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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

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Front Agric Chin    2011, Vol. 5 Issue (1) : 60-63    https://doi.org/10.1007/s11703-011-1068-4
RESEARCH ARTICLE
Bacterial populations on the phyllosphere of Mediterranean plants: influence of leaf age and leaf surface
Ram Kailash P. YADAV1(), Katerina KARAMANOLI2, Despoina VOKOU3
1. Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal; 2. Laboratory of Agricultural Chemistry, School of Agriculture, Aristotle University, Gr-541 24 Thessaloniki, Greece; 3. Department of Ecology, School of Biology, Aristotle University, Gr-541 24 Thessaloniki, Greece
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Abstract

In the present study, we estimated the size of phyllosphere bacterial populations in young and mature leaves from the same plants and also assessed the population abundance on adaxial and abaxial leaf surfaces. We examined eight perennial species naturally occurring in the same area, in Halkidiki (northern Greece). They are BoldItalic and BoldItalic (evergreen sclerophyllous species), BoldItalic and BoldItalic (drought semideciduous species), and BoldItalic and BoldItalic (nonwoody perennial species). Young and mature leaves were examined from the four sclerophyllous evergreen species for their epiphytic bacterial colonization, and it was found that mature leaves were highly populated compared to the younger ones except in BoldItalic. As regards the bacterial colonization of the two leaf surfaces, no differences were found in most species except for the drought semideciduous type where the two leaf surfaces behaved differently.
Keywords bacterial population      phyllosphere      leaf surface      leaf age      leaf imprints      Mediterranean plants     
Corresponding Author(s): YADAV Ram Kailash P.,Email:rkp.yadav@cdbtu.edu.np   
Issue Date: 05 March 2011
 Cite this article:   
Ram Kailash P. YADAV,Katerina KARAMANOLI,Despoina VOKOU. Bacterial populations on the phyllosphere of Mediterranean plants: influence of leaf age and leaf surface[J]. Front Agric Chin, 2011, 5(1): 60-63.
 URL:  
https://academic.hep.com.cn/fag/EN/10.1007/s11703-011-1068-4
https://academic.hep.com.cn/fag/EN/Y2011/V5/I1/60
Fig.1  Total bacterial population of young and mature leaves
Note: Columns represent mean bacterial population ( = 16) and error bars represent SE of the means. Significant differences between young and mature leaf populations are indicated by * (<0.05) or ** (<0.01).
source of variationSSdfFP
species6.3034.760.004
age9.39121.26&lt;0.001
species×age3.9933.010.033
error52.99120——
Tab.1  Results of two-way ANOVA of the total bacterial population of young and mature leaves of four evergreen sclerophyllous species Significant -values are indicated in column
Fig.2  Bacterial population on the adaxial and abaxial leaf surfaces
Note: Vertical bars represent means and the error bars represent SE of the means ( = 6). Asterisks indicate significant differences at <0.05.
source of variationSSdfFP
species5.88710.46&lt;0.001
surface0.1511.880.174
species×surface1.4072.480.023
error6.4380——
Tab.2  Results of two-way ANOVA of bacterial populations on the adaxial and abaxial leaf surfaces of eight Mediterranean species
1 Andrews J H, Kenerley C M (1980). Microbial populations associated with buds and young leaves of apple. Can J Bot , 58: 847-855
2 Aneja K R (2003). Experiments in Microbiology, Plant Pathology and Biotechnology. New Delhi: New Age International (P) Ltd. 178
3 Berger C N, Sodha S V, Shaw R K, Griffin P M, Pink D, Hand P, Frankel G (2010). Fresh fruit and vegetables as vehicles for the transmission of human pathogens. Environ Microbiol , 12(9): 2385-2397
doi: 10.1111/j.1462-2920.2010.02297.x pmid:20636374
4 de Jager E S, Wehner F C, Korsten L (2001). Microbial ecology of the mango phylloplane. Microb Ecol , 42(2): 201-207
pmid:12024283
5 Dickinson C H, Austin B, Goodfellow M (1975). Quantitative and qualitative studies of phylloplane bacteria from Lollium perenne. J Gen Microbiol , 91: 157-166
6 Ercolani G L (1976). Bacteriological quality assessment of fresh marketed lettuce and fennel. Appl Environ Microbiol , 31(6): 847-852
pmid:820256
7 Ercolani G L (1991). Distribution of epiphytic bacteria on olive leaves and the influence of leaf age and sampling time. Microb Ecol , 21(1): 35-48
