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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2011, Vol. 5 Issue (1) : 35-39     DOI: 10.1007/s11703-011-1050-1
RESEARCH ARTICLE |
Induced mutations in chickpea-morphological mutants
Samiullah KHAN(), Kouser PARVEEN, Sonu GOYAL
Mutation Breeding Laboratory, Department of Botany, Aligarh Muslim University, Aligarh-202002(UP), India
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Abstract  

Seeds of two varieties (PUSA-212 and BG-256) of chickpea (BoldItalic L.) were treated with 0.03% concentration of hydrazine hydrate (HZ), methylmethane sulphonate (MMS) and sodium azide (SA) for 6 h. The M2 generation was grown from single plant M1 progeny seeds. Five morphological mutants (dwarf, bushy, one sided branch, narrow leaf and gigas) were isolated in M2 generation. Some of these mutants may be directly used in selection whereas some are useful in combination breeding programs.

Keywords Cicer arietinum L.      chemical mutagens      morphological mutants     
Corresponding Authors: KHAN Samiullah,Email:khan_drsami@yahoo.co.in   
Issue Date: 05 March 2011
URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-011-1050-1     OR     http://academic.hep.com.cn/fag/EN/Y2011/V5/I1/35
mutagen typeNo. of M2 plantNo. of mutants scoredtotal No. of mutantfrequency/%
Pusa-212BG-256
HZ5201512275.19
MMS5131109203.89
SA5010403071.39
Tab.1  Frequency of morphological mutants in various mutagens in M generation
mutant typevarietyNo. of mutants/totalNo. of M2 plantfrequency/%
dwarf mutantPusa-212BG-2566/7505/7840.800.64
bushy mutantPusa-212BG-2564/750*3/784*0.530.38
one sided branch mutantPusa-212BG-2564/7500/7840.53
narrow leaf mutantPusa-212BG-25610/7508/7841.331.02
gigas mutantPusa-212BG-2566/7508/7840.801.02
totalPusa-212BG-25630/75024/7844.003.06
Tab.2  Frequency and spectrum of morphological mutants observed in chickpea varieties Pusa-212 and BG-256 in M generation
Fig.1  Plate-1
Note: 1-5 represent control plant, dwarf mutant, bushy mutant, one side branch mutant, narrow leaf mutant, respectively.
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[1] Mohd Rafiq WANI, Samiullah KHAN, Mohammad Imran KOZGAR. Induced chlorophyll mutations. I. Mutagenic effectiveness and efficiency of EMS, HZ and SA in mungbean[J]. Front Agric Chin, 2011, 5(4): 514-518.
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