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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2010, Vol. 4 Issue (4) : 468-474     DOI: 10.1007/s11703-010-1045-3
RESEARCH ARTICLE |
Effects of drought on electrical impedance spectroscopy parameters in stems of Pinus bungeana Zucc. seedlings
Aifang WANG1, Gang ZHANG2()
1. College of Forestry, Agricultural University of Hebei, Baoding 071001, China; 2. College of Horticulture, Agricultural University of Hebei, Baoding 071001, China
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Abstract  

The effects of drought during preplanting (three treatments: soil relative water content (RWC) 75%–80%, 55%–60%, 35%–40%; B1, 2, and 3, respectively) and postplanting (four treatments: RWC 75%–80%, 55%–60%, 35%–40%, 15%–20%; A1, 2, 3, and 4, respectively) on electrical impedance spectroscopy (EIS) parameters in the stems of Pinus bungeana Zucc. seedlings were investigated by using 4-year-old container seedlings. Stem impedance spectra were modeled by a distributed circuit element model (2-DCE), which showed the extracellular and intracellular resistance (re and ri), relaxation time (τ1 and τ2), and distribution coefficient (ψ1 and ψ2) of relaxation time. After preplanting B3 drought treatment, re and ri increased significantly with the increase of soluble sugar of the stem, measured by enthronlsulphuric acid method. After four weeks postplanting A4 drought treatment, relative conductivity, and soluble sugar of stem increased significantly, and re of stem decreased significantly and continually, indicating that the cell membrane of stem cells was disrupted by severe drought. After five weeks drought treatment, τ1 of stem under A4 treatment decreased significantly, and ψ2 of stem under A2, A3, and A4 treatments was higher than that of A1 treatment. Briefly, drought made re, ri, τ1, and ψ2 of stem change regularly, but re was found to be the most informative and useful parameter measured if used as a single index to assess the drought resistance of P. bungeana Zucc. seedlings.

Keywords drought      Pinus bungeana Zucc.      stem      extracellular resistance      intracellular resistance      relaxation time     
Corresponding Authors: ZHANG Gang,Email:zhanggang1210@126.com   
Issue Date: 05 December 2010
URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-010-1045-3     OR     http://academic.hep.com.cn/fag/EN/Y2010/V4/I4/468
Fig.1  Effects of preplanting drought treatments on electrical impedance spectroscopy (EIS) parameters of stems in Zucc. seedlings
Note: B1: 75%-80% soil RWC, B2: 55%-60%, and B3: 35%-40%.
Fig.2  Effects of preplanting drought treatments on water content of stems in Zucc. seedlings
Note: B1: 75%-80%, B2: 55%-60%, and B3: 35%-40%.
Fig.3  Effects of preplanting drought treatments on soluble sugar concentration of stems in Zucc. seedlings
Note: B1: 75%-80%, B2: 55%-60%, and B3: 35%-40%.
Fig.4  Effects of preplanting drought treatments on relative conductivity of stems in Zucc. seedlings
Note: B1: 75%-80%, B2: 55%-60%, and B3: 35%-40%.
Fig.5  Effects of postplanting drought treatments on extracellular resistance of stems in Zucc. seedlings with the phase of drought treatments
Note: A1-A4 are postplanting drought treatments, A1: 75%-80% soil RWC, A2: 55%-60%, A3: 35%-40%, and A4: 15%-20%.
Fig.6  Effects of postplanting drought treatments on intracellular resistance of stems in Zucc. seedlings with the phase of drought treatments
Note: A1-A4 are postplanting drought treatments, A1: 75%-80%, A2: 55%-60%, A3: 35%-40%, and A4: 15%-20%.
Fig.7  Effects of postplanting drought treatments on relaxation time ( and ) of stems in Zucc. seedlings with the phase of drought treatments
Note: A1-A4 are postplanting drought treatments, A1: 75%-80%, A2: 55%-60%, A3: 35%-40%, and A4: 15%-20%.
Fig.8  Effects of postplanting drought treatments on the distribution coefficient ( and ) of relaxation time ( and ) of stems in Zucc. seedlings with the phase of drought treatments
Note: A1-A4 are postplanting drought treatments, A1: 75%-80%, A2: 55%-60%, A3: 35%-40%, and A4: 15%-20%.
Fig.9  Effects of postplanting drought treatments on the water content of stems in Zucc. seedlings with the phase of drought treatments
Note: A1-A4 are postplanting drought treatments, A1: 75%-80%, A2: 55%-60%, A3: 35%-40%, and A4: 15%-20%.
Fig.10  Effects of postplanting drought treatments on soluble sugar concentration of stems in Zucc. seedlings with the phase of drought treatments
Note: A1-A4 are postplanting drought treatments, A1: 75%-80%, A2: 55%-60%, A3: 35%-40%, and A4: 15%-20%.
Fig.11  Effects of postplanting drought treatments on the relative conductivity of stems in Zucc. seedlings with the phase of drought treatments
Note: A1-A4 are postplanting drought treatments, A1: 75%-80%, A2: 55%-60%, A3: 35%-40%, and A4: 15%-20%.
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