|
|
|
Cloning and expression analysis of a ribosomal
protein S7 in wheat under the stress of Puccinia triticina |
| Aihua YAN,Yunwei ZHANG,Lifeng ZHANG,Dongmei WANG, |
| College of Life Science,
Agricultural University of Hebei, Baoding 071001, China; |
|
|
|
|
Abstract A gene was isolated from the suppression subtractive hybridization (SSH) cDNA library of wheat by reverse northern blotting. Full length cDNA was obtained by RACE technique, and the result showed that all sequences consisted of 471 bp and encoded 157 amino acid. Subsequently, the gene expression was enhanced according to quantity real-time PCR (qRT-PCR) in response to leaf rust stress as the time over. All the results above suggested that the gene might play important role in wheat hypersensitive response (HR) to leaf rust.
|
| Keywords
wheat
ribosomal protein
cloning
expression
|
|
Issue Date: 05 December 2009
|
|
|
Cashmore A R(1976). Protein synthesis in plant leaf tissue.The sites of synthesis of the major proteins. The Journal of Biological Chemistry, 251(9): 2848―2853
|
|
Chiaruttini C, Milet M, Hayes D H, Expert-Bezancon A(1989). Crosslinking of ribosomal proteins S4, S5, S7, S8, S11,S12 and S18 to domains 1 and 2 of 16S rRNA in the Escherichia coli 30S particle. Biochimie, 71: 839―852
doi: 10.1016/0300-9084(89)90048-5
|
|
Clemens M J(2004). Targets and mechanisms for the regulationof translation in malignant transformation. Oncogene, 23: 3180―3188
doi: 10.1038/sj.onc.1207544
|
|
Du Z R, Xi J, Wan J, Jiang B, Xu Y J, Zhang Q, Xu Z J(2008). Cloning and expression analysis of a ribosomal protein from rice(OsRPL14). Chinese Agricultural Science Bulletin, 24(4): 130―134 (in Chinese)
|
|
Eneas-Filho J, Hartley M R, Mache R(1981). Pea chloroplastribosomal proteins: characterization and site of synthesis. Mol Gen Genet, 184: 484―488
doi: 10.1007/BF00352527
|
|
Espen E, Jan L, Andrew L(2003). Silencing the Drosophilaribosomal protein L14 gene usingtargeted RNA interference causes distinct somatic anomalies. Gene, 320(27): 41―48
|
|
Joseph S, David W R(2001). Molecular Cloning, A Laboratory Manual. 3rd ed. New York: Cold Spring Harbor LaboratoryPress
|
|
Kawasaki S, Borchert C, Deyholos M, Wang H, Brazille S, Kawai K, Galbraith D, Bohnert H J(2001). Gene expression profiles during the initial phase ofsalt stress in rice. The Plant Cell, 13; 889―905
|
|
Kim K Y, Park S W, Chung Y S(2004). Molecular cloningof low-temperature-inducible ribosomal proteins from soybean. Journal of Experimental Botany, 55: 1153―1155
doi: 10.1093/jxb/erh125
|
|
Kongsuwan K, Yu Q, Vincent A, Frisardi M C, Rosbash M, Lengyel J A, Merriam J(1985). A Drosophila Minute gene encodes a ribosomal protein. Nature, 317(6037): 555―558
doi: 10.1038/317555a0
|
|
Li M Y, Hu Q R, Xuan J S, Deng D Y, Wang M L(2002). PRL24mutant terminates expression of λN gene by translation. Science in China (series C), 32(5): 405―411 (in Chinese)
|
|
Paula C, Virginia W(2003). Gene expression profiling in response to ultravioletradiation in maize genotypes with varying flavonoid content. Plant Physiology, 132(4): 1739―1754
doi: 10.1104/pp.103.022871
|
|
Qi Y, Liu G, Hou C Y, Wang D M(2008). The generation of H2O2 in wheat hypersensitive reaction induced by leaf rustfungus. Journal of Molecular Cell Biology, 41(4): 245―254 (in Chinese)
|
|
Sambrook J, Russell D W(2002). Molecular Cloning, A Laboratory Manual. 3rded. Beijing: Science Press(in Chinese)
|
|
Thomas B, Michael U, Jurgen F(2000). cDNAcloning of cytoplasmic ribosomal protein S7 of winter rye (Secale cereale) and its expression in low-temperature-treatedleaves. Biochim Biophys Acta, 1492(1): 276―279
|
|
Wool I G(1996). Extraribosomal functions ribosomalproteins. Trends Biochem Sci, 21(5): 164―165
|
|
Xu C S, Yang Z L, Dong H M(2008). The similaritiesand differences in expression of ribosome-related genes between ratregeneration liver and liver tumor. ActaAnatomica Sinica, 39(3): 281―286 (in Chinese)
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
