Effects of traditional Chinese medicine “Yimu Shenghuatang” on cytochrome P450 in cow inflammatory endometrial cells

doi:10.1007/s11703-010-1046-6

Front Agric Chin

2011, Vol. 5

Issue (1) : 102-105 https://doi.org/10.1007/s11703-010-1046-6

RESEARCH ARTICLE

Effects of traditional Chinese medicine “Yimu Shenghuatang” on cytochrome P450 in cow inflammatory endometrial cells

Jinliang DU¹, Jianhua QIN¹, Jingsheng CHU², Lina XU¹, Yuzhong MA¹(

)

1. College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China; 2. College of Traditional Chinese Medicine, Agricultural University of Hebei, Dingzhou 073000, China

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Abstract

To investigate the metabolism mechanism of traditional Chinese medicine “Yimu Shenghuatang” on dairy cow endometrial cells, the primary cultured endometrial cells in dairy cows were isolated by a collagenase digestion method, and an inflammatory model of the endometrial cells by lipopolysaccharide (LPS) was made. The cells were treated with gradient concentrations of LPS, the cultured supernatants were collected in different periods, and the contents of TNF-α, IL-1β were determined by Enzyme-linked immunosorbent assay (ELISA). The results showed that 100 ng·mL^-1 LPS was the optimal concentration to induce the inflammation of cultured endometrial cells. The inflammatory endometrial cells were treated with traditional Chinese medicine “Yimu Shenghuatang” and its main components during the period of 24–72 h, and the expression of cytochrome P450 in cow endometrial cells was determined by Western blot. Our result lays a foundation for treating cow endometritis by using traditional Chinese medicines.

Keywords dairy cow inflammatory endometrial cell CYP450 traditional Chinese medicine “Yimu Shenghuatang”

Corresponding Author(s): MA Yuzhong,Email:dkma@hebau.edu.cn

Issue Date: 05 March 2011

Cite this article:

Jinliang DU,Jianhua QIN,Jingsheng CHU, et al. Effects of traditional Chinese medicine “Yimu Shenghuatang” on cytochrome P450 in cow inflammatory endometrial cells[J]. Front Agric Chin, 2011, 5(1): 102-105.

URL:

https://academic.hep.com.cn/fag/EN/10.1007/s11703-010-1046-6
https://academic.hep.com.cn/fag/EN/Y2011/V5/I1/102

Tab.1 Inhibitory action of LPS on cow endometrial cell growth ( = 6)

Tab.2 Effects of LPS on the contents of TNF-α and IL-1β in endometrial cells ( = 6)

Fig.1 CYP450 expression in dairy cow endometrial cells induced by “Yimu Shenghuatang”

Fig.2 CYP450 expression in dairy cow endometrial cells induced by

Fig.3 CYP450 expression in dairy cow endometrial cells induced by

Fig.4 CYP450 expression in dairy cow endometrial cells induced by

Fig.5 CYP450 expression in dairy cow endometrial cells induced by

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