Knockdown of <italic>ACS9</italic> expression in <italic>Arabidopsis</italic> decreases the tolerance to salt and osmotic stress

doi:10.1007/s11703-011-1022-5

Front Agric Chin

2011, Vol. 5

Issue (2) : 181-186 https://doi.org/10.1007/s11703-011-1022-5

RESEARCH ARTICLE

Knockdown of ACS9 expression in Arabidopsis decreases the tolerance to salt and osmotic stress

Xueli HAN, Yonggang PAN, Yingchao LIU(

), Jihong XING, Jingao DONG(

)

Molecular Plant Pathology Lab, College of Life Science, Agricultural University of Hebei, Baoding 071001, China

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Abstract

Based on the DNA sequence of ACS9, two produced fragments were subcloned into binary vector pCAMBIA1300 in antisense and sense orientations, and the generated RNA interference (RNAi) vector was then transformed into Arabidopsis thaliana. The stress resistance function of ACS9 gene in Arabidopsis thaliana was researched by determination of stress resistance physiologic indexes, NaCl and PEG6000 resistance. The results showed that the inhibition of ACS9 expression enhanced the sensitivity to high concentration NaCl (150 mmol/L) and PEG6000(7%) in Arabidopsis thaliana seeding stage. The proline contents and water loss rates in transgenic plants were 0.68 and 1.4 times higher than those in the wild-type leaves, respectively, indicating that the inhibition of ACS9 expression due to salt and drought resistant was reduced and suggested that ACS9 gene played important roles in plant salt and drought tolerance.

Keywords ACS9 gene RNAi vector functional analysis stress resistance

Corresponding Author(s): LIU Yingchao,Email:liuyingchao@hebau.edu.cn; DONG Jingao,Email:dongjingao@126.com

Issue Date: 05 June 2011

Cite this article:

Xueli HAN,Yonggang PAN,Yingchao LIU, et al. Knockdown of ACS9 expression in Arabidopsis decreases the tolerance to salt and osmotic stress[J]. Front Agric Chin, 2011, 5(2): 181-186.

URL:

https://academic.hep.com.cn/fag/EN/10.1007/s11703-011-1022-5
https://academic.hep.com.cn/fag/EN/Y2011/V5/I2/181

Fig.1 Restriction digestion analysis of pCAMBIA1300-i. Note: M is DL2000 DNA marker; 1 is I and I; 2 is I and HI, and 3 is HI and I.

Fig.2 PCR identification of transgenic plants. Note: M is DL2000 marker; l-4 and 6 are transgenic plants; 5 are nontransgenic plants, and 7 is pCAMBIA1300 plasmid (positive control); 8 is negative control, and 9 is water control.

Fig.3 Expression detection of in transgenic plants. Note: l is wild-type; 2-8 are transgenic plants.

Fig.4 Seeding growth of transgenic plants and wild-type plants when etiolated for 10 days. Note: A shows the results for hypocotyl and root length, and B shows the results for growth of hypocotyl and root. WT is wild-type plants, and M is mature transgenic plants.

Fig.5 Germination and growth of transgenic plants and wild type plants when seeds were treated with NaCl for 10 days. Note: A shows the seed germination of transgenic plants and wild type plants; B shows the seed growth of transgenic plants and wild type plants.

Fig.6 Root length transgenic plants and wild-type plants seeding with PEG6000.

Fig.7 Proline accumulation of transgenic plants and wild-type plants with 150 mmol/L NaCl for 48 h.

Fig.8 Leaf water loss rate transgenic plants and wild type.

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