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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2011, Vol. 5 Issue (3) : 305-309     DOI: 10.1007/s11703-011-1116-0
RESEARCH ARTICLE |
The control effect of a multifunctional bacterial agent fit for straw amendment against wheat soil-borne diseases
Shan LI1, Yongsheng ZHANG2, Yanan WANG1,3, Chunqi LIANG1, Wenchao ZHEN1,3()
1. College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China; 2. College of Agronomy, Agricultural University of Hebei, Baoding 071001, China; 3. Research Center for Biocontrol Techniques against Pests on Crops of Hebei Province, Baoding 071001, China
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Abstract  

Soil-borne diseases of wheat are getting more and more serious in the wheat/maize rotation growing system in northern China. A multifunctional microorganic strain called B1514 was found to have an inhibitory effect against major pathogens of winter wheat soil-borne diseases, have the ability to decompose maize straw, and have the ability to utilize the straw for multiplication. The strain was processed into bacterial agent HAD-1. Field experiments were conducted from 2008 to 2010 to test the control effect of HAD-1 on the major wheat soil-borne diseases, on decomposing ability to maize straw, and on reproductive capacity. Results showed that HAD-1 had significant control effects on sharp eyespot, take-all, and root rot on wheat. The control efficacy at wheat jointing stage was 59.63% to 72.59%, 57.64% to 59.29%, and 54.48% to 63.25%, respectively. The yield loss decreased by 8.67% to 11.70%. The population numbers of the strain B1514 increased 2.68×107–4.83×107 times during the wheat growing season. HAD-1 significantly accelerated the decomposition rate of maize straw in the soil. The decomposition rate increased by 18.7% to 24.3% during wheat growing season.

Keywords Straw amendment      bacterial agent      winter wheat      soil-borne disease      bio-control     
Corresponding Authors: ZHEN Wenchao,Email:wenchao@hebau.edu.cn   
Issue Date: 05 September 2011
URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-011-1116-0     OR     http://academic.hep.com.cn/fag/EN/Y2011/V5/I3/305
YearStagesTreatmentsTake-allSharp eyespotRoot rot
Disease indexControl efficacy (%)Disease indexControl efficacy (%)Disease indexControl efficacy (%)
2008-2009Pre-overwinteringHAD-13.76a51.303.47a78.045.62a64.63
Control7.72b15.80b15.89b
JointingHAD-13.90a72.5912.03a57.6410.36a63.25
Control14.23b28.40b28.19b
FillingHAD-15.24a79.998.63a68.038.20a70.51
Control26.19b26.99b27.81b
2009-2010Pre-overwinteringHAD-107.4a48.475.34a69.667.12a53.49
Control14.36b17.60b15.31b
JointingHAD-16.62a59.637.78a59.297.32a54.48
Control16.4b19.11b16.08b
FillingHAD-16.18a64.7910.80a61.0410.16a59.42
Control17.55b27.72b25.04b
Tab.1  Control effect of the bacterial agent HAD-1 to three wheat soil-borne diseases in the field
YearPathogenTreatmentSpike No. (million/hm2)Grain No. (number/spike)1000-grain weight (g)Grain yield (kg/hm2)Yield loss rate (%)
2008-2009G. graminisHAD-1652.9a35.8a41.6a8265.4a11.70
CK1630.3b35.0b38.4b7211.8b
R. cerealisHAD-1708.1a34.8a41.4a8669.0a9.40
CK1612.0b33.6b44.8a7821.5b
B. sorokinianaHAD-1643.7a35.5a43.3a8406.4a10.35
Control638.5a34.8b39.5b7474.1b
CK2untreated720.836.640.19008.5
2009-2010Mix pathogenHAD-1692.3a39.0a38.8a8236.0a8.67
CK1605.4b38.1b37.9b7804.7b
CK2untreated670.238.339.18426.5
Tab.2  Effect of application of bacterial agent HAD-1 on wheat yield
YearPathogenProliferation rate of B1514 (times)
Sowing to pre-winterOverwintering stageGreenup to jointing stageJointing to maturity stageGrowing season
2008-2009R. cerealis102.3827.46112.20122.363.86×107
B. graminis98.9619.82103.34132.462.68×107
G. graminis104.8832.49110.02128.944.83×107
2009-2010Mixed pathogens101.1033.45107.49120.314.37×107
Tab.3  Proliferation rates of multifunctional strain B1514 at different stages of wheat
YearPathogenTreatmentDegradation rate (%)
Sowing to pre-winterOver wintering stageGreen up to jointing stageJointing to maturity stageGrowing season
2008-2009R. cerealisHAD-111.97a3.88a11.76a14.77a42.38a
CK16.72b2.32b5.93b6.52b21.49b
B. sorokinianaHAD-112.72a3.87a10.87a16.06a43.52a
CK15.95b2.61b6.47b9.55b24.58b
G. graminisHAD-112.56a3.02a12.36a11.99a39.93a
CK16.83b2.05b7.43b7.33b23.64b
2009-2010Mixed pathogensHAD-112.42a3.59a11.66a14.27a41.94a
CK16.50b2.33b6.61b7.80b23.24b
Tab.4  Degradation rate of the multifunctional bacterial agent HAD-1 to maize straw
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