Development of EST-SSR markers in peanut (<italic>Arachis hypogaea</italic> L.)

doi:10.1007/s11703-011-1091-5

Front Agric Chin

2011, Vol. 5

Issue (3) : 268-273 https://doi.org/10.1007/s11703-011-1091-5

RESEARCH ARTICLE

Development of EST-SSR markers in peanut (Arachis hypogaea L.)

Xinyan WEI, Lifeng LIU(

), Shunli CUI, Huanying CHEN, Jingjing ZHANG

Agricultural University of Hebei, Baoding 071001, China

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Abstract

More molecular markers for potential use in peanut genetic research were developed. A total of 92403 EST sequences of peanut (Arachis) in the NCBI database were downloaded and analyzed. 2594 SSRs distributed in 2267 non-redundant EST sequences were detected, with tri-nucleotide motif (65.54%) as the most abundant motif type followed by di-nucleotide motif (28.10%). Among the 92 repeat types, the top eight motif types were AG/TC (20.1%), AAG/TTC (11.8%), AAT/TTA (10.1%), AGG/TCC (6.6%), AGA/TCT (6.3%), AT/TA (5.9%), ACT/TGA (3.8%), and ATG/TAC (3.7%) with higher frequency. A total of 237 primer pairs were successfully designed based on the 2267 SSR-ESTs using DNA star software.

Keywords peanut EST-SSR development

Corresponding Author(s): LIU Lifeng,Email:Liulifeng@hebau.edu.cn

Issue Date: 05 September 2011

Cite this article:

Xinyan WEI,Lifeng LIU,Shunli CUI, et al. Development of EST-SSR markers in peanut (Arachis hypogaea L.)[J]. Front Agric Chin, 2011, 5(3): 268-273.

URL:

https://academic.hep.com.cn/fag/EN/10.1007/s11703-011-1091-5
https://academic.hep.com.cn/fag/EN/Y2011/V5/I3/268

Tab.1 Occurrence of SSRs in non-redundant peanut ESTs

Tab.2 Occurrence of different SSR of peanut

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