Study on separation and purification of oligomeric proanthocyanidin from <italic>Rhodiola rosea

doi:10.1007/s11703-011-1137-8

Front Agric Chin

2011, Vol. 5

Issue (4) : 637-642 https://doi.org/10.1007/s11703-011-1137-8

RESEARCH ARTICLE

Study on separation and purification of oligomeric proanthocyanidin from Rhodiola rosea

Zhiping YIN¹, Boyang ZHANG², Hongyu CHEN¹, Sisi WANG¹, Wen ZHAO¹(

)

1. College of Food Science and Technology, Hebei Agriculture University, Engineering Technology Research Center for Agricultural Product Processing of Hebei, Baoding 071001, China; 2. College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China

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Abstract

The objective was to study the technology for the separation and purification of oligomeric proanthocyanidins from Rhodiola rosea and establish the best operating conditions. First, the oligomeric proanthocyanidins was extracted by ethyl acetate from rough-extracted R. rosea liquid using an optimized technique. Its purification was achieved by macroporous resins. Five kinds of macroporous adsorbent resins were compared for the adsorption and desorption performance of procyanidins, the concentration and pH value of both the extracted sample and the eluant were investigated. According to the results, the optimized conditions were as follows: four times of extraction at 25 min each time was effective, the best volume ratio was 1.5:1 (ethyl acetate: extracted solution); AB-8 resin was the best choice; the concentration of the extracted sample was 4.0 g/L, and the pH was 4.5; the ratio of the diameter to height of the chromatography column was 1:40 (cm); 50% ethanol was used as the eluant at pH 5; and finally, the purity of procyanidins reached 88. 3%.

Keywords Rhodiola rosea oligomeric proanthocyanidins extraction macroporous resins separation and purification

Corresponding Author(s): ZHAO Wen,Email:zwgyf@yahoo.com.cn

Issue Date: 05 December 2011

Cite this article:

Boyang ZHANG,Hongyu CHEN,Sisi WANG, et al. Study on separation and purification of oligomeric proanthocyanidin from Rhodiola rosea[J]. Front Agric Chin, 2011, 5(4): 637-642.

URL:

https://academic.hep.com.cn/fag/EN/10.1007/s11703-011-1137-8
https://academic.hep.com.cn/fag/EN/Y2011/V5/I4/637

Tab.1 Factors and levels of orthogonal test

Tab.2 Factors and levels of orthogonal test

Fig.1 Effect of extraction time on the extraction rate.

Fig.2 Effect of volume of ethyl acetate on the extraction rate.

Fig.3 Effect of extraction times on the extraction rate.

Tab.3 Results of orthogonal test on extraction

Tab.4 The variance analysis of orthogonal experiment

Tab.5 The structure characters of resins and their proanthocyanidins adsorption properties

Fig.4 Effect of the concentration of the stock solution on adsorption.

Fig.5 Effect of pH of the stock solution on adsorption.

Fig.6 Effect of eluant concentrations on the desorption rate.

Fig.7 Effect of pH of eluant on desorption rate.

Tab.6 Result of orthogonal test on macroreticular resins

Tab.7 The variance analysis of the orthogonal experiment

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