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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2010, Vol. 4 Issue (4) : 430-437    https://doi.org/10.1007/s11703-010-1036-4
RESEARCH ARTICLE
Kinetics of microbial immobilization of phosphorus in a weathered subtropical soil following treatment with organic amendments and Pseudomonas sp.
Rong SHENG1,2, Min HUANG1,3, Heai XIAO1(), Tida GE1, Jinshui WU1, Chengli TONG1, Zhoujin TAN4, Daping XIE2
1. Key Laboratory for Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; 2. College of Bio-Safety Science and Technology, Hunan Agricultural University, Changsha 410128, China; 3. School of Resources and Environmental Engineering, Wuhan University of Technology, Wuhan 430081, China; 4. School of Preclinical Medicine, TCM University of Hunan, Changsha 410208, China
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Abstract

To understand the role of microbial processes in phosphorus (P) immobilization in a weathered subtropical soil, the effects of application of a phosphate-solubilization microorganism strain (Pseudomonas sp. 2VCP1) on P availability in soil, dynamics in microbial biomass P (Bp), microbial biomass C (Bc) and Olsen-P were investigated during a 60-d laboratory incubation. The included treatments were P. sp. inoculums at×106 cfu·g-1 soil (CKM); glucose at 5 g·kg-1 soil (G); G with P. sp. inoculum (GM); rice straw at 5 or 10 g·kg-1 soil (5S or 10S); 5S and 10S with P. sp. inoculum (5SM and 10SM). The results indicated that the amount of soil Bc increased about 3.2, 1.7, and 2.6 times for G, 5S and 10S compared to the control (no organic amendment and P. sp.; CK), respectively. The amount of soil Bp for G and 10S almost doubled during the first 7 d, then remained relatively steady. The amount of Olsen-P in G, 5S and 10S showed a significant decrease (0–5.4 mg P·kg-1 soil) during the 60-d incubation compared to CK. However, changes in soil Bp between the treatments inoculated with P. sp. (CKM, G, 5SM, 10SM) and the uninoculated controls (CK, G, 5S, 10S) were not significant during the 60-d incubation period, whereas a small increase in Bp of the GM treatment was seen up to day 11. The amount of soil Bc in CKM, GM, 5SM and 10SM had increased but not greater than 20% compared to their corresponding uninoculated control. The amount of Olsen-P increased but not greater than 0.88 mg P·kg-1 soil. The result illustrated that there were a few effects on soil P immobilization following inoculation with P. sp. in the soil, whereas organic amendments can significantly motivate the soil native microorganisms to immobilize phosphorus.

Keywords soil microbial biomass      phosphorus-solubilization microorganism      organic substance     
Corresponding Author(s): XIAO Heai,Email:haxiao@isa.ac.cn   
Issue Date: 05 December 2010
 Cite this article:   
Rong SHENG,Min HUANG,Heai XIAO, et al. Kinetics of microbial immobilization of phosphorus in a weathered subtropical soil following treatment with organic amendments and Pseudomonas sp.[J]. Front Agric Chin, 2010, 4(4): 430-437.
 URL:  
https://academic.hep.com.cn/fag/EN/10.1007/s11703-010-1036-4
https://academic.hep.com.cn/fag/EN/Y2010/V4/I4/430
treatmenttype and quantitya of amendment
rice straw/(g·kg-1 soil)glucose/(g·kg-1 soil)Pseudomonas sp./(×106 cfu·g-1 soil)
CK000
CKM001
G050
GM051
5S500
5SM501
10S1000
10SM1001
Tab.1  Experiment design in this study
Fig.1  Changes in soil microbial biomass C in the subtropical soil between the treatments with sp. 2VCP1 (CKM, GM, 5SM, 10SM) and corresponding controls (CK, G, 5S, 10S) during 60-d incubation period.
Note: Bars indicate the standard deviation of the mean, the same below. *, ** indicated significant difference between treatments amended with and without sp. 2VCP1 at 0.05 and 0.01 level, respectively, the same below.
Fig.2  Changes in soil microbial biomass P in the subtropical soil between the treatments with sp. 2VCP1 (CKM, GM, 5SM, 10SM) and corresponding controls (CK, G, 5S, 10S) during 60-d incubation period.
