|
|
|
The role of leptin in bovine adipocyte fat metabolism in vitro |
| Cai ZHANG1,Zijun YANG2,Shuling NIU3,Zhe WANG3, |
| 1.College of Livestock
Science & Technology, Henan University of Science and Technology,
Luoyang 471003, China；Department of Animal
Husbandry and Veterinary Medicine, Jilin University, Changchun 130062,
China; 2.College of Livestock
Science & Technology, Henan University of Science and Technology,
Luoyang 471003, China; 3.Department of Animal
Husbandry and Veterinary Medicine, Jilin University, Changchun 130062,
China; |
|
|
|
|
Abstract To identify the effect of leptin on adipocyte, preadipocyte was isolated from male dairy calves and cultured in a monolayer; 5 μg·L−1 bovine leptin were added in culture liquid when the cell differentiated into adipocytes. The result showed that leptin decreased the deposition of triglyceride (TG) and increased the levels of hormone sensitive lipase (HSL) mRNA in cells. At the same time, leptin suppressed the levels of endogenous leptin mRNA, and the levels of leptin receptor (Ob-R) mRNA were raised significantly. It indicated that leptin may induce more fat metabolism directly.
|
| Keywords
leptin
fat metabolism
adipocyte
|
|
Issue Date: 05 September 2010
|
|
|
Casanueva F F, Dieguez C(1999). Neuroendocrine regulation and actions of leptin. Front Neuroendocrinol, 20(4): 317―363
doi: 10.1006/frne.1999.0187
|
|
Fukumoto S, Fujimoto T(2002). Deformation of lipid droplets infixed samples. Histochemistry and Cell Biology, 118(5):423―428
doi: 10.1007/s00418-002-0462-7
|
|
Glasow A, Kiess W, Anderegg U, Berthold A, Bottner A, Kratzsch J(2001). Expression of leptin (Ob) and leptinreceptor (Ob-R) in human fibroblasts: regulation of leptin secretionby insulin. J Clin Endocrinol Metab, 86(9): 4472―4479
doi: 10.1210/jc.86.9.4472
|
|
Livak K J, Schmittgen T D(2001). Analysis of relative gene expression data using real-timequantitative PCR and the 2(-Delta Delta C(T)) Method. Methods, 25(4): 402―408
|
|
Peters J H, Simasko S M, Ritter R C(2007). Leptinanalog antagonizes leptin effects on food intake and body weight butmimics leptin-induced vagal afferent activation. Endocrinology, 148(6): 2878―2885
doi: 10.1210/en.2006-1320
|
|
Philip J, Scarpace, Michael M, Zhang Y, Cheng Kit-Yan, Nihal T(2007). Leptinantagonist reveals an uncoupling between leptin receptor signal transducerand activator of transcription 3 signaling and metabolic responseswith central leptin resistance. J PharmacolExp Ther, 320: 706―712
|
|
Ramirez Z J L, Castro M F, Kuri H W(1992). Quantitation of adiposeconversion and triglycerides by staining in intracyctoplasmic lipidswith oil red O. Histochemistry, 97(6): 493―497
doi: 10.1007/BF00316069
|
|
Ramsay T G(2001). Porcine leptin alters insulin inhibitionof lipolysis in porcine adipocytes in vitro. J Anim Sci, 79(3): 653―657
|
|
Rodbell M(1964). Metabolism of isolated fat cells. J Biol Chem, 239: 375―380
|
|
Spiegelman B M, Flier J S(2001). Obesity and the regulation of energy balance. Cell, 104(4): 531―543
|
|
Van Harmelen V, Rohrig K, Hauner H(2004). Comparisonof proliferation and differentiation capacity of human adipocyte precursorcells from the omental and subcutaneous adipose tissue depot of obesesubjects. Metabolism, Clinical and Experimental, 53(5): 632―637
doi: 10.1016/j.metabol.2003.11.012
|
|
Van R L, Bayliss C E, Roncari D A(1976). Cytologicaland enzymological characterization of adult human adipocyte precursorsin culture. J Clin Invest, 58(3): 699―704
doi: 10.1172/JCI108516
|
|
Xia C, Wang Z, Niu S L, Zhang C, Zhang H Y(2004). Cultureof calf preadipocyte and establishment of its proliferation and differentiationmodel. Chinese Journal of Veterinary Scienceand Technology, 34(5): 26―29 (in Chinese)
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
