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The physical interaction between LdPLCs and Arabidopsis G beta in a yeast two-hybrid system |
Jinglei SUN1, Xiuhua LIU2, Yanyun PAN1() |
1. College of Life Science, Agricultural University of Hebei, Baoding 071001, China; 2. College of Basic Medical, Hebei University, Baoding 071002, China |
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Abstract Phosphoinositide-specific phospholipase C plays pivotal roles in a host of physiologic processes in both animals and plants. Animal PI-PLC is regulated by heterotrimeric G-protein. Plant PI-PLCs are structurally close to the mammalian PI-PLC-ζ isoform, and it is not testified what regulated this isoform enzyme. In this paper, two isoform genes of LdPLC (Pan, 2005) and three subunits of heterotrimeric G-protein in BoldItalic were amplified and recombinated with plasmids of a yeast two-hybrid system. Using this system, we provided the evidence that LdPLC1 and Gβ subunit could be able to interact with each other. This result indicated that LdPLC1 might be regulated by G-protein.
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Keywords
phosphoinositide-specific phospholipase C (PI-PLC)
heterotrimeric G-protein
yeast two-hybrid
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Corresponding Author(s):
PAN Yanyun,Email:pyycell@163.com
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Issue Date: 05 March 2011
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