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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2011, Vol. 5 Issue (4) : 666-670    https://doi.org/10.1007/s11703-011-1130-2
RESEARCH ARTICLE
Potential of Beauveria bassiana for biologic control of Apriona germari
Huiping LI(), Dazhuang HUANG, Zhigang WANG
College of Forestry, Agricultural University of Hebei, Baoding 071001, China; The Trees Germplasm Resources and Forest Protect Key Laboratory of Hebei Province, Baoding 071000, China
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Abstract

Apriona germari is a destructive stem-boring pest. To date, the control of the pest is still based largely on the chemical insecticides. To meet the needs of people’s high pesticide efficacy and environment safety, the alternative management strategy must be proposed. Beauveria bassiana is one of the most widely studied and used entomopathogenic fungi. The potential of Beauveria bassiana used to control Apriona germari was here evaluated. The result showed that the infective rate of B. bassiana to adults was 20%, with no pathogenicity to eggs. But it showed a high pathogenicity to larvae. The laboratory bioassays showed that the mortality, when concentration was 1 × 108 conidia/mL, was 96.47% ten days after inoculation. LT50 of B. beauveria to Apriona germari larvae was 4.53 d at the concentration of 1 × 108 conidia/mL; LC50 was 6.76 × 105 conidia/mL. The infecting experiments in field showed that, 20 days after control, the mortality was 68.4%. The present results suggested that B. bassiana has an excellent potential for biological control of A. germari larvae.

Keywords Apriona germari      Beauveria bassiana      biological control      potential     
Corresponding Author(s): LI Huiping,Email:huipingli@sohu.com   
Issue Date: 05 December 2011
 Cite this article:   
Dazhuang HUANG,Huiping LI,Zhigang WANG. Potential of Beauveria bassiana for biologic control of Apriona germari[J]. Front Agric Chin, 2011, 5(4): 666-670.
 URL:  
https://academic.hep.com.cn/fag/EN/10.1007/s11703-011-1130-2
https://academic.hep.com.cn/fag/EN/Y2011/V5/I4/666
TreatmentInoculated numberHatched numberEgg hatchability(%)Dead larvae number 3 days after hatchingInfectivity
Inoculated2521843uninfected
Control181688.891uninfected
Tab.1  Infectivity of to eggs of
TreatmentInoculated numberInfected numberInfection rates(%)
Inoculated25520
Control1800
Tab.2  Infectivity of to adults of
IsolatesCumulate mortality(%)Correct mortality (%)
Bi0596.7±0.0196.47
Tab.3  Mortality of Bi05 to the larvae (10 d after inoculation)
IsolatesLT50±SE(d)95% fiducial limitsSlope±SE
LowerUpper
Bi054.53±0.6313.875.212.13±0.210
Tab.4  LT (in days) of Bi05 to larvae at concentration of 1 × 10 conidia/mL
Concentration of Bi05(conidia/mL)
1 × 1041 × 1051 × 1061 × 1071 × 108
Mortality of larvae (%)0.190.330.520.860.95
Corrected mortality (%)0.110.260.470.840.95
Dose regression equationY = 0.514+ 0.777X(R2 = 0.977), F = 176.82>F0.05(1 3) = 10.13
LC50 (conidia/mL)6.76 × 105
Tab.5  LC value of isolate Bi05 against larvae 10 d after treatment
Fig.1  The control effect of on larvae in the field.
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