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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2011, Vol. 5 Issue (3) : 382-387     DOI: 10.1007/s11703-011-1118-y
Effect of dry and wet storage at cool temperatures on the postharvest performance of Ranunculus asiaticus L. flowers
Waseem SHAHRI(), Inayatullah TAHIR, Sheikh Tajamul ISLAM, Mushtaq Ahmad BHAT
Plant Physiology and Biochemistry Research Laboratory, Department of Botany, University of Kashmir, Srinagar 190006, India
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A study was undertaken to assess the effect of different storage temperatures on senescence and postharvest performance of isolated flowers of Ranunculus asiaticus L.. The main aim of the work was to develop a cost-effective storage protocol to reduce the postharvest losses and to bring out the transportation of cut flowers of R. asiaticus. The flowers were subjected to two different storage treatments, dry storage and wet storage, and their postharvest performance was compared under laboratory conditions. For this purpose, the buds were harvested at 8:00 AM at loose bud stage (Stage II of flower development). The harvested buds were cut to a uniform size of 15 cm and processed for dry or wet storage. For dry storage, the buds were packed after wrapping them in moistened filter papers and kept at 5oC and 10oC. For wet storage, the buds were held in distilled water in separate glass beakers kept at 5oC and 10oC, respectively. A separate set of buds each for dry and wet storage was kept at room temperature (15±2)oC. After 72 h storage, the buds were kept at room temperature in distilled water. The average life of an individual flower that opened fully was about 4–5 days. The buds kept under wet storage at 5oC and 10oC for 72 h maintained their premature status, while the buds held at room temperature for 72 h generally bloomed. All the buds stored dry maintained their premature status irrespective of storage temperature. Storage of buds for 72 h at 5oC, followed by transferring to distilled water improved the longevity by about 5 to 6 days. Cold storage treatment before transferring to holding solution improved floral diameter, membrane integrity besides maintaining higher fresh and dry mass of flowers, sugar content, soluble proteins, and phenols. Our results suggested that wet and dry storage of premature buds of R. asiaticus for 72 h at 5oC, followed by placing them in distilled water, improved the cut flower longevity and can be used as effective postharvest storage treatments for this beautiful cut flower.

Keywords longevity      sugars      phenols      cold storage      membrane permeability     
Corresponding Authors: SHAHRI Waseem,   
Issue Date: 05 September 2011
URL:     OR
Fig.1  Effect of dry and wet storage for 72 h at different storage temperatures [Room temperature, RT ((15±2)oC), 10oC and 5oC] on different traits of flowers. Figures 1A–J represent vase life, solution uptake, conductivity of leachates, floral diameter, fresh mass, dry mass, sugar fractions (1G and 1H), soluble proteins, and total phenols at day 6 after transferring to (DW) in isolated flowers of , respectively. LSD is computed at .
Fig.2  Flower buds of before (A) and after (B) 72 h postharvest dry storage at different temperature regimes. From left to right, the arrangement buds kept at room temperature (RT, (15±2)oC), 10oC, and 5oC is shown.
Fig.3  Flower buds of held in glass beakers containing distilled water (DW). Figures 3A and 3B are carried out before and after 72 h postharvest on wet storage at different temperature regimes, respectively. From left to right, the arrangement of buds kept at room temperature (RT, (15±2)oC), 10oC, and 5oC is shown.
Fig.4  Flowers of held in distilled water (DW) after 72 h dry storage at different days after transfer to DW. Figures 4A–C represent days 2, 4, and 6 after transfer (Fig. C). From left to right, the arrangement of the flasks containing buds previously held at different temperatures, namely, (RT, (15±2)oC), 10oC and 5oC is shown.
Fig.5  Flowers of held in distilled water (DW) after 72 h wet storage at different days after transfer to DW. Figures 5A–C represent days 2, 4, and 6, respectively, after transfer. From left to right, the arrangement of the flasks containing buds previously held at different temperatures, namely, (RT, (15±2)°C), 10°C and 5°C.
