Effect of a Chinese herbal formula <italic>Astragalus</italic> immunomodulator on immune function of chickens

doi:10.1007/s11703-009-0020-3

Front Agric Chin

2009, Vol. 3

Issue (2) : 209-215 https://doi.org/10.1007/s11703-009-0020-3

RESEARCH ARTICLE

Effect of a Chinese herbal formula Astragalus immunomodulator on immune function of chickens

Qingru ZHANG, Guojun JIANG, Shupeng LI, Xiuhui ZHONG(

)

College of Traditional Chinese Veterinary Medicine,Agricultural University of Hebei,Dingzhou 073000, China

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Abstract

In order to investigate the immunomodulatory effects and the mechanism of a Chinese herbal medicine, Zengmiansan (Astragalus immunomodulator), on immune function of chickens, three hundred 1-day-old chickens were assigned randomly into 5 groups, i.e., the blank control group, the Chinese herbal medicine Zengmiansan (ZMS)-treated group, the cyclophosphamide group, the cyclophosphamide plus levamisole group, the cyclophosphamide plus ZMS group and the control group. All chickens were immunized with Lasota vaccine by nose-drip or eye-drop at the age of 6 days. Newcastle Disease (ND) antibody titers, growth indexes of the spleen, thymus and bursa of Fabricius, the concentrations of CD₄⁺ lymphocytes and CD₈⁺ lymphocytes in spleen, thymus and peripheral blood, and the apoptosis of splenocytes, thymocytes and bursa of Fabricius cells were observed at the ages of 14, 21, 28 and 35 days, respectively. Our results indicated that the NDV antibody titers of chickens in the Chinese herbal medicine ZMS-treated group at the ages of 14 and 21 days were significantly higher than that of the other groups (P<0.01). The growth indexes of immune organs, the concentrations of CD₄⁺ lymphocytes and the ratio of CD₄⁺/CD₈⁺ T lymphocytes of chickens in the Chinese herbal medicine ZMS- treated group at the ages of 14, 21 and 28 d were significantly higher than those of the other groups (P<0.01). The apoptotic splenocytes, thymocytes and bursa of Fabricius cells of chickens in the ZMS-treated group were significantly lower than the other groups (P<0.01) at the ages of 14, 21 and 28 days.

Keywords chicken Chinese herbal medicine apoptosis immune

Corresponding Author(s): ZHONG Xiuhui,Email:zhongxiuhui@hotmail.com

Issue Date: 05 June 2009

Cite this article:

Qingru ZHANG,Guojun JIANG,Shupeng LI, et al. Effect of a Chinese herbal formula Astragalus immunomodulator on immune function of chickens[J]. Front Agric Chin, 2009, 3(2): 209-215.

URL:

https://academic.hep.com.cn/fag/EN/10.1007/s11703-009-0020-3
https://academic.hep.com.cn/fag/EN/Y2009/V3/I2/209

Tab.1 Effects of Chinese herbal medicine on growth indexes of spleen, thymus and bursa of Fabricius

Tab.2 Effects of Chinese herbal medicine on NDV antibody titers

Tab.3 Effects of Chinese herbal medicine on the concentrations of CD lymphocytes

Tab.4 Effects of Chinese herbal medicine on the concentrations of CD lymphocytes

Tab.5 Effects of Chinese herbal medicine on the ratios of CD/CD lymphocytes

Tab.6 Effects of Chinese herbal medicine on the apoptosis of splenocytes , thymocytes and bursa of Fabricius cells

