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Sequence variations of PDHA1 gene in Triticeae species allow for identifying
wheat-alien introgression lines |
| Juqing JIA,Guangrong LI,Cheng LIU,Jianping ZHOU,Zujun YANG, |
| School of Life Science
and Technology, University of Electronic Science and Technology of
China, Chengdu 610054, China; |
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Abstract In order to develop a molecular marker for the detection of alien chromatin, an allele-specific primer targeting pyruvate dehydrogenase (PDHA1) gene was used to analyze 12 taxa representing different basic genomes in Triticeae. Amplification products with different sizes were generated among species. The sequence alignments indicated that the PDHA1 genes contained some deletions/insertions of Miniature Inverted-repeat Terminal Elements (MITE) and simple sequence repeats (SSRs), thus suggesting that the Triticeae genomes have been rapidly evolving during speciation. The genome-specific amplicons and chromosomal location of PDHA1 gene on Triticeae genomes can be used to trace the corresponding alien chromatins from Aegilops, Secale and Dasypyrum species in wheat background.
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| Keywords
PDHA1 gene
Triticeae species
marker assisted selection
phylogeny
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Issue Date: 05 June 2010
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