Genetic diversity studies of coarse and fine rice using RAPD markers

doi:10.1007/s11703-011-1106-2

Front Agric Chin

2011, Vol. 5

Issue (2) : 129-134 https://doi.org/10.1007/s11703-011-1106-2

RESEARCH ARTICLE

Genetic diversity studies of coarse and fine rice using RAPD markers

Saeed ARSHAD, Ahsan IQBAL, Sehar NAWAZ, Nisar AHMED(

)

Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad 38040, Pakistan

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Abstract

The availability of a genetically diverse gene pool is vitally important in varietal development. Molecular markers are being extensively utilized to explore the genetic diversity among native and exotic germplasm. This study was designed to reveal the genetic diversity and patterns of relationships among the 20 accessions/genotypes representative of basmati and non-basmati rice from the existing rice gene pool using RAPD markers. Employing RAPD, 17 decamer oligonucleotide primers directed the amplification of 116 fragments, out of which 101 were polymorphic (87.06%) while 15 fragments were monomorphic (12.93%). Similarity coefficients had ranged from 0.47 to 0.90. The average genetic similarity was calculated 0.68 (68%). In this study, the coarse rice genotypes showed more polymorphism (85.84%) than the fine rice genotypes (61.76%). Genotypes were clustered into 8 distinct groups: A, B, C, D, E, F, G, and H but two genotypes, i.e., Shadab and Kangni-27 showed divergence from all the genotypes of the groups. Therefore, these diverse genotypes may be included in future breeding programmes.

Keywords RAPD diversity studies genetic markers fine rice coarse rice

Corresponding Author(s): AHMED Nisar,Email:drmiannisar@yahoo.com

Issue Date: 05 June 2011

Cite this article:

Saeed ARSHAD,Ahsan IQBAL,Sehar NAWAZ, et al. Genetic diversity studies of coarse and fine rice using RAPD markers[J]. Front Agric Chin, 2011, 5(2): 129-134.

URL:

https://academic.hep.com.cn/fag/EN/10.1007/s11703-011-1106-2
https://academic.hep.com.cn/fag/EN/Y2011/V5/I2/129

Fig.1 Amplification product of RAPD primer J-20
Note: M= 1 kb DNA ladder. Fine rice genotypes: 1= sugdasi-sadagulab, 2= basmati-217, 3= punjab-basmati, 4= basmati-385, 5= rachna-basmati, 6= dhera-dun-basmati, 7= dokri-basmati, 8= kashmir-basmati, 9= khushboo-95, 10= lateefy; Coarse rice genotypes: 11= sathra, 12= shadab, 13= shua-92, 14= mahlar-346, 15= ir-8, 16= kasalath, 17= kharai-ganga, 18= sonahri-kangni, 19= jhona-349, 20= kangni-27. The same as below.

Fig.2 Amplification product of RAPD primer C-11

Fig.3 Dendrogram of 20 (20) rice varieties obtained from similarity matrix based on Nie’s UPGMA RAPD

Tab.1 Table 1 Similarity matrix of twenty coarse and fine rice genotypes

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