1	Abbas A K, Murphy K M, Sher A (1996). Functional diversity of helper T lymphocytes. Nature , 383(6603): 787-793 doi: 10.1038/383787a0
2	An S L, Zhang S Y, Piao H S (2007). Effects of polysaccharides from the annual Astragaluson immuno-organs coefficients in mice. Journal of Medical Science Yanbian University , 30(1): 20-22 (in Chinese)
3	Cai X Y, Xu Y L, Lin X J (2006). Effects of Radix astragaliinjection on apoptosis of lymphocytes and immune function in patients with systemic lupus erythematosus. Journal of Integrated Traditional and Western Medicine , 26(5): 443-445 (in Chinese)
4	Chen G H, Pan G H, Shen S S, Meng X F, Zhou R H (2006). Effect of heshouwu and roucongrong compound on immunological function of elderly patients with kidney deficiency and blood stasis. Chinese Journal of Clinical Rehabilitation , 10(27): 13-15 (in Chinese)
5	Kamalavenkatesh P, Vairamuthu S, Balachandran C, Manohar B M, Raj G D (2005). Immunopathological effect of the mycotoxins cyclopiazonic acid and T-2 toxin on broiler chicken. Mycopathologia , 159(2): 273-279 doi: 10.1007/s11046-004-7321-0
6	LiN, She R P, Han L J, Wang K Z (2004). Effect of Astragalus root extractions on chicken growth and immunological function. Chinese Journal of Veterinary Science and Technology , 34(5): 61-64 (in Chinese) doi: 10.1360/03ye0135
7	Shu X, McCulloch M, Xiao H, Broffman M, Gao J (2005). Chinese herbal medicine and chemotherapy in the treatment of hepatocellular carcinoma: A meta-analysis of randomized controlled trials. Integr Cancer Ther , 4(3): 219-229 doi: 10.1177/1534735405279927
8	Strong J, Wang Q, Killeen N (2001). Impaired survival of T helper cells in the absence of CD₄. Proc Natl Acad Sci USA , 98(5): 2566-2571 doi: 10.1073/pnas.051329698
9	Wei Y M, Chen Z H; Xing G H (2000). Protective Effect of Jade screen Decoction on Immune Organs in immunosuppressive Mice. Chinese Journal of Veterinary Science , 20(2): 181-182 (in Chinese)
10	Wei X Y, Zhang J K, Luo Y H, Chen S B, Sun J L (2000). Antagonistic Effect of Astragalus mongolicus and Polygonum multiflorum on Thymocytes Apoptosis Induced by Cyclophosphamide. Journal of China Pharmaceutical University , 31(1): 35-38 (in Chinese)
11	Wu G Q, Wang X N, Zhang H M (2004). Studies on effect of traditional Chinese medicine on apoptosis. Hebei Journal of Traditional Chinese Medicine , 26(11): 879-880 (in Chinese)
12	Xia H S, Li K S, Zhang Y F, Jia X Q, Li Y H (2001). Effect of Astragalus polysaccharide on DXM-induced spleen lymphocyte apoptosis. Shenzhen Journal of Integrated Traditional and Western Medicine , 11(4): 193-199 (in Chinese)
13	Xie Y X, Song Z Y (2005). Causes and control measures of ND immunization failure. Livestock and Poultry Industry , 178(2): 21 (in Chinese)
14	Xu D J, Chen M Z (2006). The experimental study of TAE on the immune-regulatory effects in mice. Pharmacology and Clinics of Chinese Materia Medica , 22(6): 40-41 (in Chinese)
15	Xu A X, Wang C Q, Yang S H, Ge B, Zhang Z M (2005). The synergistic effect on antisensitivity and its mechanism of PFR and PB WF. Journal of Lanzhou Medical College (Medical science) , 31(2): 13-16 (in Chinese)
16	Yang Y P, Hu D G, Chen X (2001). The comparison of inhibition by Astragalus Polysaccharide and modified Jade screen decoction on murine thymocyte apoptosis. Journal of Hubei College of TCM , 3(4): 34-35 (in Chinese)
17	Yuan M, Chen X H, Ji Y B (2005). Effects of point injection of astragalus and moxibustion with herbal cake on immunological function in tumor mice. Shanghai Journal of Acupuncture and Moxibustion , 24(3): 40-42 (in Chinese)
18	Zhang J D, Li S F, Yang R D, Yang S Y, Xue Z L, Li K W (2003). Effects of the Chinese herbal medicine immunopotentiator on the immunity with ILT vaccine and virulent strains in chickens. Acta Veterinaria et Zootechnica Sinica , 34(2): 168-171 (in Chinese)
19	Zhang K R, Li X Y, Yu M (2003). Effects of astragalus root on immune organ apoptosis under gut ischemia/reperfusion injury. Journal of China Medical University , 32(6): 584-586 (in Chinese)
20	Zhang Y, Cheng J F, He J R, QinH (2003). Protection of Astragalus polysaccharide on toxicity and bone marrow inhibition caused by cyclophosphamide. Journal of the Fourth Military Medical University , 24(5): 447-448 (in Chinese)
21	Zhu X Y, Chen Y X, Zhong X Y, Zhang X Z, Liu J H, He M (2006). The protective effect of Shenqi Fuzheng Injection on immune function of BALB/c mice after chemotherapy. Chinese Journal of Immunology , 22(10): 925-928 (in Chinese)

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