Identification and molecular tagging of two <italic>Arabidopsis</italic> resistance genes to <italic>Botrytis cinerea

doi:10.1007/s11703-011-1107-1

Front Agric Chin

2011, Vol. 5

Issue (4) : 430-436 https://doi.org/10.1007/s11703-011-1107-1

RESEARCH ARTICLE

Identification and molecular tagging of two Arabidopsis resistance genes to Botrytis cinerea

Jihong XING¹, Qiaoyun WENG², Helong SI¹, Jianmin HAN¹, Jingao DONG¹(

)

1. Molecular Plant Pathology Lab, College of Life Science, Agricultural University of Hebei, Baoding 071001, China; 2. Department of Agricultural and Forest Technology, Hebei North University, Zhangjiakou 075131, China

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Abstract

To map Arabidopsis resistance genes to Botrytis cinerea, Arabidopsis Col-0 ecotype resistant to B. cinerea BC18 isolate and Arabidopsis Ler ecotype susceptible to B. cinerea BC18 isolate were crossed. According to the resistant responses of the F₁, BC₁ and F₂ populations to B. cinerea, we identified two genes, named BC1 and BC2, responsible for the resistance of Arabidopsis Ler ecotype to B. cinerea. Through the method of map-based cloning, BC1 was linked to DNA markers CCR1 and DHS1 on the fourth chromosome of Arabidopsis with genetic distances of 1.2 cM and 1.6 cM for CCR1 and DHS1, respectively, and BC2 was linked to DNA markers CA72/NGA151 and NGA106 on the fifth chromosome with genetic distances of 1.4 cM and 2.4 cM for CA72/NGA151 and NGA106, respectively. Our results are beneficial for chromosome walking so that we can obtain the whole gene sequences, which will facilitate the understanding of their roles and manners of resistance to B. cinerea.

Keywords Arabidopsis thaliana Botrytis cinerea resistant genes linkage analysis gene mapping

Corresponding Author(s): DONG Jingao,Email:shmdjg@hebau.edu.cn

Issue Date: 05 December 2011

Cite this article:

Jihong XING,Qiaoyun WENG,Helong SI, et al. Identification and molecular tagging of two Arabidopsis resistance genes to Botrytis cinerea[J]. Front Agric Chin, 2011, 5(4): 430-436.

URL:

https://academic.hep.com.cn/fag/EN/10.1007/s11703-011-1107-1
https://academic.hep.com.cn/fag/EN/Y2011/V5/I4/430

Tab.1 SSLP markers used in this study

Fig.1 Morphologic comparison between hybrid plant and parents.

Fig.2 Results of PCR amplification using SSLP primers located on different chromosomes.

Fig.3 Results of PCR amplification with primers NGA172 and NGA8. Col means male parent; L means female parent; * means non-hybrid plant.

Fig.4 Choice of linkage markers. Col means male parent; L means female parent; F means F hybrid plants; means resistance plant pool and means susceptible plant pool.

Fig.5 The segregation of partial polymorphic markers on the F Populations. Col means male parent and L means female parent.

Fig.6 Linkage map of the locus on chromosomes.

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