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Cloning and pharmaceutical analysis of CaMK gene of Botrytis cinerea |
Yuxia DONG1, Jihong XING1, Jiao JIA1, Qiaoyun WENG2, Zhimin HAO1, Jingao DONG1() |
1. Molecular Plant Pathology Lab, Agricultural University of Hebei, Baoding 071001, China; 2. Department of Agricultural and Forest Technology, Hebei North University, Zhangjiakou 075131, China |
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Abstract Using a PCR homology approach, DNA and cDNA sequences of calcium/calmodulin-dependent protein kinase (CaMK) gene of Botrytis cinerea were obtained. Southern blotting result displayed that CaMK was single copy in the genome of B. cinerea. The cDNA sequence of CaMK revealed an open reading frame of 2190 nucleotides encoding a 730 amino acid protein with predicted molecular weight of 81.8748 kDa. The genomic sequence of CaMK revealed the same ORF interrupted by six introns. Bioinformatics analysis showed that this protein had the distinctive features that characterize CaMK ATP binding region signature and serine/threonine protein kinase active-site signature. Pharmaceutical analysis displayed that the CaMK specific inhibitor, KN-62, could inhibit conidial germination, pathogenicity and herbicidal activity of B. cinerea BC4 strain. It was suggested that CaMK played an important role in regulating conidial germination, pathogenicity and herbicidal activity of B. cinerea.
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Keywords
Botrytis cinerea
CaMK
pharmaceutical analysis
KN-62
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Corresponding Author(s):
DONG Jingao,Email:shmdjg@hebau.edu.cn
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Issue Date: 05 September 2011
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