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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2011, Vol. 5 Issue (2) : 201-208    https://doi.org/10.1007/s11703-011-1064-8
RESEARCH ARTICLE
Physiological and biochemical changes associated with flower development and senescence in Consolida ajacis Nieuwl cv. Violet blue
Shahri WASEEM(), Tahir INAYATULLAH
Plant Physiology and Biochemistry Research Laboratory, Department of Botany, University of Kashmir, Srinagar, India-190006
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Abstract

Flower development of Consolida ajacis cv. Violet blue growing in the Kashmir University Botanic Garden (KUBG) was divided into six stages (I–VI) from the tight bud stage to the senescent stage. The average life span of an individual flower after it is fully open is about 4 days. Membrane permeability of sepal tissues estimated as electrical conductivity of leachates increased during senescence. The content of sugars and soluble proteins in the sepal tissues increased during flower opening and declined thereafter during senescence. The α-amino acid content registered an increase as the flowers opened and senesced. The protease activity increased as the flower progressed toward senescence. Flower opening was closely correlated to the sugar status of sepals, while the increase in the protease activity was commensurate with the decrease in the tissue content of soluble protein levels. The results suggested that the reduction in sugar status and the elevation in protease activity are among the important changes associated with the sepal senescence of Consolida ajacis flowers. SDS-PAGE of protein extracts from sepal tissues suggested a general decrease in the expression of some high molecular weight proteins and an increase in low molecular weight proteins during the flower development and senescence. At this stage, it is not known whether these polypeptides play an important role in the senescence of C. ajacis flowers. Understanding the nature of these proteins can provide new insights into the pathways to execute the senescence and the post-transcriptional regulation of senescence in this flower system.

Keywords α-amino acids      flower senescence      Consolida ajacis      membrane permeability      protease activity      proteins     
Corresponding Author(s): WASEEM Shahri,Email:waseem.bot@gmail.com, waseem_bot@yahoo.com   
Issue Date: 05 June 2011
 Cite this article:   
Shahri WASEEM,Tahir INAYATULLAH. Physiological and biochemical changes associated with flower development and senescence in Consolida ajacis Nieuwl cv. Violet blue[J]. Front Agric Chin, 2011, 5(2): 201-208.
 URL:  
https://academic.hep.com.cn/fag/EN/10.1007/s11703-011-1064-8
https://academic.hep.com.cn/fag/EN/Y2011/V5/I2/201
Fig.1  Flowers of cv. Violet blue in full bloom
Fig.2  Stages of flower development and senescence in cv. Violet blue
Fig.3  Changes in flower diameter (cm) during various stages of flower development and senescence
Fig.4  Changes in fresh mass, dry mass and water content of flowers (mg·flower) during various stages of flower development and senescence
Fig.5  Changes in the electrical conductivity of ion leachates (μS) during various stages of flower development and senescence
Fig.6  Changes in the content of sugar fractions (expressed as mg·g) during various stages of flower development and senescence
Fig.7  Changes in the content of soluble proteins (expressed as mg·g), specific protease activity (expressed as μg tyrosine equivalents liberated per mg protein) and - amino acids (expressed as mg·g) during various stages of flower development and senescence
Fig.8  Changes in the content of total phenols (expressed as mg·g) during various stages of flower development and senescence
Fig.9  Relationship between electrical conductivity of ion leachates and total phenol content of sepal tissues (a), soluble protein content and specific protease activity (b), flower opening and reducing sugar content (c) during various stages of flower development and senescence
Fig.10  12% SDS-PAGE of equal amounts of extractable protein at various stages (I to VI) of flower development and senescence from sepal tissues of
Note: The gel was stained with Coomassie blue. Numbers above the lanes correspond to developmental stages. Molecular weight standards are indicated on the left (kDa) and ca molecular weights of major polypeptides to the right of the gel (kDa).
