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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2011, Vol. 5 Issue (4) : 662-665    https://doi.org/10.1007/s11703-011-1134-y
RESEARCH ARTICLE
Observation of the infection process of Metarhizium anisopliae on the cuticle of Anoplophora glabripennis larvae with scanning electron microscopy
Haixia YAN1,2, Da WANG3, Xusheng ZHAO4, Dazhuang HUANG1,2(), Xiangchao MA1
1. Forestry College, Agricultural University of Hebei, Baoding 071000, China; 2. Key Laboratory of Forest Germplasm and Protection of Hebei Province, Baoding 071000, China; 3. Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 4. International Cooperation Office, Agriculture University of Hebei, Baoding 071000, China
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Abstract

The invasion behavior and infection process of Metarhizium anisopliae on different cuticle areas of Anoplophora glabripennis larvae were observed by scanning electron microscopy. The results indicated that M. anisopliae infected A. glabripennis larvae mainly through the intersegmental membrane where more attaching conidia, faster germination, and higher germination and penetration rates were observed. The secondary invasion area was around the valve. The two areas were vulnerable to infection from M. anisopliae. Twelve hours after vaccination, conidia germinated and bud-shaped protrusions formed on the cuticle of A. glabripennis larvae, then germ tubes and various attaching structures were produced. Conidia penetrated the integument into the hemocoele of A. glabripennis larvae 36–48 h later.

Keywords Metarhizium anisopliae      Anoplophora glabripennis      different areas of cuticle      invasion behavior      infection process      scanning electron microscopy     
Corresponding Author(s): HUANG Dazhuang,Email:huangdazhuang@126.com   
Issue Date: 05 December 2011
 Cite this article:   
Da WANG,Xusheng ZHAO,Dazhuang HUANG, et al. Observation of the infection process of Metarhizium anisopliae on the cuticle of Anoplophora glabripennis larvae with scanning electron microscopy[J]. Front Agric Chin, 2011, 5(4): 662-665.
 URL:  
https://academic.hep.com.cn/fag/EN/10.1007/s11703-011-1134-y
https://academic.hep.com.cn/fag/EN/Y2011/V5/I4/662
Fig.1  Infection progress of on larvae. A shows gemmiform protuberance (arrow) appearing on the top of the conidia upon integument 12 h post-inoculation; B–C indicate aspersoria penetrating the integument, resulting from conidia germination at the edge of the valve and intersegmental membrane 12 h post-inoculation; D indicates aspersoria resulting from conidia penetrating the integument,16 h post-inoculation; E–F indicate a large amount of germ tubes and various attaching structures covered with mucilage secretion penetrating the integument, 20 h post-inoculation. E shows the area around the valve; F is the body wall; G–H indicate conidia penetrating the integument, the depression (arrow) at the point of penetration, 24 h post-inoculation; I–J indicate oriented growth of some germ tubes toward the cuticle and penetration into the cuticle. I is 36 h post-inoculation; J is 48 h post-inoculation; K is 48 h post-inoculation, shows long hyphae from conidia germination in the valve and penetration; L shows conidia and hyphae had disappeared and the attachment exfoliated at the intersegmental membrane of the just dead larvae; M–N show a few hyphae grew out of the surface and the valve fringe of the dead larvae, 72 h post-inoculation; O–P show extensive hyphae growth out of the integument; Q shows intensive hyphae growth out of intersegmental membrane; and R means intensive hyphae growth out of the open valve of the just dead larvae.
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