|
|
|
In vitro micro
propagation of “sand pear” Pyrus
pyrifolia (Burm. f.) Nakai |
| Zafrul HAQ,Z. A. KALOO, |
| Plant Tissue Culture
Laboratory, Department of Botany, University of Kashmir, Hazratbal
Srinagar-190006, India; |
|
|
|
|
Abstract This study was undertaken to standardize protocols for in vitro explant establishment, shoot multiplication and in vitro rooting for Pyrus pyrifolia, growing in the Kashmir valley. The explant used was nodal cuttings of 2–3cm size with one or two axillary buds. The highest explant establishment (over 90%) was observed during spring while the lowest (60%) explant establishment frequency was observed during winter season. The shoots sprouted from the axillary buds were excised and subcultured on MS basal medium. The highest shoot multiplication rate (130 shoots from a single nodal cutting) was obtained on MS medium supplemented with BAP 2.2 mg·L−1 and kinetin 1 mg·L−1 after four weeks. The shoots were inoculated for rooting in root induction medium of half strength MS salts supplemented with plant growth regulators like IBA, IAA, NAA, and 2, 4-D. However, the best rooting response (8.50 roots per shoot) was obtained in combination of hormones (IBA+ NAA) 0.25 mg·L−1 each. The rooted micro shoots were acclimatized under decreasing humidity regime (95% RH to 75% RH over a period of 5±1 weeks). The highest ex vitro plantlet survival percentage (56.50%) of Pyrus pyrifolia was observed on sand+ soil (1∶1 v/v).
|
| Keywords
Pyrus pyrifolia
micro propagation
shoot multiplication
nodal cuttings
Murashige and Skoog
|
|
Issue Date: 05 September 2010
|
|
|
Cheng T Y (1978). Clonal propagation of woody plantspecies through tissue culture techniques. Proc Int Plant Propag Soc, 28: 139–155
|
|
Dalal A M, Das B, Sharma A K, Mir M A, Sounduri A S (2006). In vitro cloning of apple (Malus domestica Borkh) employing forced shoottip cultures of MQ root stock. Indian J Biotechnol, 5: 543–550
|
|
James D J, Thurbon I J (1979). Rapid in vitro rooting of the apple root stock MQ. J Hortic Sci, 54: 309–311
|
|
Jones O P (1979). Propagation in vitro of apple trees and other woody fruit plants;methods and applications. Sci Hortic (Amsterdam), 30: 44
|
|
Jones O P, Hopgood M E (1979). The successful propagation in vitro of two rootstocks of Prunus: The plum rootstock Pixy (P. insititia) and the cherry rootstock F 12 /1(P. avium). J Hortic Sci, 54: 63–66
|
|
Murashige T, Skoog F (1962). A revised medium for rapid growth and bio-assays with tobacco tissueculture. Physiol Plant, 15: 473–497
doi: 10.1111/j.1399-3054.1962.tb08052.x
|
|
Poudyal B K , Zhang Y, Du G (2008). Adventitious shoot regeneration from the leaves of some pear varities growth in vitro. Front Agric China, 2(1): 82–92
doi: 10.1007/s11703-008-0016-4
|
|
Singha S (1980). In vitro propagation of Seckel pear. In: Proc Conf Nursery production offruit plants through tissue culture- Applications and feasibility. USDA ARRNE-II Beltsville MD, 59–63
|
|
Sriskandarajah S, Mullins M G (1981). Micro propagation of the Granny Smith apple: Factorsaffecting root formation in vitro. J Hortic Sci, 56: 71–76
|
|
Sriskandarajah S, Mullins M G, Nair Y (1982). Induction of adventitious rooting in vitro in difficult to propagate cultivars of apple. Plant Sci Lett, 24: 1–9
doi: 10.1016/0304-4211(82)90002-5
|
|
Tabachnick L, Kester D E (1977). Shoot culture from almond and almond × peach hybridclones in vitro. HortScience, 12: 545–547
|
|
Thakur A, Kanwar J S (2008a). Micro propagation of “Wild Pear” Pyrus pyrifolia (Burm f.) Nakai. I. Explantestablishment and shoot multiplication. Not Bot Hort Agrobot Cluj, 36(1): 103–108
|
|
Thakur A, Kanwar J S (2008b). Micro propagation of “Wild Pear” Pyrus pyrifolia (Burm f.) Nakai. II. Inductionof rooting. Not Bot Hort Agrobot Cluj, 36(2): 104–111
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
