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Construction of a Fosmid genomic library of Streptomyces roseoflavus Men-myco-93-63 |
Weiming SUN1,2, Wei GUO1,2,3(), Daqun LIU1(), Tinghui LIU1, Lina FENG1, Lianna LIU2, Yaning LI1, Yakun ZHANG2 |
1. Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, College of Plant Protection, Agricultural University of Hebei, Baoding 071000, China; 2. College of Life Sciences, Agricultural University of Hebei, Baoding 071000, China; 3. National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding 071000, China |
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Abstract To clone the antibiotic biosynthesis gene cluster of Streptomyces roseoflavus Men-myco-93-63, we constructed a Fosmid genomic library. The genomic DNA of the strain Men-myco-93-63 was isolated by the modified CTAB procedure, and the size of most genomic DNA fragments was larger than 150 kb. Then, a Fosmid genomic library containing more than 6000 clones was constructed. The average size of the inserted DNA in recombinant plasmids was 38.1 kb, and the probability of harboring any gene in the genome of the strain Men-myco-93-63 was 99.99%. The library coverage was at least a 10-fold genome equivalent. Therefore, the constructed Fosmid library meets the requirements as a standard genomic library
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Keywords
Streptomyces roseoflavus
Fosmid
genomic library
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Corresponding Author(s):
GUO Wei,Email:guowei@hebau.edu.cn; LIU Daqun,Email:ldq@hebau.edu.cn
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Issue Date: 05 December 2011
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