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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2010, Vol. 4 Issue (3) : 308-316     DOI: 10.1007/s11703-010-1020-z
Research articles |
Identification of senescence-related genes by cDNA-AFLP in cotton ( Gossypium hirsutum L.)
Chengjin GUO1,Jinfeng ZHAO1,Cundong LI1,Kai XIAO1,Haina ZHANG2,Chuanfan SUN3,Juntao GU4,
1.College of Agronomy, Agricultural University of Hebei, Baoding 071001, China; 2.College of Agronomy, Agricultural University of Hebei, Baoding 071001, China;Research Institute, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China; 3.Technology Development Center of Rural Areas in China, The Ministry of Science and Technology of the People’s Republic of China, Beijing 100045, China; 4.College of Life Sciences, Agricultural University of Hebei, Baoding 071001, China;
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Abstract  Premature senescence at the late developmental stage occurs frequently in cotton (Gossypium hirsutum L.) production in North China. It is desirable to develop elite cotton cultivars with non-premature senescence and high photosynthetic capacity. In this study, cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis was employed to identify the genes that are related to senescence in cotton. Using 64 primer combinations, about 3000 cDNA fragments were generated, and among them 42 had a markedly up-regulated expression pattern with the leaf growth progression. Based on cloning, sequencing, and Blast search analysis, it was determined that 24 TDFs with putative known biologic functions could be classified into several major categories, such as signal transduction, transcription regulation, stress-responsive, primary and secondary metabolism, nutrients recycling, photosynthesis, cell wall biosynthesis, and senescence-related. TDF31, TDF32 and TDF33, with high similarity to the senescent-regulating genes MAP kinase 9 (MKK9) and non-yellowing protein 1(NYE1) from Arabidopsis and bean senescence-associated receptor-like kinase (SARK) could play possible roles in responding or modulating the leaf senescence in cotton. Therefore, leaf senescence in cotton is a complicated network involving many biological processes. Some putative genes with important modulation functions in regulating or responding to the senescence need to be further analyzed.
Keywords cotton (Gossypium hirsutum L.)      senescence      cDNA-AFLP      gene expression      senescence-related transcript-derived fragments (TDFs)      
Issue Date: 05 September 2010
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