.cn/fag/EN/Y2010/V4/I1/121
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