1 Arora A, Singh V P (2004). Cysteine protease gene expression and proteolytic activity during floral development and senescence in ethylene-insensitive Gladiolus grandiflora. J Plant Biochem Biotechnol , 13: 123–126
2 Ausubel F M, Brent R, Kingston R E, Moore D D, Seidman J C, Struhl K (1989). Current Protocols in Molecular Biology.New York: John Wiley and Sons
3 Azad A K, Ishikawa T Y, Ishikawa Th, Sawa Y, Shibata H (2008). Intracellular energy depletion triggers programmed cell death during petal senescence in tulip. J Exp Bot , 59(8): 2085–2095
doi: 10.1093/jxb/ern066 pmid:18515833
4 Bai S, Willard B, Chapin L J, Kinter M T, Francis D M, Stead A D, Jones M L (2010). Proteomic analysis of pollination-induced corolla senescence in Petunia. J Exp Bot , 61(4): 1089–1109
doi: 10.1093/jxb/erp373 pmid:20110265
5 Beers E P, Woffenden B J, Zhao C (2000). Plant proteolytic enzymes: possible roles during programmed cell death. Plant Mol Biol , 44(3): 399–415
doi: 10.1023/A:1026556928624 pmid:11199397
6 Bieleski R L (1993). Fructan hydrolysis drives petal expansion in the ephemeral daylily flower. Plant Physiol , 103(1): 213–219
pmid:12231928
7 Borochov A, Cho M H, Boss W F (1994). Plasma membrane lipid metabolism of Petunia petals during senescence. Physiol Plant , 90(2): 279–284
doi: 10.1111/j.1399-3054.1994.tb00388.x
8 Celikel F G, van Doorn W G (1995). Solute leakage, lipid peroxidation, and protein degradation during the senescence of Iris tepals. Physiol Plant , 94(3): 515–521
doi: 10.1111/j.1399-3054.1995.tb00962.x
9 Courtney S E, Rider C C, Stead A D (1994). Changes in protein ubiquitination and the expression of ubiquitin-encoding transcripts in daylily petals during floral development and senescence. Physiol Plant , 91(2): 196–204
doi: 10.1111/j.1399-3054.1994.tb00419.x
10 Eason J R, de Vré L A, Somerfield S D, Heyes J A (1997). Physiological changes associated with Sandersonia aurantiaca flower senescence in response to sugar. Postharvest Biol Technol , 12(1): 43–50
doi: 10.1016/S0925-5214(97)00040-9
11 Eason J R, Ryan D J, Pinkney T T, O'Donoghue E M (2002). Programmed cell death during flower senescence: Isolation and characterization of cysteine proteinases from Sandersonia aurantiaca. Funct Plant Biol , 29(9): 1055–1064
doi: 10.1071/PP01174
12 Evans R Y, Reid M S (1988). Changes in carbohydrates and osmotic potential during rhythmic expansion of rose petals. J Am Soc Hortic Sci , 113(6): 884–888
13 Finger F L, Carneiro T F, Barbosa J G (2004). Postharvest senescence of inflorescencias of esporhina (Consolida ajacis). Brasilia , 39: 533–537
14 Guerrero C, de la Calle M, Reid M S, Valpuesta V (1998). Analysis of the expression of two thiol protease genes from daylily (Hemerocallis spp.) during flower senescence. Plant Mol Biol , 36(4): 565–571
doi: 10.1023/A:1005952005739 pmid:9484451
15 Have A T, Woltering E J (1997). Ethylene biosynthetic genes are differentially expressed during carnation (Dianthus caryophyllus L.) flower senescence. Plant Mol Biol , 34: 89–97
16 Hoeberichts F A, de Jong A J, Woltering E J (2005). Apoptotic like cell death marks the early stages of gypsophila (Gypsophila paniculata) petal senescence. Postharvest Biol Technol , 35: 229–236
doi: 10.1016/j.postharvbio.2004.10.005
17 Hopkins M, Taylor C, Liu Z, Ma F, McNamara L, Wang T W, Thompson J E (2007). Regulation and execution of molecular disassembly and catabolism during senescence. New Phytol , 175(2): 201–214
doi: 10.1111/j.1469-8137.2007.02118.x pmid:17587370
18 Ichimura K, Yamada T, Shimizu-Yumoto H (2009). Recent breakthroughs in postharvest physiology research and cut flower handling in Japan. Horticulture Environment and Biotechnology , 50(6): 539–545
19 Jones M L, Chaffin G S, Eason J R, Clark D G (2005). Ethylene-sensitivity regulates proteolytic activity and cysteine protease gene expression in Petunia corollas. J Exp Bot , 56(420): 2733–2744
doi: 10.1093/jxb/eri266 pmid:16131506
20 Jones M L, Larsen P B, Woodson W R (1995). Ethylene-regulated expression of a carnation cysteine proteinase during flower petal senescence. Plant Mol Biol , 28(3): 505–512
doi: 10.1007/BF00020397 pmid:7632919