Fig.3  Changes in Olsen-P in the subtropical soil between the treatments with sp. 2VCP1 (CKM, GM, 5SM, 10SM) and corresponding controls (CK, G, 5S, 10S) during 60-d incubation period.
treatmentincubation days
37111421314560
CK23.7±1.120.5±0.428.4±1.629.3±1.826.8±2.038.9±3.239.1±0.534.8±0.2
CKM28.0±1.823.2±1.935.5±2.5**40.1±3.3**30.7±2.535.3±2.633.8±0.935.0±1.3
G43.3±2.527.6±1.5**28.8±1.7**24.2±1.619.5±1.724.4±2.023.6±1.525.8±1.9
GM40.5±3.319.1±1.221.5±2.119.1±2.616.7±2.924.1±1.925.9±0.925.0±2.2
5S37.9±3.931.8±0.534.4±3.637.3±1.2**27.8±4.534.1±1.932.1±1.330.5±2.0
5SM45.1±2.430.1±3.831.0±3.329.1±3.924.4±3.434.3±3.735.4±0.534.8±1.6
10S58.7±2.023.6±2.127.6±2.625.2±3.821.2±2.227.0±3.026.2±3.026.1±2.8
10SM62.5±3.320.6±2.422.4±3.320.7±2.318.3±0.823.7±3.328.9±0.523.7±4.0
Tab.2  Dynamics of C-to-P ratio of microbial biomass during the 60-d incubation period in soil under different treatments (mean±SD)
1 Agbenin O J, Adeniyi T (2005). The microbial biomass properties of a savanna soil under improved grass and legume pastures in northern Nigeria. Agriculture, Ecosystems and Environment , 109(3-4): 245–254
doi: 10.1016/j.agee.2005.03.003
2 Bolan N S (1991). A critical review on the role of mycorrhizal fungi in the uptake of phosphorus by plant. Plant and Soil , 134(2): 189–207
doi: 10.1007/BF00012037
3 Bremner J M (1965). Total nitrogen. In: Black C A, ed. Methods of Soil Analysis, vol 2 . Madison: American Society of Agronomy, 1149–1178
4 Brookes P C, Powlson D S, Jenkinson D S (1982). Measurement of microbial biomass phosphorus in soil. Soil Biology and Biochemistry , 14(4): 319–329
doi: 10.1016/0038-0717(82)90001-3
5 Brookes P C, Powlson D S, Jenkinson D S (1984). Phosphorus in the soil microbial biomass. Soil Biology and Biochemistry , 16(2): 169–175
doi: 10.1016/0038-0717(84)90108-1
6 Buehler S, Oberson A, Rao I M, Friesen D K, Frossard E (2002). Sequential phosphorus extraction of a 32P-labelled Oxisol under contrasting agricultural systems. Soil Science Society of America , 66: 868–877
doi: 10.2136/sssaj2002.0868
7 Bünemann E K, Bossio D A, Smithson P C, Frossard E, Oberson A (2004). Microbial community composition and substrate use in a highly weathered soil as affected by crop rotation and P fertilization. Soil Biology and Biochemistry , 36(6): 889–901
doi: 10.1016/j.soilbio.2004.02.002
8 Chuang C C, Kuo Y L, Chao C C, Chao W L (2007). Solubilization of inorganic phosphates and plant growth promotion by Aspergillus niger. Biology and Fertility of Soils , 43(5): 575–584
doi: 10.1007/s00374-006-0140-3
9 Cunningham J E, Kuiack C (1992). Production of citric and oxalic acids and solubilization of calcium phosphate by Penicillium bilaii. Applied and Environmental Microbiology , 58(5): 1451–1458
10 Friesen D K, Rao I M, Thomas R J, Oberson A, Sanz J I (1997). Phosphorus acquisition and cycling in crop and pasture systems in low fertility tropical soils. Plant and Soil , 196(2): 289–294
doi: 10.1023/A:1004226708485
11 George T S, Turner B L, Gregory P J, Cade-Menun B J, Richardson A E (2006). Depletion of organic phosphorus from Oxisols in relation to phosphatase activities in the rhizosphere. European Journal of Soil Science , 57(1): 47–57
doi: 10.1111/j.1365-2389.2006.00767.x
12 Gijsman A J, Oberon A, Friesen D K, Sanz J I, Thomas R J (1997). Nutrient cycling through microbial biomass under rice-pasture rotation replacing native savanna. Soil Biology and Biochemistry , 29(9-10): 1433–1441
doi: 10.1016/S0038-0717(97)00045-X
13 Gyaneshwar P, Kumar G N, Parekh L J (1998). Effect of buffering on the phosphate solubilizing ability of microorganisms. World Journal of Microbiology and Biotechnology , 14(5): 669–673
doi: 10.1023/A:1008852718733
14 Kalembasa S J, Jenkinson D S (1973). A comparative study of titrimetric and gravimetric methods for the determination of organic carbon in soil. Journal of the Science of Food and Agriculture , 24(9): 1085–1090
doi: 10.1002/jsfa.2740240910
15 Kouno K, Wu J, Brookes P C (2002). Turnover of biomass C and P in soil following incorporation of glucose or ryegrass. Soil Biology and Biochemistry , 34(5): 617–622
doi: 10.1016/S0038-0717(01)00218-8