doi: 10.1007/BF02539143
8 Gau A E, Dietrich C, Kloppstech K (2002). Non-invasive determination of plant-associated bacteria in the phyllosphere of plants. Environ Microbiol , 4(11): 744-753
doi: 10.1046/j.1462-2920.2002.00359.x pmid:12460282
9 Geeson J D (1979). The fungal and bacterial flora of stored white cabbage. J Appl Bacteriol , 46(1): 189-193
pmid:438106
10 Hallam N D, Juniper B E (1971). The anatomy of the leaf surface. In: Preece T F, Dickinson C H, eds. Ecology of Leaf Surface Micro-organisms . London: Academic Press, 3-37
11 Herms D A, Mattson W J (1992). The dilemma of plants: to grow or defend. Q Rev Biol , 67(3): 283–335
doi: 10.1086/417659
12 Jacques M A, Kinkel L L, Morris C E (1995). Population sizes, immigration, and growth of epiphytic bacteria on leaves of different ages and positions of field-grown endive (Cichorium endivia var. latifolia). Appl Environ Microbiol , 61(3): 899-906
pmid:16534973
13 Jones J B, Chase A R, Harbaugh B K, Raju B C (1985). Effect of leaf wetness, fertilizer rate, leaf age and light intensity before inoculation on bacterial leaf spot of Chrysanthemum. Plant Dis , 69: 782-784
14 Juniper B E (1991). The leaf from the inside and the outside: a microbe’s point of view. In: Andrews J H, Hirano S S, eds. Microbial Ecology of Leaves . New York Inc: Springer-Verlag, 21-42
15 Kinkel L, Wilson M, Lindow S E (1995). Effect of sampling scale on the assessment of epiphytic bacterial populations. Microb Ecol , 29(3): 283-297
doi: 10.1007/BF00164891
16 Lambais M R, Crowley D E, Cury J C, Büll R C, Rodrigues R R (2006). Bacterial diversity in tree canopies of the Atlantic forest. Science , 312(5782): 1917
doi: 10.1126/science.1124696 pmid:16809531
17 Lindow S E, Arny D C, Upper C D (1978). Distribution of ice nucleation-active bacteria on plants in nature. Appl Environ Microbiol , 36(6): 831-838
pmid:736541
18 Mansvelt E L, Hattingh M J (1987). Scanning electron microscopy of colonization of pear leaves by Pseudomonas syringae pv. syringae. Can J Bot , 65(12): 2517-2522
doi: 10.1139/b87-341
19 Morris C E, Kinkel L (2002). Fifty years of phyllosphere microbiology: significant contributions to research in related fields. In: Lindow S E, Hecht-Poinar E I, Elliott V J, eds. Phyllosphere Microbiology . Minnesota: APS Press, 365-375
20 Morris C E, Lucotte T (1993). Dynamics and variability of bacterial population density on leaves of field-grown endive destined for ready-to use processing. Int J Food Sci Technol , 28(2): 201-209
doi: 10.1111/j.1365-2621.1993.tb01265.x
21 Périssol C, Roux M, Le Petit J (1993). Succession of bacteria attached to evergreen oak leaf surfaces. Eur J Soil Biol , 29: 167-176
22 Surico G (1993). Scanning electron microscopy of olive leaves colonized by Pseudomonas syringae subsp. savastanoi. J Phytopathol , 138(1): 31-40
doi: 10.1111/j.1439-0434.1993.tb01358.x
23 Thompson I P, Bailey M J, Fenlon J S, Fermor T R, Lilley A K, Lynch J M, McCormack P J, Mc Quilken M P, Purdy R J, Rainey P B, Whipps J M (1993). Quantitative and qualitative seasonal changes in the microbial community from the phyllosphere of sugar beet (Beta vulgaris). Plant Soil , 150(2): 177-191
doi: 10.1007/BF00013015
24 Tukey H B Jr (1970). The leaching of substances from plants. Annu Rev Plant Physiol , 21(1): 305-324
doi: 10.1146/annurev.pp.21.060170.001513
25 Van Outryve M F, Gosselé F, Swings J (1989). The bacterial microflora of witloof cichory (Cichorium intybus L. var. foliosum Hegi) leaves. Microb Ecol , 18(2): 175-186
doi: 10.1007/BF02030125
26 Weller D M, Saettler A W (1980). Colonization and distribution of Xanthomonas phaseoli and X. phaseoli var. fuscans in field-grown navy beans. Phytopathology , 70(6): 500-506
doi: 10.1094/Phyto-70-500
27 Whipps J M, Hand P, Pink D A C, Bending G D (2008). Human pathogens and the phyllosphere. Adv Appl Microbiol , 64: 183-221
doi: 10.1016/S0065-2164(08)00407-3 pmid:18485286
28 Yadav R K P, Bosabalidis A M, Vokou D (2004a). Leaf structural features of Mediterranean perennial species: plasticity and life form specificity. J Biol Res , 2: 21-34
29 Yadav R K P, Halley J M, Karamanoli K, Constantinidou H I, Vokou D (2004). Bacterial populations on the leaves of Mediterranean plants: quantitative features and testing of distribution models. Environ Exp Bot , 52(1): 63-77
doi: 10.1016/j.envexpbot.2004.01.004
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