21 Lay-Yee M, Stead A D, Reid M S (1992). Flower senescence in daylily (Hemerocallis). Physiol Plant , 86(2): 308–314
doi: 10.1034/j.1399-3054.1992.860218.x
22 Lerslerwong L, Ketsa S, van Doorn W G (2009). Protein degradation and peptidase activity during petal senescence in Dendrobium cv. Khao sanan. Postharvest Biol Technol , 52(1): 84–90
doi: 10.1016/j.postharvbio.2008.09.009
23 Lowry O H, Rosebrough N J, Farr A L, Randall R J (1951). Protein measurement with the Folin phenol reagent. J Biol Chem , 193(1): 265–275
pmid:14907713
24 Nelson N (1944). A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem , 153: 375–380
25 Rogers H J (2006). Programmed cell death in floral organs: how and why do flowers die? Ann Bot (Lond) , 97(3): 309–315
doi: 10.1093/aob/mcj051 pmid:16394024
26 Rosen H (1957). A modified ninhydrin colorimetric analysis for amino acids. Arch Biochem Biophys , 67(1): 10–15
doi: 10.1016/0003-9861(57)90241-2 pmid:13412116
27 Rubinstein B (2000). Regulation of cell death in flower petals. Plant Mol Biol , 44(3): 303–318
doi: 10.1023/A:1026540524990 pmid:11199390
28 Solomon M, Belenghi B, Delledonne M, Menachem E, Levine A (1999). The involvement of cysteine proteases and protease inhibitor genes in the regulation of programmed cell death in plants. Plant Cell , 11(3): 431–444
pmid:10072402
29 Swain T, Hillis W E (1959). The phenolic constituents of Prunus domestica I.— The quantitative analysis of phenolic constituents. J Sci Fd Agr , 10(1): 63–68
doi: 10.1002/jsfa.2740100110
30 Tayyab S, Qamar S (1992). A look into enzyme kinetics: Some introductory experiments. Biochem Edu , 20(2): 116–118
doi: 10.1016/0307-4412(92)90122-3
31 Tripathi S K, Singh A P, Sane A P, Nath P (2009). Transcriptional activation of a 37 kDa ethylene responsive cysteine protease gene, RbCP1, is associated with protein degradation during petal abscission in rose. J Exp Bot , 60(7): 2035–2044
doi: 10.1093/jxb/erp076 pmid:19346241
32 Tripathi S K, Tuteja N (2007). Integrated signalling in flower senescence: an overview. Plant Signal Behav , 2(6): 437–445
pmid:19517004
33 Trobacher C P (2009). Ethylene and programmed cell death in plants. Botany , 87(8): 757–769
doi: 10.1139/B09-041
34 van Doorn W G (2001). Categories of petal senescence and abscission: a re-evaluation. Ann Bot (Lond) , 87(4): 447–456
doi: 10.1006/anbo.2000.1357
35 van Doorn W G (2004). Is petal senescence due to sugar starvation? Plant Physiol , 134(1): 35–42
doi: 10.1104/pp.103.033084 pmid:14730063
36 van Doorn W G, Groenewegen G, van de Pol P, Berkholst E M (1991). Effects of carbohydrate and water status on flower opening of cut Madelon roses. Postharvest Biol Technol , 1(1): 47–57
doi: 10.1016/0925-5214(91)90018-7
37 van Doorn W G, Woltering E J (2008). Physiology and molecular biology of petal senescence. J Exp Bot , 59(3): 453–480
doi: 10.1093/jxb/erm356 pmid:18310084
38 Wagstaff C, Leverentz M K, Griffiths G, Thomas B, Chanasut U, Stead A D, Rogers H J (2002). Cysteine protease gene expression and proteolytic activity during senescence of Alstroemeria petals. J Exp Bot , 53(367): 233–240
doi: 10.1093/jexbot/53.367.233 pmid:11807127
39 Woltering E J, de Jong A, Hoeberichts F A, lakimova E, Kapchina V (2005). Plant programmed cell death, ethylene and flower senescence. Acta Hortic , 669: 159–170
40 Woltering E J, van Doorn W G (1988). Role of ethylene in senescence of petals- morphological and taxonomic relationships. J Exp Bot , 39(11): 1605–1616
doi: 10.1093/jxb/39.11.1605
41 Woodson W R, Handa A K (1987). Changes in protein patterns and in vivo protein synthesis during presenescence and senescence of hibiscus petals. J Plant Physiol , 128(1-2): 67–75
42 Yamada K, Ito M, Oyama T, Nakada M, Maesaka M, Yamaki S (2007). Analysis of sucrose metabolism during petal growth of cut roses. Postharvest Biol Technol , 43(1): 174–177
doi: 10.1016/j.postharvbio.2006.08.009
43 Zhou Y, Wang C Y, Ge H, Hoeberichts F A, Visser P B (2005). Programmed cell death in relation to petal senescence in ornamental plants. J Integr Plant Biol , 47(6): 641–650
doi: 10.1111/j.1744-7909.2005.00112.x
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