16 Kucey R M N (1983). Phosphate solubilizing bacteria and fungi in various cultivated and virgin Alberta soils. Canadian Journal of Soil Science , 63(4): 671–678
doi: 10.4141/cjss83-068
17 Kwabiah A B, Palm C A, Stoskopt N C, Voroney R P (2003). Response of soil microbial biomass dynamics to quantity of plant materials with emphasis on P availability. Soil Biology and Biochemistry , 35(2): 207–216
doi: 10.1016/S0038-0717(02)00253-5
18 Li S T, Zhou J M, Wang H Y, Chen X Q, Du C W (2003). Characteristics of fixation and release of phosphorus in three soils. Acta Pedologica Sinica , 40: 908–914 (in Chinese)
19 Murphy J, Riley J P (1962). A modified single solution method for the determination of phosphate in natural waters. Analytica Chimica Acta , 27: 31–36
doi: 10.1016/S0003-2670(00)88444-5
20 Oberson A, Friesen D K, Rao I M, Bühler S, Frossard E (2001). Phosphorus transformations in an Oxisol under contrasting land-use systems: The role of the soil microbial biomass. Plant and Soil , 237(2): 197–210
doi: 10.1023/A:1013301716913
21 Oberson A, Friesen D K, Tiessen H, Morel C, Stahel W (1999). Phosphorus status and cycling in native savanna and improved pastures on an acid low-P Colombian Oxisol. Nutrient Cycling in Agroecosystems , 55(1): 77–88
doi: 10.1023/A:1009813008445
22 Oehl F, Oberson A, Probst M, Fliessbach A, Roth H R, Frossard E (2001). Kinetics of microbial phosphorus uptake in cultivated soils. Biology and Fertility of Soils , 34(1): 31–41
doi: 10.1007/s003740100362
23 Olsen S R, Somers L E (1982). Phosphorus. In: Page A L, Miller R H, Keene D R, eds. Methods of Soil Analysis, vol 2 . Madison: Soil Science Society of America, 403–448
24 Perrott K W, Sarathchandra S U, Waller J E (1990). Seasonal storage and release of phosphorus and potassium by organic matter and the microbial biomass in a high-producing pastoral soil. Australian Journal of Soil Research , 28(4): 593–608
doi: 10.1071/SR9900593
25 Shi X Z, Yu D S, Warner E D, Sun W X, Petersen G W, Gong Z T, Lin H (2005). Cross–reference system for translating between genetic soil classification of China and soil taxonomy. Soil Science Society of America , 70(1): 78–83
doi: 10.2136/sssaj2004.0318
26 Vance E D, Brookes P C, Jenkinson D S (1987). An extraction method for measuring soil microbial biomass C. Soil Biology and Biochemistry , 19(6): 703–707
doi: 10.1016/0038-0717(87)90052-6
27 Vassilev N, Vassileva M, Fenice M, Federici F (2001). Immobilized cell technology applied in solubilization of insoluble inorganic (rock) phosphates and P plant acquisition. Bioresource Technology , 79(3): 263–271
doi: 10.1016/S0960-8524(01)00017-7
28 Wu J, Brookes P C, Jenkinson D S (1993). Formation and destruction of microbial biomass during the decomposition of glucose and ryegrass in soil. Soil Biology and Biochemistry , 25(10): 1435–1441
doi: 10.1016/0038-0717(93)90058-J
29 Wu J, He Z L, Wei W X, O’Donnell A G, Syers J K (2000). Quantifying microbial biomass phosphorus in acid soils. Biology and Fertility of Soils , 32(6): 500–507
doi: 10.1007/s003740000284
30 Wu J, Huang M, Xiao H A, Su Y R, Tong C L, Huang D Y, Syers J K (2007). Dynamics in microbial immobilization and transformations of phosphorus in highly weathered subtropical soil following organic amendments. Plant and Soil , 290(1-2): 333–342
doi: 10.1007/s11104-006-9165-5
31 Wu J, Joergensen R G, Pommerening B, Chaussod R, Brookes P C (1990). Measurement of soil microbial biomass C by fumigation-extraction- an automated procedure. Soil Biology and Biochemistry , 22(8): 1167–1169
doi: 10.1016/0038-0717(90)90046-3
32 Zaidi A, Khan M S (2005). Interactive effect of rhizospheric microorganisms on growth, yield and nutrient uptake of wheat. Journal of Plant Nutrient , 28(12): 2079–2092
doi: 10.1080/01904160500320897
33 Zaidi A, Khan M S, Aamil M (2004). Bio-associative effect of rhizospheric microorganisms on growth, yield and nutrient uptake of greengram. Journal of Plant Nutrient , 27(4): 601–612
doi: 10.1081/PLN-120030370
34 Zaidi A, Khan M S, Amil M (2003). Interactive effect of rhizospheric microorganisms on yield and nutrient uptake of chickpea (Cicer arietinum L.). Journal of Plant Nutrient , 19: 15–21
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