1 Ahn G Y, Park J C (1996). Effect of harvest time on quality preservation of cut rose ‘Mary de Vor’. Journal of Korean Society for Horticultural Science , 37: 603–606
2 Bunya-atichart K, Ketsa S, Van Doorn W G (2004). Postharvest physiology of curcuma alismatifolia flowers. Postharvest Biology and Technology, 34(2): 219–226
doi: 10.1016/j.postharvbio.2004.05.009
3 Celikel F G, Reid M S (2002). Storage temperature affects the quality of cut flowers from the Asteraceae. HortScience , 37(1): 148–150
4 Cevallos J C, Reid M S (2001). Effect of dry and wet storage at different temperatures on the vase life of cut flowers. HortTechnology , 11: 199–202
5 Gul F, Tahir I (2009). Effect of cool and wet storage on postharvest performance of Nerine sarniensis cv. Red scapes. Acta Horticulturae , 847: 345–352
6 Gul F, Tahir I, Sultan S M (2007). Effect of storage temperature on postharvest performance of Amaryllis belladonna L. cv. Rosea scapes. Journal of Plant Biology , 34: 43–47
7 Ichimura K, Kojima K, Goto R (1999). Effects of temperature, 8-hydroxyquinoline sulphate and sucrose on the vase life of cut rose flowers. Postharvest Biology and Technology , 15(1): 33–40
doi: 10.1016/S0925-5214(98)00063-5
8 Jones M L (2008). Ethylene signaling is required for pollination-accelerated corolla senescence in Petunias. Plant Science , 175(1-2): 190–196
doi: 10.1016/j.plantsci.2008.03.011
9 Joyce D C, Meara S A, Hetherington S E, Jones P (2000). Effects of cold storage on cut Grevillea ‘Sylvia’ inflorescences. Postharvest Biology and Technology , 18(1): 49–56
doi: 10.1016/S0925-5214(99)00059-9
10 Lee A, Suh J (1996). Effect of harvest stage, pre- and post-harvest treatment on longevity of cut Lilium flowers. Acta Horticulturae , 414: 287–294
11 Leonard R T, Nell T A, Suzuki A, Barrett J E, Clark D G (2001). Evaluation of long term transport of Colombian grown cut roses. Acta Horticulturae , 543: 285–291
12 Lowry O H, Rosebrough N J, Farr A L, Randall R J (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry , 193(1): 265–275
13 Mwangi M, Chatterjee S R, Bhattacharjee S K (2003). Changes in the biochemical constituents of “Golden gate” cut rose petals as affected by precooling with ice cold water spray, pulsing and packaging. Journal of Plant Biology , 30: 95–97
14 Nelson N (1944). Photometric adaptation of Somogy’s method for the determination of glucose. J Biol Chem , 153: 375–380
15 Nowak J, Rudnicki R M (1990). Storage. In: Nowak J, Rudnicki R M, eds. Postharvest Handling and Storage of Cut Flowers, Florist Greens and Potted Plants. Portland: Timber Press, 67–86
16 Page T, Griffiths G, Buchanan-Wollaston V (2001). Molecular and biochemical characterization of postharvest senescence in broccoli. Plant Physiol , 125(2): 718–727
doi: 10.1104/pp.125.2.718 pmid:11161029
17 Redman P B, Dole J M, Maness N O, Anderson J A (2002). Post harvest handling of nine specialty cut flower species. Scientia Horticulturae , 92(3-4): 293–303
doi: 10.1016/S0304-4238(01)00294-1
18 Shahri W, Tahir I, Gul F (2009). Effect of dry and wet storage at cool temperatures on postharvest performance of Consolida ajacis cv. Violet blue spikes. Journal of Plant Biology , 36: 5–9
19 Swain T, Hillis W E (1959). The phenolic constituents of Prunus domestica I.—the quantitative analysis of phenolic constituents. Journal of the Science of Food and Agriculture , 10(1): 63–68
doi: 10.1002/jsfa.2740100110
20 Taverner E, Letham D S, Wang J, Cornish E, Wilkins D A (1999). Influence of ethylene on cytokinin metabolism in relation to Petunia corolla senescence. Phytochemistry , 51(3): 341–347
doi: 10.1016/S0031-9422(98)00757-2
21 van der Meulen-Muisers J J M, van Oeveren J C (1997). Influence of bulb stock origin, inflorescence harvest stage and postharvest evaluation conditions on cut flower longevity of Asiatic hybrid lilies. Journal of the American Society for Horticultural Science , 122: 368–372
22 van Doorn W G, de Witte Y (1991). Effect of dry storage on bacterial counts in stems of cut rose flowers. HortScience, 26 : 1521–1522
23 van Doorn W G, Woltering E J (2005). Many ways to exit? Cell death categories in plants. Trends in Plant Science , 10(3): 117–122
doi: 10.1016/j.tplants.2005.01.006 pmid:15749469
24 Wagstaff C, Chanasut U, Harren F J M, Laarhoven L J, Thomas B, Rogers H J, Stead A D (2005). Ethylene and flower longevity in Alstroemeria: relationship between tepal senescence, abscission and ethylene biosynthesis. Journal of Experimental Botany , 56(413): 1007–1016
doi: 10.1093/jxb/eri094 pmid:15689338
25 Zhou Y, Wang C Y, Cheng Z W (2008). Effect of exogenous ethylene and ethylene inhibitor on longevity and petal senescence of sweet Osmanthus. Acta Horticulturae , 768: 487–493
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